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Citations for Agilent :
301 - 350 of 418 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The supernatant and extract fractions were dissolved in 0.1% TFA solution in deionized water and applied to Agilent Prep 5 C18 column (10 x 250 mm, particle size 5 μm, Agilent Technologies). The processed McCYps and McCEco were first purified in 0.1% TFA/acetonitrile system in a linear 1-13% gradient of acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μL mL−1 consisting of a 2 min hold at 10% B ...
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bioRxiv - Neuroscience 2022Quote: ... samples were injected into a C18 reverse phase column (Atlantis T3, 2.1 × 150 mm, 3 μm; Waters) using HPLC (Agilent 1290 LC) at a flow rate of 0.15□ml/min with 5 mM ammonium formate for mobile phase A and 100% methanol for mobile phase B ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were re-dissolved in 100 µL solvent A (0.1% TFA in water/ACN (98:2, v/v)) and injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
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bioRxiv - Biochemistry 2022Quote: ... with additional semi-preparative reverse phase high-performance liquid chromatography purification for TAZ1 carried out on a C8 column using a water: acetonitrile gradient (Zorbax, Agilent Technolgies). All protein purification fractions were monitored by SDS-PAGE.
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bioRxiv - Bioengineering 2023Quote: ... the slides were washed under cold running water for 10 min and then coverslips were mounted with glycergel (Dako, Hamburg, Germany). As an additional lipid visualization ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
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bioRxiv - Synthetic Biology 2024Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity - Waters, USA) on a C-8 column (Zorbax Eclipse XBD - Agilent, USA) at a flow rate of 0.6 mL/min and eluted off the column using a hydrophobicity gradient ...
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bioRxiv - Bioengineering 2019Quote: ... Then endogenous peroxidase block was performed by using 3% H2O2 in water to cancel the interference of peroxidase in the final step.[33] Protein block buffer (Dako Protein Block) was used to treat the tissue slides to mask the non-specific binding sites ...
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bioRxiv - Biophysics 2021Quote: ... (buffer A: 0.1% trifluoro-acetic acid in water, buffer B 100% acetonitrile with 0.1% trifluoro-acetic acid; Agilent Zorbax 300SB-C18 column). Collected protein fractions were lyophilized and stored at −20 °C.
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bioRxiv - Immunology 2021Quote: ... An Agilent 6495 UHPLC chromatograph equipped with a Waters Acquity BEH 1.7 μm, 2.1 x 100 mm column (Waters Corporation, Milford, USA) coupled to a QqQ-MS/MS (Agilent Technologies, Atlanta, GA, USA) was used ...
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bioRxiv - Biochemistry 2021Quote: ... The digestion and desalting (total time 3 min) were driven by 0.4% formic acid (FA) in water pumped by 1260 Infinity II Quaternary pump (Agilent Technologies, Waldbronn, Germany) at a flow rate of 100 μL·min−1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The filtrates were resuspended in 100 μL Milli-Q water and analyzed using an Agilent CE-TOFMS system (Agilent Technologies, CA, USA). The relative peak area (sample quantity × peak area/area of the internal standard ...
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bioRxiv - Immunology 2019Quote: ... An Agilent 6495 UHPLC chromatograph equipped with a Waters Acquity BEH 1.7 µm, 2.1 x 100mm column (Waters Corporation, Milford, USA) coupled to a QqQ-MS/MS (Agilent Technologies, Atlanta, GA, USA) was used ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded on an Acquity Ultra Performance system (Waters Corporation, Milford, MA, USA; or Varian 920 LC machine, Agilent, Stockport, UK) equipped with a ZORBAX 300SB-C18 column (Agilent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... to remove insoluble components and diluted 50X times (v/v) in ultrapure water and injected into an Agilent 1100 Series LC (Agilent Technologies, Germany) hyphenated to a Bruker HCT-Ultra ion trap mass spectrometer (Bruker Daltonics ...
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bioRxiv - Genomics 2022Quote: ... Amplified libraries were eluted in 30μL molecular grade water and quantified using a Bioanalyzer DNA 1000 chip (Agilent Technologies, Cat#: 5067-1504).
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bioRxiv - Immunology 2023Quote: ... the sections were submerged in a distilled water bath for 10 min and antigenic recovery was performed with citrate buffer (DAKO, pH 6.0) for 25 min in a water bath at a temperature of 95°C ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The resultant solutions were diluted eleven-fold in deionized water and the absorbance at 760 nm was monitored with UV-vis spectroscopy (Agilent, model 8453). Similar to our previous study,14 the absorbance at 760 nm was background corrected by subtracting the absorbance at 500 nm ...
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bioRxiv - Plant Biology 2023Quote: Lyophilised tissue (10 mg) was extracted with chloroform/methanol/water and analysed with 7890A GC system coupled to a 5795C MS detector (Agilent Technologies, Inc.) in splitless mode with a gradient of 8°C/min ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were analysed with acetonitrile/water/isopropanol-based solvent system (22) by employing Agilent 1290 Infinity HPLC (Agilent Technologies, Santa Clara, CA) equipped with a Luna Omega C18 100 Å (50 x 2.1 mm ...
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bioRxiv - Genomics 2021Quote: ... using a mixture of equal volume of ready-to-use protein block serum-free solution (Agilent Dako, cat.no ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were rinsed in PBS three times and incubated in serum free DAKO Protein Block (X0909, DAKO) for 1h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were processed as free-floating and stained with rabbit polyclonal anti-GFAP (1:2000 dilution, DAKO Z0334 ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained using the CSA II kit (Biotin-free catalysed signal amplification system; DAKO, Glostrup, Denmark) and 3,3’-diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2020Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Free AA profile was analysed using an HPLC equipped with a UV-VIS detector (Agilent 1100, USA) at 338 nm ...
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bioRxiv - Cell Biology 2022Quote: ... cells or tissue sections were incubated with Protein Block Serum-Free Ready-To-Use (Dako, #X 0909) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and slides were incubated with Dako Serum Free Protein Block (#X090930-2, Agilent, Santa Clara, CA, USA) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... All lines were tested monthly and verified as being mycoplasma-free (MycoSensor PCR Assay Kit, Agilent Technologies).
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bioRxiv - Biochemistry 2022Quote: ... The assay medium consisted of bicarbonate free DMEM medium pH 7.4 (Agilent Technologies, Santa Clara, CA, USA) supplemented with 2% of FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were mounted on SuperFrost+ slides then coverslipped with fluorescent mounting medium (Agilent, S302380-2) and #1.5 coverslips (dx.doi.org/10.17504/protocols.io.b5s5q6g6).
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were mounted on SuperFrost+ slides then coverslipped with fluorescent mounting medium (Agilent, S302380-2) and #1.5 coverslips (dx.doi.org/10.17504/protocols.io.rm7vzxbz4gx1/v1).
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bioRxiv - Immunology 2022Quote: ... The samples were then analyzed with GC-TOF/MS (Waters, Milford, MA) with an electron impact mode and a DB-5MS column (Agilent, Santa Clara, CA) following our published protocol (39) ...
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bioRxiv - Plant Biology 2022Quote: ... Metabolites were then re-suspended in 100 μL distilled water and soluble sugars and sugar alcohols (fructose, glucose, sucrose, mannitol, sorbitol) were quantified by HPLC (Agilent 1260 Infinity II) using an 8 μm Hi-Plex Ca column (7.7 mm x 300 mm ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Cancer Biology 2020Quote: ... pre-coated XF96 plates at 105 cells/well in 175 μL of sodium bicarbonate-free XF Base (Agilent) media freshly supplemented with 10 mM glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X 5 mins with 1X PBS and blocked in DAKO protein-free serum block (DAKO) overnight in a humidified chamber at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The processing of NMR free induction decay data was conducted using VNMRJ3.2a (Agilent Technologies Inc., Palo Alto, CA). Spectral analysis was then conducted using graphical assignment utilities CCPNMR and Sparky-NMRFAM121-122.
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bioRxiv - Neuroscience 2023Quote: Free-floating brain tissue was stained for polyclonal rabbit anti-glial fibrillary acidic protein primary antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Microbiology 2020Quote: ... Samples were analyzed by high-performance liquid chromatograph (HPLC; Waters 2795, Mississauga, ON, Canada) equipped with a C8 reverse-phase column (Eclipse XDB-C8, Agilent Technologies, Mississauga, ON, Canada), and the detector was a tandem quadrupole mass spectrometer (Quattro Premier XE ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Molecular Biology 2023Quote: ... (https://www.shimadzu.com.cn/) system composed of Waters Acquity I-Class instrument equipped with UPLC HSS T3 column (Agilent SB-C18, 1.8μm, 2.1 mm × 100 mm). For positive and negative ion mode (ESI-MS) ...