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Citations for Agilent :
51 - 100 of 2476 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... C4b deposition was measured using a biotin-tagged anti-C4c polyclonal Ab (Agilent, Q0369) followed by HRP-streptavidin and TMB One ...
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bioRxiv - Cancer Biology 2021Quote: ... and a commercially available tyramide-based avidin-biotin amplification system (Catalyzed Signal Amplification System, DakoCytomation). IRDye 680RD streptavidin (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by labeling with streptavidin biotin/horseradish peroxidase (LSAB2 System HRP, Dako, Santa Clara, USA) and Diamino-benzidine (DAB ...
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bioRxiv - Neuroscience 2021Quote: ... Next day membranes were washed for 3 x 5 min and incubated with HRP-coupled secondary antibodies (DAKO) diluted 1:3000 in washing buffer for 1.5 hour at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-μm sections were stained with antibodies or control IgG using the EnVision+System-HRP (DAB) kit (Dako North America Inc ...
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bioRxiv - Bioengineering 2022Quote: ... Samples of labeled and unlabeled antibody (5 – 10 μg) were run on a HPLC (Agilent 1260 Infinity II) using an Agilent AdvanceBIO SEC 300Å 2.7 mm column (PL1580-3301 ...
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bioRxiv - Bioengineering 2019Quote: ... or manually using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, DakoCytomation). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Immunology 2023Quote: ... Tetramers were assembled by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Agilent, #PJRS27-1) according to NIH Tetramer Facility protocols ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained using the CSA II kit (Biotin-free catalysed signal amplification system; DAKO, Glostrup, Denmark) and 3,3’-diaminobenzidine (DAB) ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were made by conjugating the biotin-labeled monomers with PE-labeled or APC-labeled streptavidin (Agilent) according to protocols from the NIH tetramer facility ...
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bioRxiv - Immunology 2020Quote: ... The tetramers were made by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Prozyme, Hayward, CA) according to protocols from the NIH tetramer facility ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 min or with 0.4 µg/ml anti-human cath-D mouse monoclonal antibody (clone C-5) for 20 min after heat-induced antigen retrieval with the PTLink pre-treatment (Dako) and the High pH Buffer (Dako ...
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 2 hours at room temperature with polyclonal guinea pig anti-insulin antibody (1:5, Agilent Technologies). Slides were then washed 3 x 5 minutes with PBS and incubated with the Anti-guinea pig IgG Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... three 5 μm-thick tissue sections separated by 300 μm were stained with guinea pig anti-insulin antibody (undiluted, Dako IR002 ...
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p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional responsebioRxiv - Cancer Biology 2021Quote: ... The cells were washed 3 times with PBS for 5 min and then incubated with a secondary antibody: Flex-HRP (EnVision System, Dako) for 30 min and washed 3 times 5 min with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times (5 min) in TBST prior to addition of secondary antibody (goat anti-rabbit HRP 1:10,000; Dako - P00448). Blots were washed 3 times in TBST prior to development using SuperSignal West Pico Plus substrate (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Signal amplification was achieved using a commercially available tyramide- based avidin/biotin amplification system (Dako Cytomation, Carpinteria, CA) coupled with fluorescent detection using the streptavidin-conjugated IRDye680 dye (LI-COR Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µm (Agilent). Next ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was performed using the streptavidin-biotin-peroxydase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed thrice for 5 min in TBS-T and incubated with Horseradish peroxidase secondary antibodies (DAKO, Santa Clara, USA) for 1 hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... 5[μm column (Agilent).The mobile phases were MS solvent A (H2O ...
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bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
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bioRxiv - Cancer Biology 2021Quote: Biopsy sections were deparaffinized and antigens were retrieved by boiling in 10mM sodium citrate buffer pH6 for 40 minutes followed by endogenous biotin blocking (Agilent) and normal goat serum blocking (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with Dako biotin (cat. no. X0590) and peroxidase (cat. no. S200389) blocking reagents (both Agilent, Santa Clara, CA, USA), and incubated with primary antibodies (diluted in TBS-T-BSA ...
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bioRxiv - Cancer Biology 2020Quote: EGFR-KD from Expi293 cell lysates was bound to streptavidin resin and eluted in biotin buffer according to manufacturer’s recommendations (InterPlay Mammalian TAP System; Agilent Technologies). Typical protein yield was between 100-400 ng/µl ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Microbiology 2020Quote: ... the amount of dT10-PEG2-dT10-10% Biotin strands per NP was estimated using a Varian Cary 100 Bio UV-visible spectrophotometer (Agilent Technologies) and a quartz cuvette of 1 cm path length.
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl C18 Cartridges (Agilent) were used as solid phase ...
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bioRxiv - Immunology 2020Quote: ... 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h.
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Pathology 2020Quote: ... Antibodies were diluted in antibody diluent (Dako). Immunostained samples were analyzed with a fluorescence microscope (Olympus DP72) ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies prepared in antibody diluent (Agilent) were added for 8 hours at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were first loaded onto a C18 trap column (5 µm, 5 × 0.3 mm, Agilent Technologies) and then eluted into a C18 analytical column (75 μm × 150 mm ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... 5 μm particle size (Agilent Technologies) was employed for metabolite separation with a linear gradient of 95:5 A/B to 30:70 A/B over 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5% mouse serum (Agilent, Germany). Positive cells were isolated with the micromanipulator and subjected to whole genome amplification.
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bioRxiv - Cell Biology 2020Quote: ... blocked in 5% donkey serum (Dako) in PBS and incubated with primary antibody (α-TFAM (Mouse ...