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Citations for Agilent :
451 - 500 of 566 citations for TREML2 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed using the Exome Cancer Test v2.0 (EXaCT-2) assay that was developed with Agilent based on SureSelect Human All Exon V6 (Agilent Technologies) (manuscript in preparation) ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Patient M03’s DNA was captured with the SureSelect Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced on a NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA genomic libraries were prepared from fresh tissue (tumor and nontumor) and FFPE tumor tissue DNA prior to exome capture (SureSelect XT Human All Exon v5, Agilent). Libraries were sequenced on a HiSeq2000 instrument (Illumina ...
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bioRxiv - Immunology 2024Quote: To accurately investigate the bioenergetic function of bovine and human MØ with an extracellular flux analyser (Seahorse Bioscience, Inc, USA), both cell types were characterized according to the manufacturer’s recommended basal and test conditions (23).
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed as before and incubated with 50 µl/well polyclonal rabbit anti-human IgG conjugated with horseradish peroxidase (Dako, Denmark) at 0.5 µg/ml for another hour at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 1.65 μg cRNA from each labeling reaction was hybridized to the Agilent Whole Human Genome Oligo Microarray (Agilent Technologies, Tokyo, Japan). The Whole Human Genome Oligo Microarray is in a 4 x 44k slide format and each block represents more than 41,000 unique human genes and transcripts ...
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bioRxiv - Genetics 2021Quote: ... and total RNA (ribosomal RNA depleted, toRNA-seq) obtained from an adult human cortex [66-year-old female (540005, Agilent Technologies)] and an adult mouse cortex [eight-week-old (C57BL/6J ...
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from each clone was analyzed by microarray-based comparative genomic hybridization using catalog whole-genome microarrays (SurePrint G3 Human CGH microarray, 8 × 60 k format, Agilent technologies) and/or by multiplex ligation dependent probe amplification using the SHOX SALSA MLPA Probemix (assay ID ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA from isolated RNA as well as a Universal Human Reference RNA that was used as negative control or for normalization (HREF, Stratagene/Agilent # 740000) was performed using an oligo-dT primed SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Transcriptome analysis of primary human HSC was performed by OakLabs (Hennigsdorf, Germany) using Agilent Microarray XS (Agilent Technologies, Santa Clara, USA). Low Input QuickAmp Labeling Kit (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: Trio WES of 13 patients and their parents was performed using the SureSelect Human All Exon V5+UTR kit (Agilent technologies). In patient 4 ...
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bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for preparing the samples for sequencing and SSELXT Human All exon V6 +UTR probes (Agilent Technologies, California, United State) were used for the exome capture ...
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bioRxiv - Genomics 2019Quote: Paired-end DNA sequencing libraries of 28 individuals were generated using Aglilent SureSelect Human All ExonV6 kit (Agilent Technologies, CA, USA) by Novogene Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The library was then enriched by liquid phase hybridization using Agilent SureSelect XT Human All Exon v6 (Agilent Technologies, CA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...