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Citations for Agilent :
451 - 500 of 601 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Cancer Biology 2021Quote: ... Animals treated with CAR T cells had tumors excised and were also stained with H&E and human CD3 (A0452, Dako), in addition to GPC2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries and hybridization capture were performed following the SureSelectXT HS recommended protocol with SureSelect Human All Exon V6+UTR baits (Agilent). Indexed libraries were sequenced on an Illumina NovaSeq with the S4 flow cell (Illumina ...
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bioRxiv - Genomics 2022Quote: ... the three pools were spiked in a diagnostic WES workflow containing 186 exomes (SureSelectXT Low Input Target Enrichment System, Human All Exon V7 probes, Bravo Automated Liquid Handling Platform, Agilent), and 2 whole genome preparations (NEXTFLEX Rapid XP DNA-seq kit ...
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bioRxiv - Neuroscience 2022Quote: ... Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies, pH 9.0) under heat ...
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bioRxiv - Neuroscience 2021Quote: Exonic sequences were enriched with the SureSelect Human All Exon 50□Mb V5 Kit (Agilent Technologies, Santa Clara, CA, USA). Sequences were generated on a HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Each RNA sample was processed with fluorescence-labelling to prepare complimentary RNA (cRNA) targets that could be detected by an SBC human ceRNA array V1.0 (4□×□180K, Agilent Technologies). The microarray kit contained 88,371 circRNA probes and 18,853 mRNA probes ...
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bioRxiv - Cancer Biology 2019Quote: ... Exome capture and sequencing library preparation were performed using Agilent SureSelect Human All Exon V5 (Agilent Genomics, Santa Clara, CA), following standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A, RIOK2123A,246A) were created using site directed mutagenesis (Lightning Quick Change Kit, Agilent) to make alanine substitutions at catalytic residues Lys-123 and Asp-246 in both human RIOK2 and dRIOK2 as described by other groups (22 ...
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bioRxiv - Immunology 2020Quote: ... Cytospots were stained by May-Gruenwald Giemsa stain and mast cells were detected immunohistochemically (monoclonal mouse anti-human mast cell tryptase, Dako). Staining protocols and further details can be found in the supplementary material ...
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bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
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bioRxiv - Cancer Biology 2022Quote: ... WES libraries were generated by exome capture of approximately 20,000 coding genes using SureSelect human All exon V6 kit (Agilent Technologies) and paired-end sequencing was performed on a HiSeq sequencer (Illumina ...
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bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... For cDNA expression microarray analysis using SurePrint G3 Human GE 8×60K Ver.2.0 (Agilent Technologies, Santa Clara, CA, USA), total RNA was sent to a commercial service (Hokkaido System Science ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-4 μm slides were cut and stained for MLH1 (Monoclonal Mouse Anti-Human; Clone ES05, dilution 1 : 10,Dako), MSH2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Genomics 2019Quote: The FLNC reads from the Isoseq3 refine step were error corrected using Lordec (-k 31 -s 3) with short read RNAseq data from the Universal Human Reference RNA (Agilent) (https://www.ncbi.nlm.nih.gov/sra/SRX1426160 ...
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bioRxiv - Cell Biology 2019Quote: ... total leukocyte DNA was enriched using the Agilent SureSelect Human All Exon 50 Mb capture kit (Agilent Technologies, SantaClara, CA). Bidirectional 100-bp paired-end sequencing (Illumina Hi-Seq 2000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Quantitative determination of diphenhydramine in human plasma was accomplished by the use of high-performance liquid chromatography with tandem mass spectrometry detection (Agilent Series 1100 LC with MS detector API4000 ...
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Genomics 2021Quote: ... the initial concentrations of purified SARS-CoV-2 and the universal human reference RNA (UHRR, Agilent Technologies, product number 740000) were determined by ddPCR as described above ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... exome enrichment was performed using the SureSelect Human All Exon 50 Mb Kit V5 (Agilent Technologies, Santa Clara, CA, USA). Sequencing was done on a HiSeq4000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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bioRxiv - Genomics 2023Quote: ... Hybrid capture of exonic regions was performed using Agilent SureSelect in-solution capture reagents and SureSelect XT Human All Exon V4 probes (Agilent). Captured libraries were sequenced using 100 bp paired-end runs on Illumina HiSeq 2500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Cancer Biology 2023Quote: ... exome capture was performed on 200 ng DNA using a customised version of the Agilent Human All Exome V5 Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cytokeratin 5/6 (mouse monoclonal) at 1:100 to 1:200 dilution for zebrafish tissue and 1:100 dilution for human tissue (Dako), GATA 3 (SAB2100898 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed using the Exome Cancer Test v2.0 (EXaCT-2) assay that was developed with Agilent based on SureSelect Human All Exon V6 (Agilent Technologies) (manuscript in preparation) ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Patient M03’s DNA was captured with the SureSelect Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced on a NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA genomic libraries were prepared from fresh tissue (tumor and nontumor) and FFPE tumor tissue DNA prior to exome capture (SureSelect XT Human All Exon v5, Agilent). Libraries were sequenced on a HiSeq2000 instrument (Illumina ...
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bioRxiv - Immunology 2024Quote: To accurately investigate the bioenergetic function of bovine and human MØ with an extracellular flux analyser (Seahorse Bioscience, Inc, USA), both cell types were characterized according to the manufacturer’s recommended basal and test conditions (23).
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...