Labshake search
Citations for Agilent :
1 - 50 of 1899 citations for TBC1 domain family member 3G TBC1D3G Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... FITC secondary antibody (Dako) was incubated for 1h in obscurity at RT followed by propidium iodide for 5min ...
-
bioRxiv - Molecular Biology 2024Quote: An oligonucleotide library containing 21,210 members was synthesized by Agilent (Supplementary Table 8). Each library member contained a spacer sequence ...
-
bioRxiv - Microbiology 2024Quote: ... FITC label was detected with a peroxidase labelled rabbit anti-FITC antibody (DAKOCytomation, Glostrup, Denmark). The signal was amplified with a tyramide signal amplification system (Perkin Elmer ...
-
bioRxiv - Physiology 2021Quote: ... Anti-mouse and anti-rat IgG-FITC antibodies were from Dako and the GAPDH antibody from Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... The following antibodies were used: Ki-67 FITC (clone mip1; Dako), CXCL13 APC (clone 53610 ...
-
bioRxiv - Pathology 2021Quote: ... anti-albumin antibody FITC-coupled was added (DAKO F0117, 1/500).
-
bioRxiv - Bioengineering 2020Quote: A computationally designed 200,000-member naïve nanobody CDR3 library was synthesized as an oligonucleotide pool of CDR3 sequences by Agilent, as previously reported (29) ...
-
bioRxiv - Microbiology 2023Quote: ... A FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody purchased from DAKO and a nonconjugated normal rabbit IgG control antibody purchased from Santa Cruz Biotechnology (sc-3888 ...
-
bioRxiv - Cell Biology 2021Quote: The CC and ORD domains of ORP5 or the TM domain of ORP8 were deleted using site-directed mutagenesis (Quickchange II-XL, Stratagene) to generate EGFP-ORP5ΔCC and EGFP-ORP5ΔORD or EGFP-ORP8ΔTM.
-
bioRxiv - Cancer Biology 2022Quote: ... USA) and the secondary antibody rabbit anti-mouse-FITC (1:50, #F0232, Dako) as previously described [17] ...
-
bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... III14 and IV9 in this family by using SureSelect Human All Exon Kit (Agilent, Santa Clara, CA) to capture the exome and HiSeq2000 platform (Illumina ...
-
bioRxiv - Cancer Biology 2020Quote: ... The following primary antibodies were used: rabbit FITC-anti-Lyz (1:400, Dako, F037201), rabbit anti-Muc2 (1:50 ...
-
bioRxiv - Plant Biology 2024Quote: ... and ligated into the activation-domain vector pAD-Gal4 (Stratagene) using T4 DNA ligase (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2023Quote: ... rabbit anti-FITC (Dako) and Brightvision goat anti-rabbit HRP (Immunologic ...
-
bioRxiv - Immunology 2021Quote: ... FITC anti-human CD44 (DAKO), Purified NA/LE mouse anti-human CD253 (BD Biosciences) ...
-
bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
-
bioRxiv - Pathology 2020Quote: ... used at a dilution of 1:50 and a FITC-conjugated rabbit polyclonal antibody to IgA (Dako, F0204). The polyclonal sheep anti-nephrin antibody was also directly conjugated to Alexa-488 using NHS chemistry ...
-
bioRxiv - Developmental Biology 2022Quote: ... Repeated washes were performed before incubation with secondary antibody (FITC-labelled swine anti-rabbit F (ab’)2 (Dako) or Alexa Fluor 647-labelled goat anti-rabbit IgG (Molecular Probes ...
-
bioRxiv - Biochemistry 2024Quote: ... K101N mutations in pBAD24 using the GeneMorph II EZClone Domain Mutagenesis Kit (Stratagene) with the manufacturer’s protocol and the following primers ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Random mutagenesis was performed using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies and dilutions used were as follows: anti-Mouse Immunoglobulins/FITC (1:400, F0479, Dako, Carpinteria, CA, USA) and anti-Rabbit IgG (H+L ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Error-prone PCR was performed using the GeneMorph II EZClone Domain Mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: The smcR mutant alleles were created using GeneMorph II EZClone Domain Mutagenesis Kit (Stratagene) as per the company protocol targeting the smcR gene encoded on plasmid pJN22 ...
-
bioRxiv - Genomics 2021Quote: ... cytoplasmic MPO-FITC (Dako Cat# F071401-1), and CD33-PE-Cy7 (BD Cat# 333946) ...
-
bioRxiv - Cell Biology 2021Quote: ... The Telomere PNA FISH Kit/FITC (DAKO) was used to probe for telomeres ...
-
bioRxiv - Cancer Biology 2021Quote: ... Polyclonal rabbit anti-mouse immunoglobulins/FITC (DAKO) were used as secondary antibodies for STn detection at a 1:100 dilution in PBS 2% FBS for 15 minutes at room temperature ...
-
bioRxiv - Genetics 2024Quote: ... Whole exome sequencing was performed on genomic DNA extracted from the peripheral lymphocytes of the patients and their families using the SureSelect Human All Exon Kit V6 (Agilent Technologies, Santa Clara, CA, USA) with sequencing on the NovaSeq 6000 platform (Illumina ...
-
Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: Libraries of ProQ mutants were generated by using GeneMorph II EZClone Domain Mutagenesis Kit (Agilent). As template ...
-
bioRxiv - Cancer Biology 2022Quote: ... EZH2’s SET domain deletion was generated by a site-directed mutagenesis kit (Agilent, 200521). All plasmids were verified by Sanger sequencing ...
-
bioRxiv - Plant Biology 2024Quote: ... or bHLH domains of HIVE and BREVE were cloned in pBD-GAL4 Cam (Stratagene, USA). Different combinations of bait and prey plasmids were transformed into yeast strain AH109 using PEG/LiCl method (Clontech ...
-
bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
-
bioRxiv - Immunology 2021Quote: ... CD68-FITC (KP1) (Alligent Dako, Santa Clara, CA) and HLADR-AF594 (L243 ...
-
bioRxiv - Cell Biology 2021Quote: ... the PH domain in HA-ORP5wt was deleted by site-directed mutagenesis (Quickchange II-XL, Stratagene) as in (Galmes et al. ...
-
bioRxiv - Immunology 2020Quote: ... The RBD domain of SARS-CoV-2 S was biotinylated and tetramerized with streptavidin-APC (Agilent). The APC decoy reagent was generated by conjugating SA-APC to Dylight 755 using a DyLight 755 antibody labeling kit (ThermoFisher) ...
-
bioRxiv - Plant Biology 2020Quote: ... Mutations were introduced into the CCT domain by site-directed mutagenesis (Pfu Turbo DNA polymerase, Agilent) and all the constructs were verified by sequencing and subsequently transformed into BL21-CodonPlus (DE3)-RIL Competent Cells for protein production.
-
bioRxiv - Evolutionary Biology 2021Quote: ... Ten μl of FITC PNA telomere probe mix (DAKO) was dropped on the prepared slides and covered with the coverslip ...
-
bioRxiv - Molecular Biology 2024Quote: ... and C3c (1:25, anti-human C3c FITC, Dako F020102) before flow cytometry analysis.
-
bioRxiv - Genetics 2021Quote: Mutagenesis of the sequence encoding Sir3464-728 using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent, 200552-5) was performed by PCR on 9,5 µg of pACT2-SIR3464-728 with 20 cycles of amplification to allow low mutation rate ...
-
bioRxiv - Microbiology 2021Quote: ... Mutageneses of the VqmAPhage and VqmAVc DBDs was accomplished using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The AR DBD domain DNA fragment was amplified using Herculase II Fusion DNA Polymerase (Agilent Technologies; cat. 600677) with 36 cycles and annealing at 63.5°C ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
-
bioRxiv - Biophysics 2023Quote: ... A non-cleavable T855G mutant of this Lphn3 GAIN domain fusion protein was generated using QuikChange II XL (Agilent) site-directed mutagenesis with primers 5’-ATG CAG CTG TAA TCA CCT GGG CAA CTT TGC TGT CCT GAT G -3’ and 5’-CAT CAG GAC AGC AAA GTT GCC CAG GTG ATT ACA GCT GCA T -3’.
-
bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
-
bioRxiv - Cell Biology 2020Quote: ... The cytoplasmic and KASH domains of the mini-nesprin constructs were obtained from a previous publication.22 The mutant domain was made from this construct by site-directed mutagenesis (Quikchange II XL, Agilent). The DN-KASH construct was made by ligation after digestion by ClaI of a PCR product from the mini-nesprin construct ...
-
Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The purified PCR products were subsequently used as megaprimers to generate the library of mutants in the plasmid pZE-ProQ-3×FLAG by following GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) guidelines ...
-
bioRxiv - Microbiology 2021Quote: A library of IpaC mutants with missense mutations in the coiled-coil domain and flanking regions was generated using error-prone PCR with the GeneMorphII (Agilent) domain mutagenesis kit ...
-
bioRxiv - Biophysics 2020Quote: The deletion construct containing the catalytic domain and the interdomain linker (CreCat, residues 127-343) was prepared using QuikChange Site-Directed Mutagenesis Kit (Agilent) from a pET21A vector (Novagen ...
-
bioRxiv - Genetics 2021Quote: ... while the domain deletions were constructs from initial cDNA constructs using the site-directed mutagenesis kit QuikChange™ (Agilent Technologies) and adequate primers (Supplemental Table S4) ...
-
bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...