Labshake search
Citations for Agilent :
101 - 150 of 1164 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Spike-expressing plasmid constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent) on a previously described Wuhan-hu-1 template34 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... then annealing and extension with the conditions as shown in Table S2 for 40 cycles using Mx3000P (Stratagene). Each mRNA level was normalized to the GAPDH mRNA level.
-
bioRxiv - Biophysics 2023Quote: ... We made both handles using primers CD21 and CD22 (Table S2) in a PCR on pBlueScriptSKII SK+ (Stratagene). This was done in the presence of 1/5 biotin-16-dUTP/dTTP or digoxigenin-11-dUTP/dTTP (two separate reactions ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-rabbit and anti-sheep IgG-Peroxidase conjugated (Sigma, cat. A9044 and cat. AP188P and Dako cat. P0163); while the following ones were used for confocal microscopy ...
-
bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
-
bioRxiv - Microbiology 2020Quote: ... The amplicon was ligated in EcoRI/XhoI-digested pCMV-Tag 5A (Stratagene) to create pKozak-PDZD8-myc.
-
bioRxiv - Cancer Biology 2020Quote: ... endogenous biotins and avidins were blocked with corresponding blocking solutions from Dako® ...
-
bioRxiv - Neuroscience 2021Quote: ... Gap43 antibodies were visualized with biotin-conjugated rabbit anti-mouse antibodies (Dako) followed by streptavidin-conjugated Cy3 (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... and immunoreactivities were visualized by the biotin–streptavidin technique (ABC kit; Dako) with 3,3’-diaminobenzidine (DAB ...
-
bioRxiv - Immunology 2024Quote: ... HRP-conjugated or biotin-conjugated secondary antibodies and ABComplex alkaline phosphatase (Dako) were used as secondaries ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotin-containing handles were made using a PCR on pBluescript SK+ (Stratagene) with GoTaq G2 DNA polymerase (Promega ...
-
bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The mutant M1237I spike related constructs were generated using the QuikChange II site-directed mutagenesis kit (Agilent) with the primer set of S-M1237I-F ...
-
bioRxiv - Biochemistry 2021Quote: Mutagenic PCR was used to introduce point mutations in both PgDHAR and HsCLIC1 (Table S2) following manufacturers protocol (Stratagene). 2-5μl of the PCR product was used to transform E ...
-
bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 RBDL Nucleocapsid construct was expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3C library preparation and target enrichment using a custom-designed collection of 6979 biotinylated RNA “baits” targeting single MboI restriction fragments chr15:10283500-13195800 (mm9) (Supplementary Table S2; Agilent Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... a pool of the corresponding oligos (Tables S1 and S2) flanked by adapter sequences (TTCGATATCCGCATGCTAGC-tile-GATCGGAAGAGCACACGTCT) was synthesized (Twist or Agilent). Fragments were PCR-amplified (see Key resources table for primer sequences ...
-
bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... listed in Table S2) and the site directed deletions and amplification were performed using Pfu turbo DNA polymerase (Agilent, #600250) following manufacturers protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... The mutants were prepared by site directed mutagenesis (see Tables S2 and S3 for details) following the QuikChange site-directed protocol (Agilent). For generation of knockin cell lines the pPLOT blast-mNG-blast plasmid was generated with recoded wild type and mutant profilin genes (synthetized by TWIST Bioscience ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gene sequence was PCR-amplified with primers to introduce T7 promoters on both ends (see Table S2, Oligonucleotides tab) and cloned into pSC-A-amp/kan (Stratagene) vector ...
-
bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed with primers (Table S2) and Brilliant Sybr Green QPCR Reagent Kit (Stratagene, Waldbronn, Germany). The ratio of G ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
-
bioRxiv - Cancer Biology 2019Quote: ... the slides were incubated with biotin coupled goat anti-rabbit (1:500, Dako). Subsequently ...
-
bioRxiv - Immunology 2021Quote: ... Biotin-labeled FcRs were then incubated with streptavidin-PE (Prozyme, Hayward, CA, USA) for 10 min and excess streptavidin was quenched with 20 μM biotin (Avidity ...
-
bioRxiv - Plant Biology 2023Quote: ... The slides were then incubated with biotin-conjugated anti-rabbit Ig (Dako E0432) for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... After permeabilization and blocking primary antibody solution using sheep anti Dll1 (Catalog no. AF3970, RnD Systems) and rabbit anti Lysozyme (Catalog no. A0099, Dako) antibodies at a concentration of 1:100 in PBS supplemented with 3 % donkey serum and 0.1 % TritonX was added ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA was sonicated using COVARIS S2 and peak fragment distribution between 100-500bp was verified on an Agilent Bioanalyzer (Agilent Technologies) using the DNA1000 chip ...
-
bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
-
bioRxiv - Immunology 2021Quote: ... Biotinylated tetrameric spike (1ug) and RBD (0.5ug) were fluorochrome linked for flow cytometry by incubating with streptavidin conjugated APC (Prozyme) and PE (Prozyme ...
-
bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
-
bioRxiv - Biophysics 2023Quote: ... we constructed the 5’-biotin handles by a PCR from a pBluescript SK+ (Stratagene) using 5’-biotin primers JT337 and JT338 (Table S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5’-biotin handles were constructed by a PCR reaction from a pBluescript SK+ (StrataGene) using 5’-biotin primers JT337 (Bio-AGAATAGACCGAGATAGGGTTGAGTG ...
-
bioRxiv - Immunology 2024Quote: ... C4b deposition was measured using a biotin-tagged anti-C4c polyclonal Ab (Agilent, Q0369) followed by HRP-streptavidin and TMB One ...
-
bioRxiv - Microbiology 2020Quote: ... The cDNA was sequenced with IonTorrent using a 318 chip (v2) and internal ERCC Spike-In control after ensuring the cDNA quality (Agilent Bioanalyzer 2100 DNA High Sensitivity chip) ...
-
bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
-
bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
-
bioRxiv - Biochemistry 2022Quote: ... All site-directed mutagenesis was performed on this codon-optimized spike clone as the WT form using the QuikChange II Site-directed mutagenesis kit (Agilent), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and a commercially available tyramide-based avidin-biotin amplification system (Catalyzed Signal Amplification System, DakoCytomation). IRDye 680RD streptavidin (LI-COR Biosciences ...
-
bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by labeling with streptavidin biotin/horseradish peroxidase (LSAB2 System HRP, Dako, Santa Clara, USA) and Diamino-benzidine (DAB ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
-
bioRxiv - Microbiology 2020Quote: ... ACE2-Fc variants were generated by the QuikChange II site-directed mutagenesis protocol (Agilent).
-
bioRxiv - Microbiology 2024Quote: The library of the extracted genomic DNA was prepared by Illumina Nextera XT DNA Library Prep Kit protocol (# FC-131-1096) and analyzed by Agilent D1000 ScreenTape ...