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Citations for Agilent :
101 - 150 of 1116 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... After permeabilization and blocking primary antibody solution using sheep anti Dll1 (Catalog no. AF3970, RnD Systems) and rabbit anti Lysozyme (Catalog no. A0099, Dako) antibodies at a concentration of 1:100 in PBS supplemented with 3 % donkey serum and 0.1 % TritonX was added ...
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bioRxiv - Molecular Biology 2021Quote: ... a pool of the corresponding oligos (Tables S1 and S2) flanked by adapter sequences (TTCGATATCCGCATGCTAGC-tile-GATCGGAAGAGCACACGTCT) was synthesized (Twist or Agilent). Fragments were PCR-amplified (see Key resources table for primer sequences ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity was measured using the High Sensitvity RNA ScreenTape Assay in an Agilent TapeStation (Agilent Technologies, Inc.; Fig. S1). The RNA sample (40ng ...
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bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Single variants were introduced by mutagenesis of the Tile 3-KCNH2 plasmid (Table S1) using the Quikchange Lightning Multi kit (Agilent), with 1 primer per variant ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene-specific primers were used (Table S1) together with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies), following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated tetrameric spike (1ug) and RBD (0.5ug) were fluorochrome linked for flow cytometry by incubating with streptavidin conjugated APC (Prozyme) and PE (Prozyme ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... were prepared by double-stranded plasmid mutagenesis using the primer pairs listed in Table S1 and a Quikchange Site-Directed Mutagenesis Kit (Agilent Tech.). After verification by DNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... We performed mutagenesis with the primers (Table S1) designed for the R397W mutation according to the manufacturer’s instruction (QuikChange II Site-Directed Mutagenesis Kit, Agilent Technologies, USA). Upon reaching 50-60% density ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was sequenced with IonTorrent using a 318 chip (v2) and internal ERCC Spike-In control after ensuring the cDNA quality (Agilent Bioanalyzer 2100 DNA High Sensitivity chip) ...
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bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Biochemistry 2022Quote: ... All site-directed mutagenesis was performed on this codon-optimized spike clone as the WT form using the QuikChange II Site-directed mutagenesis kit (Agilent), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification and melting curve analysis (see supplementary Figure S1) and monitoring were performed using Agilent AreaMx Real-Time PCR System (Agilent Technologies, USA).
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Microbiology 2020Quote: ... ACE2-Fc variants were generated by the QuikChange II site-directed mutagenesis protocol (Agilent).
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bioRxiv - Microbiology 2024Quote: The library of the extracted genomic DNA was prepared by Illumina Nextera XT DNA Library Prep Kit protocol (# FC-131-1096) and analyzed by Agilent D1000 ScreenTape ...
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bioRxiv - Cell Biology 2022Quote: ... HAP1 cells and SH-SY5Y cells were seeded at a density of 40,000 cells/well and HEK293 cells were seeded at a density of 20,000 cells/well on Seahorse XF96 V3-PS Microplates (Agilent Technologies, 101085-004) after being trypsinized and counted (Bio-Rad TC20 automated Cell Counter ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Immunology 2020Quote: ... The DNA sequences encoding the SCP-tags were added by QuikChange site-directed mutagenesis (Stratagene, USA) using pMMHa BPTI-19A vector as a template [23] ...
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bioRxiv - Immunology 2023Quote: ... C5AP and PRGA with streptavidin tag were immobilized on pre-equilibrated AssayMAP Streptavidin columns (Agilent Technologies). Columns were washed with 1XPBS and then 100 µl of IVIG (1µl of IVIG diluted with 1XPBS to a final volume of 100 µl ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Microbiology 2019Quote: All mutated MERS-CoV S constructs were generated by site directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent). pDNA3.1+/MERS-CoV S EMC/2012 served as template for all the mutagenesis performed ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Physiology 2020Quote: ... blocked in 20% fetal calf serum (FCS) and incubated with anti-insulin (1/10, Dako) in Antibody Diluent for 2h ...
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bioRxiv - Genomics 2019Quote: We analyzed somatic mutations in 1642 miRNA gene regions (Supplementary Table S1) including 1600 regions covered by the probes used for exome enrichment (SureSelect, Agilent, Santa Clara, CA, USA) and 42 regions co-captured during library enrichment but not directly covered by the SureSelect probes ...
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bioRxiv - Plant Biology 2020Quote: ... a 6x Histidine tag was appended to the C-terminus of rbcS via the Quikchange protocol (Stratagene). Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen) ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... PARLSkd3 and variants were expressed with an N-terminal MBP-tag in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in lysis buffer (40 mM HEPES-KOH pH = 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... ScFv-Fc were revealed thanks to a polyclonal α-human IgG HRP-conjugated Ab (P0214, Dako), diluted 1:10000 ...
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bioRxiv - Microbiology 2019Quote: ... GFAP (Dako #M076101-2 or #Z033429-2), OLIG2 (R&D Systems #AF2418 ...
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bioRxiv - Cancer Biology 2022Quote: A library of the entire genomic sequence of all 50 known genes (electronic supplementary Table S1) was prepared using HaloPlex Target Enrichment kits (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... 34 plasmids encoding new quantification tag (LVXXLTK) were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies) using appropriate DNA primers (Table S7) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Immunology 2021Quote: QuikChange Lightening Site-Directed Mutagenesis kit was used to generate amino acid substitutions in the SARS-CoV-2 Wuhan Spike expression vector (Seow et al., 2020) or the D614G pCDNA Spike plasmid (S. A. Kemp et al., 2020) following the manufacturer’s instructions (Agilent Technologies, Inc., Santa Clara, CA). Spike B.1.1.7 was synthesised by Genewiz ...
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bioRxiv - Physiology 2020Quote: ... Slides were blocked with 20% FCS in PBS and incubated overnight with 1/10 anti-insulin (Dako) and 1/500 anti-Ki67 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Plant Biology 2019Quote: ... Extracts were derivatized using an AccQ•Tag Ultra Derivatization Kit (Waters Corp.) according to the manufacturer’s protocol and analyzed using an HPLC (Agilent) coupled to a mass spectrometer (4500 QTRAP (Sciex) ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).