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Citations for Agilent :
201 - 250 of 2534 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... coli (Agilent, 200150). Sequences were validated using sanger sequencing with CLASP1 specific primers (CLASP1-1 FW 5’- GTGCTGCTGAGTATGATAACTTCT- 3’ and CLASP1-1 RV 5’-CTGATGACAGATGCCCCAAC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Agilent technologies), and individual colonies picked ...
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bioRxiv - Physiology 2023Quote: ... coli BL21 (Stratagene). Bacteria were grown at 37° C to A600 >0.6 in LB containing 40 mg/L kanamycin and induced with 0.05 mM isopropyl β-D-thiogalactoside (IPTG ...
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bioRxiv - Biophysics 2023Quote: ... coli strain (Stratagene). The LB medium was supplemented with 50 µg/ml kanamycin ...
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bioRxiv - Biochemistry 2023Quote: ... coli cells (Stratagene) and His6-SUMO-TEV-CDK5RAP244-93 expression was induced with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent Technologies) and purified using glutathione resin (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Constructs encoding the 6His-SUMO tagged proteins were transformed into chemically competent BL21(DE3) Escherichia coli (Agilent). A fresh bacteria colony was inoculated to 10 ml LB broth containing 200 μg/ml Ampicillin and 50 μg/ml Chloramphenicol and grown at 37°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 RBD constructs carrying point mutation were generated by following the standard protocol from QuikChange® II XL Kit (Agilent). The cloned genes were sequenced to confirm that no errors had accumulated during the PCR process ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies, Santa Clara, CA) as described 48 and dialyzed against PLS buffer ...
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bioRxiv - Microbiology 2020Quote: ... coli Arctic Express (Agilent). Cells were grown to OD600 = 0.4-0.7 ...
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bioRxiv - Biochemistry 2020Quote: ... coli XL1-blue (Agilent) and E ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21(DE3) (Agilent) for expression and grown overnight at 37°C in 30 ml LB supplemented with 100 μg/ml ampicillin ...
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bioRxiv - Neuroscience 2021Quote: ... coli XL1-blue (Stratagene) and the sequence confirmed ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21 (DE3) (Stratagene) strain ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Agilent Technologies) for expression of Hel66 fused to an N-terminal GST-tag.
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bioRxiv - Biochemistry 2020Quote: ... coli XL1-blue (Stratagene) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Escherichia coli DH5α (Stratagene) was used for standard cloning procedures ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 gold (Stratagene), bacterial lysis with lysozyme ...
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bioRxiv - Cancer Biology 2020Quote: ... coli BL21 (DE3) (Stratagene) and SG13009 (S9 ...
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bioRxiv - Immunology 2021Quote: ... coli cells (Agilent Technologies) by electroporation as before and pipetted on to selective LB agar plates (100 μg mL−1 Ampicillin ...
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bioRxiv - Immunology 2021Quote: ... coli cells (Agilent Technologies) in another study and stored in glycerol at −80 °C (16) ...
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bioRxiv - Cell Biology 2022Quote: ... coli bacterial strain (Agilent). Expression was induced for 3h at 37°C and purified as described (Lata et al. ...
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bioRxiv - Neuroscience 2022Quote: ... coli (230245, Agilent Technologies). A single colony from this transformation was expanded in Terrific Broth (12 g/L of Bacto-tryptone ...
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bioRxiv - Biophysics 2022Quote: ... coli cells (Agilent Technologies) in Lysogeny broth at 37 °C until OD600~0.6-0.8 ...
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bioRxiv - Biochemistry 2022Quote: ... coli XL1Blue MRF’ (Stratagene) at OD600 of 0.5 in 2xYT with 20 μg/ml tetracycline was infected with eluted phages ...
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bioRxiv - Immunology 2022Quote: ... coli TG1 cells (Agilent) were electroporated with the ligation reactions and the obtained ampicillin-resistant colonies were harvested ...
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bioRxiv - Microbiology 2022Quote: ... coli cells (Agilent, 200315) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 cells (Agilent). Cells were grown and protein expression was induced by the addition of 0.2 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Biophysics 2020Quote: ... coli competent cells (Agilent), initially cultured in 1L LB broth at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... coli (Stratagene, CA, USA) on LB-agar ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 (DE3pLys) (Agilent). One plate was used to inoculate 500 ml terrific broth (TB) ...
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bioRxiv - Genetics 2022Quote: ... Coli competent cells (Stratagene), and colonies were selected to verify the presence of the DNM1 fragment ...
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bioRxiv - Immunology 2022Quote: ... coli XL1 blue (Agilent) by electroporation.
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bioRxiv - Molecular Biology 2019Quote: ... coli (Agilent Technologies # 200315) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (Agilent Technologies # 200315) following the manufacturer’s protocol and plasmids were purified using the NuceloBond Xtra Maxi Plus kit (Macherey Nagel # 740416.10 ...
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bioRxiv - Biochemistry 2019Quote: ... coli BL21-DE3 (Stratagene) in LB medium containing 100 μg/ml of ampicillin to a density of A600 = 0.6 ...
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bioRxiv - Immunology 2021Quote: ... coli XL1-Blue (Stratagene) was transformed and plated on LB-agar (containing 1% glucose and 50 μg/ml ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... coli XL1-Blue (Agilent) and 10G (Lucigen ...
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bioRxiv - Immunology 2021Quote: ... coli cells (Agilent Technologies). Bacteria were grown at 37 °C in LB medium to OD600 = 0.6–0.8 and induced with 1 mM isopropyl-β-D-thiogalactoside ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Agilent Technologies) (Qin et al ...
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bioRxiv - Cell Biology 2022Quote: ... coli ArcticExpress cells (Agilent), purified on Cobalt resin ...
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bioRxiv - Genetics 2022Quote: ... coli SURE strain (Stratagene) and the integrity of (GAA)100 repeats was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... coli (Agilent Technologies 200315). After transfection ...
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bioRxiv - Cell Biology 2023Quote: ... coli pLysS cells (Agilent), purified on Talon Cobalt resin ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) (Stratagene). Bacteria were grown at 37 °C in LB medium containing 100 µg/ml ampicillin and protein expression was induced at an OD600 of 0.4 - 0.6 with 0.3 mM isopropylthiol-β-galactoside (IPTG ...
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bioRxiv - Immunology 2023Quote: ... coli TG1 cells (Agilent) were electroporated with the ligation reactions and the obtained ampicillin-resistant colonies were harvested ...