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Citations for Agilent :
351 - 400 of 7006 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... All E protein variants were obtained by site-directed mutagenesis using QuikChange kit (Stratagene, La Jolla, California) and were confirmed by sequencing the plasmid DNA at Macrogen Company (Seoul ...
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bioRxiv - Neuroscience 2020Quote: ... a fixation and perfusion procedure was done as previously described in Cahill et al 2012.22 A multi-channel 7.0T magnet (Agilent Technologies, Santa Clara, CA) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
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bioRxiv - Microbiology 2022Quote: ... The MHC-II binding site mutations were introduced into pKK33 by site-directed mutagenesis (QuickChange II, Agilent Technologies) using the primer set SECF44A/L45Afor/ SECF44A/L45Arev ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Plates were read according to manufacturer’s instructions using the Cytation 3 plate reader (Agilent) with Gen5 software (v2.04).
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bioRxiv - Bioengineering 2020Quote: ... We measured the size distribution and concentration of each well in 96-well plate using Fragment Analyzer High-sensitivity NGS kit (Agilent). We normalized the concentration of cDNA of each well to a concentration of 0.4 ng/µL ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2021Quote: Mutagenesis of the sequence encoding Sir3464-728 using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent, 200552-5) was performed by PCR on 9,5 µg of pACT2-SIR3464-728 with 20 cycles of amplification to allow low mutation rate ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-μm sections were stained with antibodies or control IgG using the EnVision+System-HRP (DAB) kit (Dako North America Inc ...
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bioRxiv - Physiology 2021Quote: ... coated V7 Seahorse plates (Agilent Technologies LDA UK Ltd ...
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bioRxiv - Neuroscience 2023Quote: Seahorse plates (Agilent #101085-004) were precoated with Matrigel solution and left to solidify for one hour at room temperature in cell culture hood ...
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bioRxiv - Physiology 2023Quote: ... a Seahorse sensor plate (Agilent) was calibrated overnight in a non-CO2 incubator ...
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bioRxiv - Immunology 2023Quote: ... 96-well Seahorse plates (Agilent) were coated with CellTak (Corning) ...
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bioRxiv - Immunology 2023Quote: ... 96-well Seahorse plates (Agilent) were coated with CellTak (Corning) ...
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bioRxiv - Cell Biology 2024Quote: ... plates (cat no. 300601140; Agilent) were coated with VCAM-1 (1 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Samples from nine FUSΔ14/Δ14 mice of 10-12 weeks of age and nine age-matched wild type littermates were scanned in a multi-channel 7.0 tesla MRI scanner (Agilent Inc., Palo Alto, CA) using a custom-built 16-coil solenoid array (36) ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding the ACR-16 TM3-4 loop or the SDN-1 intracellular domain full length or lacking the EYFA PDZ binding motif were cloned to pGEX-3X expression vector and then transfected into Arctic express E.coli strain (Agilent). Bacterial clones were first cultured overnight in 5 mL LB medium supplemented with 100 mg/mL ampicillin ...
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bioRxiv - Pathology 2023Quote: ... Specific antibody binding was detected using a second antibody and the LSAB system (Dako Corporation, Carpinteria CA, USA, K690) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µm (Agilent). Next ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... the fluorescence intensity of the samples and blanks was measured (λex = 495 nm and λem = 529 nm) using a SynergyTM H1 multi-mode microplate reader (Agilent, Santa Clara, CA, USA). Each sample was measured in duplicate ...
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The Tah1 mutation in the MEEVD binding site was achieved by PCR-based site-directed mutagenesis (Quick-Change method, Stratagene) using suitable primer sequences with the desired mutation ...
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bioRxiv - Immunology 2023Quote: ... in the α3 domain of HLA-A*02:01 described to abrogate binding of CD8 (13) were changed by site-directed mutagenesis (Agilent).
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue was permeabolized with 0,5% Triton X-100 in PBS (PBS-T) and non-specific binding was blocked by incubating the tissue section with blocking reagent (DAKO). Antibody staining was performed in PBS-T with 1% blocking reagent (DAKO ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Microbiology 2022Quote: ... RTCA E-plate VIEW 16 plates with embedded golden electrodes (#300600880, Agilent, Santa Clara, CA, USA) were used for the experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA concentrations were determined with Synergy H1 plate reader equipped with Take3 Plate (BioTek, Agilent). Quality control analysis was performed with an automated 4200 TapeStation System (Agilent ...
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bioRxiv - Cell Biology 2019Quote: ... Seahorse XF96 plates were from Agilent® technologies (Little Island ...
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bioRxiv - Immunology 2020Quote: ... Seahorse XF96 plate (101085-004, Agilent) was used following the manufacturer’s instructions for XF Cell Mito Stress Test Kit (103015-100 ...
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bioRxiv - Cell Biology 2022Quote: ... An equilibrated Seahorse cartridge plate (Agilent) was loaded with 20 mM ADP ...
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bioRxiv - Cell Biology 2019Quote: Cell mitochondrial oxygen consumption rates (OCRs) were assayed in 96-well plates by using a XF Cell Mito Stress Test Kit (Cat. #: 101706100, Seahorse Bioscience, U.S.) on the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience ...
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bioRxiv - Neuroscience 2020Quote: ... multi-site directed mutagenesis was performed by using the GA7546G:luc2 construct with rs221962608 SNP variant as the template (Agilent, Santa Clara, CA, USA; Cat# 200514). The mutagenic primers are provided in Supplemental Table 2.
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Microbiology 2021Quote: ... a BamHI site present in the 5’-UTR was first mutated to ggctcc using the QuickChange Site-Directed Mutagenesis Kit (Stratagene) and primers P7/P8 ...
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bioRxiv - Cell Biology 2020Quote: ... were changed to 5′-ACCTGCAAAG −3′ by using the Quick-Change site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). Expression vectors for SENP2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality of final single cell 5’ GEX libraries was assessed by Qubit quantification and fragment analysis (DNF-474 High Sensitivity NGS Fragment Analyzer Kit, Agilent). The sequencing was performed on a NextSeq500 device (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... pCS2+-5’HA-sobp and pCS2+-sobp-3’HA were generated using the QuikChange lightning Site-directed mutagenesis kit (Agilent). The same kit was used to sequentially remove three nucleotides located at the 5’ end of the pCS2+-3’HA-sobp ORF to generate a construct whose transcribed mRNA does not bind to the designed translation-blocking antisense morpholino oligonucleotide (pCS2+-sobpMOI-3’HA ...
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bioRxiv - Microbiology 2020Quote: ... and ZIKV X1 C35G R (5’-GCCTGCTAGTCAGGCACAGCTTGGGGA-3’) were used with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) to introduce the X1 C10415G mutation into the p2-ZIKV plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Mutagenesis of the A2REs in the Ins1 5’-UTR was performed with the QuikChange II Site-directed mutagenesis kit (Agilent). Cells were co-transfected with each pNL1.1 construct and the pGL4.54 for normalization ...
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bioRxiv - Molecular Biology 2022Quote: ... USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A) using the sense primer (5’-gtttgcaaaaggctattcaaataggcagtcattgcttggtttactagtcc-3’) using Quickchange Lightning Site-directed Mutagenesis Kit from Agilent technologies ...