Labshake search
Citations for Agilent :
51 - 100 of 627 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
-
bioRxiv - Molecular Biology 2022Quote: Recombinant production/purification: The MLucV construct was expressed in BL21-CodonPlus (DE3)-RIPL strain (Agilent) using ampicillin and chloramphenicol as the selection antibiotic ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated recombinant NS1 was purified by FPLC and then tetramerized to fluorochrome-conjugated streptavidin (Prozyme). To detect NS1-specific B cells ...
-
bioRxiv - Biophysics 2023Quote: Recombinant Pil1 and Pil1-mCherry were expressed in BL21(DE3)pLysS (#200132, Agilent Technologies Inc.) in auto induction LB medium (#AIMLB0210 ...
-
bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
-
bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
-
bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
-
bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
-
bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
-
bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
-
bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
-
bioRxiv - Cancer Biology 2020Quote: ... dry protein A cartridges (Agilent) were primed in PBS at 300 μL/min before loading 1 mg of antibody at 1 mg/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
-
bioRxiv - Cancer Biology 2023Quote: ... blocked in Protein block (DAKO) for 10min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... BSA and Protein block (Dako). Sections were stained with primary antibodies against GFP (ab290 ...
-
bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein Block Serum Free reagent (Dako). Primary antibody incubation was performed at 4°C overnight using the ideal dilution for each antibody (Supplementary Table 5) ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated in Protein Block (Dako) for 5 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each ...
-
bioRxiv - Microbiology 2023Quote: ... AdvanceBio SEC 300Å protein standard (Agilent) was included to correlate the elution volume with molecular weight.
-
bioRxiv - Microbiology 2023Quote: ... diluted with protein block buffer (Dako) for 2 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2) ...
-
bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
-
bioRxiv - Immunology 2020Quote: ... The recombinant Ad5 genomes with HA inserts were linearized with PacI and buffer exchanged using a Strataprep PCR purification kit (Agilent Technologies). The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen) ...
-
bioRxiv - Biophysics 2019Quote: Human myosin IIa S1 and lever-arm-less S1: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies) ...
-
bioRxiv - Genetics 2024Quote: Recombinant adenoviruses expressing mouse SAR1A or SAR1B were constructed using pAdTrack-CMV and the AdEasy adenoviral vector system (Agilent Technologies, Lexington MA). Adenoviruses were amplified in Ad293 cells and purified using CsCl gradient ultracentrifugation ...
-
bioRxiv - Immunology 2020Quote: ... proteins were tetramerized with streptavidin-APC (Prozyme) or streptavidin-AlexaFluor488 (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were tetramerized with streptavidin-APC (Prozyme), streptavidin–Alexa Fluor 488 (Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Additional protein block from Dako (X090930-2) was applied ...
-
bioRxiv - Neuroscience 2023Quote: ... blocked (30 min, protein block X0909, Dako), and stained overnight at 4°C using primary antibodies detecting F4/80 ...
-
bioRxiv - Immunology 2023Quote: ... using the Protein G AssayMAP Bravo (Agilent) system ...
-
bioRxiv - Immunology 2023Quote: ... using the Protein G AssayMAP Bravo (Agilent) system ...
-
bioRxiv - Cell Biology 2021Quote: ... The recombinant transfer vector was linearized by PmeI and transformed into electro-competent E.coli strain BJ5183-AD-1 (Stratagene, Cat. No. 200157-11) for in vivo recombination with pAdEasy vector ...
-
bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
-
bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
-
bioRxiv - Cancer Biology 2021Quote: ... and an additional serum-free protein block (DakoCytomation) to reduce nonspecific binding of endogenous proteins ...
-
bioRxiv - Synthetic Biology 2019Quote: ... After blocking with serum-free protein block (Dako), they were incubated with the primary antibodies for 120 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... rabbit anti-glial acidic fibrillary protein (GFAP)(Dako), mouse anti-glutamic acid decarboxylase 67 (GAD67 ...
-
bioRxiv - Bioengineering 2020Quote: Proteins were expressed in BL21(DE3)Gold (Stratagene) and purified as described previously6 ...
-
bioRxiv - Biophysics 2021Quote: ... proteins were expressed using BL21(DE3) cells (Agilent). The cells were grown at 37°C until an optical density at 600nm of .6 then induced with 0.2mM isopropyl-Beta-D-thiogalactoside overnight at 18° C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein blocking was performed for 20 min (Dako). NUP210 primary antibody (Atlas ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein was overexpressed in BL21(DE3) RIL (Agilent) at 37 °C in Terrific Broth (TB ...
-
bioRxiv - Pathology 2021Quote: ... Slides were placed in a Protein Block (DAKO) for 10 minutes ...