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Citations for Agilent :
301 - 350 of 1000 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Washing procedure was repeated and cells were coversliped using an aqueous fluorescent mounting medium (Dako). The specimens were analysed under a confocal microscope Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed with DPBS and cover-slipped with fluorescent mounting medium (#S3023, Dako). The stained tissues were analyzed with a TCS SP8 DMI laser confocal scanning microscope (Leica Microsystems ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by the slides being mounted in Dako Fluorescent Mounting Medium (Agilent, Santa Clara, CA). Images were taken with an Axio Imager M2 microscope (Zeiss) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was labelled with Cy3 (ULS™ Fluorescent Labeling Kit for Agilent Arrays (EA-023), Kreatech ...
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bioRxiv - Neuroscience 2022Quote: ... All slices were subsequently mounted onto glass slides with DAKO fluorescent mounting medium (Agilent Technologies). Images of each section were acquired using a confocal microscope (Zeiss LSM800 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10min and then dried and covered DAKO fluorescent mounting medium (Agilent, Santa Clara, CA) and a coverslip prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were washed three times in 1x PBS before mounting in Fluorescent Mounting Medium (DAKO).
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted on glass slides using Dako fluorescent mounting medium (Dako Inc, Carpinteria, USA).
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in PBS tween and mounted using fluorescent mounting medium (Agilent Dako, #S3023) for imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in PBS tween and mounted using fluorescent mounting medium (Agilent Dako, #S3023) for imaging ...
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bioRxiv - Immunology 2023Quote: ... and mounted on microscope slides with fluorescent mounting media (Dako North America, Carpinteria, CA, USA). Ten view fields (1×1 mm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were counterstained with DAPI and the slides were mounted in fluorescent mounting medium (Dako). Images were captured using Axioimager with an M2 microscope (Carl Zeiss ...
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bioRxiv - Molecular Biology 2024Quote: ... The fluorescent signal was analyzed using the BioTek Gen5 software (Agilent Technologies, La Jolla, CA), and normalized by the total protein content of the sample.
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bioRxiv - Neuroscience 2024Quote: ... sections were mounted on gelatin-coated slides with DakoCytomation Fluorescent Mounting Medium (DakoCytomation, Carpinteria, CA). To enhance and facilitate the visualization of the GFP signal from transgenic mice ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Red fluorescence was quantified 48 hours after transfection using the Cytation3 (Agilent-Biotek Inc.) platform and subsequently after antibiotic selection ...
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bioRxiv - Neuroscience 2021Quote: ... Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO, K0597) for 30 minutes.
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bioRxiv - Cell Biology 2022Quote: ... The FLAG-tagged version of Cp was created by sequential mutagenesis reactions using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Neuroscience 2021Quote: The GFP tagged FUS P525L variant was created by site directed mutagenesis on the GFP tagged FUS WT construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent; 200521). The primers used to generate the construct were ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Physiology 2020Quote: ... 20 μL samples were injected into Hi-Plex H column (300×7.7 mm; 8 μm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
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bioRxiv - Biophysics 2020Quote: ... Amino acids were then converted into respective fluorescent derivatives using o-phthalaldehyde (OPA) (Agilent #5061-3335). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... After 3 final washing steps sections were mounted with Dako fluorescent mounting medium (Agilent Technologies, USA) and sealed with nail polish ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were mounted on glass slides and cover slipped using fluorescent mounting medium (Dako S3023).
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bioRxiv - Neuroscience 2019Quote: ... was used to stain the nucleus before cells were mounted using DAKO fluorescent mounting medium (DAKO). Neurons were fixed at DIV 21 for synaptic stainings ...
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bioRxiv - Cancer Biology 2022Quote: ... All sections were counterstained with nuclear DAPI (1:1000) and mounted with fluorescent mounting medium (Dako).
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bioRxiv - Neuroscience 2022Quote: ... the sections were mounted on slides with Dako fluorescent mounting medium (Agilent-a o S3 3). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2023Quote: ... and the slices were then mounted on microscope slides with a fluorescent mounting medium (Dako, Germany). 3D image stacks were obtained on an epi-fluorescence microscope (Axio Imager.M2 with ApoTome.2 ...
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bioRxiv - Neuroscience 2023Quote: ... and the slices were then mounted on microscope slides with DAKO fluorescent mounting medium (DAKO, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent images were obtained on a Keyence BZ-X710 microscope or on a Cytation 7 (Agilent).
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Microbiology 2021Quote: ... A bright red chromogen labelling was produced with 3-amino-9-ethylcarbazole substrate (AEC, DAKO). Sections were counterstained with Mayer’s haematoxylin ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Microbiology 2020Quote: ... the samples were mounted on slides using Dako Cytomation fluorescent mounting medium (Dako North America, Inc., USA). All samples were observed using a Zeiss LSM 700 laser scanning confocal microscope (Zeiss ...