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Citations for Agilent :
101 - 150 of 217 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
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bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
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bioRxiv - Microbiology 2019Quote: ... All GBF1 truncations were introduced into the backbone of Venus tagged-GBF1/A795E (described in 57) using QuickChange XL site-directed mutagenesis kit from Agilent Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: Genes encoding rat Kir6.2Q52R and N-terminal FLAG-tagged (DYKDDDDK) hamster SUR1 were cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant production/purification: The MLucV construct was expressed in BL21-CodonPlus (DE3)-RIPL strain (Agilent) using ampicillin and chloramphenicol as the selection antibiotic ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated recombinant NS1 was purified by FPLC and then tetramerized to fluorochrome-conjugated streptavidin (Prozyme). To detect NS1-specific B cells ...
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bioRxiv - Biophysics 2023Quote: Recombinant Pil1 and Pil1-mCherry were expressed in BL21(DE3)pLysS (#200132, Agilent Technologies Inc.) in auto induction LB medium (#AIMLB0210 ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid for production of (His)6-SUMO-σAntA-DD was generated by site-directed mutagenesis (Agilent QuikChange) using primers listed in Table S3 ...
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bioRxiv - Cancer Biology 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). An equimolar pool of the different samples was sequenced on the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Genomics 2023Quote: ... Size and concentration of the finished Hi-C libraries was assessed by DNA ScreenTape Analysis (Agilent #5067-5582) on an Agilent 2200 Tapestation.
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bioRxiv - Genomics 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). Four biological replicates have been pooled in an equimolar manner and subjected to sequencing using the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNAs were obtained and amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The FLAG-tagged version of Cp was created by sequential mutagenesis reactions using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Neuroscience 2021Quote: The GFP tagged FUS P525L variant was created by site directed mutagenesis on the GFP tagged FUS WT construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent; 200521). The primers used to generate the construct were ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Neuroscience 2023Quote: Primary rat neurons or human i3Neurons were plated on Seahorse XFp plates (Agilent) at a density of 40,000 cells/well ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Physiology 2020Quote: ... 20 μL samples were injected into Hi-Plex H column (300×7.7 mm; 8 μm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...