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Citations for Agilent :
51 - 100 of 5634 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... anti-mouse-IgG conjugated to horseradish peroxidase (1:10,000, cat. no. P016102-2; Dako, Carpinteria, CA, USA), anti-rabbit StarBright 700 (1:2500 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with primary antibodies CD31 (1:200, M082329-2, DAKO), NG2 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were rabbit (P044801-2) or mouse (P044701-2) HRP-conjugated (Dako, Agilent), used at 1:10000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were rabbit (P044801-2) or mouse (P044701-2) HRP-conjugated (Dako, Agilent), used at 1:10000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture of early/late apoptosis and some necrotic death was induced by irradiating Jurkat cells and the isolated neutrophils under a UV lamp (254nm, Stratalinker UV 1800, Stratagene Corporation, La Jolla, CA) for 1 and 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and mouse monoclonal anti-α-SMA (M085129-2, Dako), Secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-mouse IgG HRP (Agilent technologies/P044701-2), goat anti-rat IgG HRP (Abcam/ab57057 ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated anti-mouse Ig (Agilent Cat# K400111-2), MACH4 universal HRP-Polymer (Biocare Medical Cat#M4U534L) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with polyclonal goat anti-mouse FITC (DAKO F0479 1:20). Finally ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were then incubated at 37°C for 48 hours then analyzed by flow cytometry and ELISA or monitored for target cell death using xCelligence eSight (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2022Quote: ... TAP-tagged proteins were detected using rabbit antiperoxidase antibody linked to peroxidase (PAP, Dako; 1:10000 dilution). Tubulin was detected using mouse-anti-α-tubulin antibody (Sigma-Aldrich T5168 ...
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bioRxiv - Biophysics 2020Quote: ... The recombinant construct was transformed into Arctic express (DE3) competent cells (Agilent Technologies, USA). The transformed cells were grown at 37°C in LB media supplemented with 100 μg/ml ampicillin and 10 μg/ml gentamycin ...
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies were HRP-coupled: anti-mouse (P026002-2, Dako), anti-rabbit (A16029 ...
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bioRxiv - Biochemistry 2021Quote: ... 2) biotinylated goat anti-mouse Ig antibody (Dako, Glostrup, Denmark), diluted 1:5000 with TBS-BSA ...
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bioRxiv - Microbiology 2021Quote: ... and CD68 (clone KP1) mouse monoclonal (Agilent cat#M081401-2). Secondary antibodies include Discovery Red 610 (Roche Tissue Diagnostics cat#760-245) ...
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bioRxiv - Cancer Biology 2023Quote: ... or rabbit anti-mouse IgG HRP (Agilent technologies, P044701-2) at 1:5000 ...
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bioRxiv - Biophysics 2022Quote: A plasmid encoding 6xHis-tagged methionyl-tRNA synthetase (MetRS) was transformed into BL21 (DE3) Codon+ RIL cells (Agilent). Overnight cultures were diluted (1:100 ...
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bioRxiv - Biophysics 2022Quote: Octahistidine-tagged Sec14 (His8-Sec14) was purified from BL21-CodonPlus (DE3)-RIL cells (Stratagene, La Jolla, CA, USA) as described for Sfh1 (Schaaf et al. ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... CD31 (1:100, mouse, Dako), Podocalyxin (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... NSE (Dako, mouse, 1:1,000), GFAP (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies tagged with Horse Radish Peroxidase (HRP; Dako) were incubated for 1 h at room temperature under rotation ...
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bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Ki67 (Dako, MIB-1, 1:100), mouse anti-DLX2 (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies (HRP linked) were swine anti–rabbit (P039901-2) and goat anti–mouse (P044701-2) (DAKO). Imaging was done using SuperSignal West Femto (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... encoding N-terminally 6xHis tagged MBP-MS2 (Gift from Nadège Liaud, UC-Berkeley) was transformed into BL21 Codon+ RIL chemically competent cells (Agilent). An overnight culture was used to inoculate 3L of ZYM-5052 auto-inducing media (Studier ...
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bioRxiv - Developmental Biology 2023Quote: ... Insulin (1:2, Dako IR002), Integrin-α6 (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated at room temperature for 1 h with rabbit anti-mouse IgG (Agilent, P026002-2) or goat anti-rabbit IgG (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... or EnVision+ HRP anti-mouse (Dako, Carpinteria, CA; catalog #K400111-2) secondary antibodies and developed using DAKO DAB ...
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bioRxiv - Molecular Biology 2020Quote: ... and rabbit anti-mouse HRP-conjugated secondary antibody (P026002-2, Dako). Antibodies used for SBA ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies included a mouse monoclonal anti-Ki67 (DAKO, Clone IVAK-2), 1:100 dilution) ...
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bioRxiv - Immunology 2024Quote: ... Cells (1-2 × 105) were plated in Poly-D-Lysine coated 96-well microplates (Agilent) with 4-5 technical replicates ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-DESMIN (1:200; Dako), rat anti-F4/80 (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... or mouse (Dako, M7240, 1:50) anti-Ki67 monoclonal antibody ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse (goat, Dako, 1:2000), anti-rat (rabbit ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse anti-Ki67 (Dako, 1:500), rabbit anti-GFAP (Dako ...