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Citations for Agilent :
1 - 50 of 1932 citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... anti-ERBB2 antibody (Dako, A0485), or anti-S100A1antibody (Dako ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were made by conjugating the biotin-labeled monomers with PE-labeled or APC-labeled streptavidin (Agilent) according to protocols from the NIH tetramer facility ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-ErbB2 rabbit pAb (IF: 1:100; A0485, Agilent), anti-ErbB3 mouse mAb (IF ...
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bioRxiv - Immunology 2020Quote: ... proteins were tetramerized with streptavidin-APC (Prozyme) or streptavidin-AlexaFluor488 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Proteins were tetramerized with streptavidin-APC (Prozyme), streptavidin–Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by secondary antibody Envision+ anti-mouse labeled-polymer (Dako/Agilent). Slides were counterstained using Gill’s I hematoxylin (Richard-Allen) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by secondary antibody Envision+ anti-mouse labeled-polymer (Dako/Agilent). Slides were counterstained using Gill’s I hematoxylin (Richard-Allen) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by anti-rabbit or anti-mouse peroxidase-labeled polymer (Dako). Sections were developed using 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Cell Biology 2021Quote: Constructs encoding the 6His-SUMO tagged proteins were transformed into chemically competent BL21(DE3) Escherichia coli (Agilent). A fresh bacteria colony was inoculated to 10 ml LB broth containing 200 μg/ml Ampicillin and 50 μg/ml Chloramphenicol and grown at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... TAP-tagged proteins were detected using rabbit antiperoxidase antibody linked to peroxidase (PAP, Dako; 1:10000 dilution). Tubulin was detected using mouse-anti-α-tubulin antibody (Sigma-Aldrich T5168 ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... BrdU was immunofluorescently labeled with a mouse anti-BrdU antibody (DAKO, clone Bu20a) and a fluorescein-conjugated donkey anti-mouse antibody (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... EnVision+ system-HRP-labeled polymer anti-mouse secondary antibody (Dako, Carpinteria, CA, USA) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Immunology 2023Quote: ... or APC (Prozyme/Agilent) streptavidin at a 4.5:1 ratio (59) ...
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bioRxiv - Immunology 2023Quote: ... or APC (Prozyme/Agilent) streptavidin at a 4.5:1 ratio (59) ...
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bioRxiv - Immunology 2022Quote: ... streptavidin-APC (PJ27S, ProZyme), streptavidin-BV421 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... or streptavidin-APC (Agilent) as previously described23,74 ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1-hour incubation with peroxidase-labeled goat anti-mouse antibodies (1:1000; DAKO) and a 7 min incubation with the True Blue™ (KPL ...
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bioRxiv - Microbiology 2021Quote: Immunostaining was detected by EnVision system peroxidase-labeled anti-rabbit or anti-mouse immunoglobulin (DAKO) and visualized with a Histofine diaminobenzidine substrate kit (Nichirei Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... The labeled RNAs were hybridized to a Mouse miRNA Microarray (G4472B, Agilent Technologies, Basel, Switzerland) for 20 hours at 55°C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... The labeled proteins were subjected to digestion with Sialidase A (Agilent Technologies, Santa Clara, CA, USA), β1-4 galactosidase (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Product titer was analyzed offline from spent media daily by protein A chromatography on the Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA). An XCell™ ATF system (Repligen ...
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bioRxiv - Biochemistry 2023Quote: Titer analysis of spent medium was analyzed offline daily by Protein A chromatography on an Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA) as well as with the BLItz biolayer interferometer system (ForteBio ...
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bioRxiv - Immunology 2022Quote: ... or streptavidin-APC (Prozyme PJ27S) for 30 min at 4°C ...