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Citations for Agilent :
101 - 150 of 1202 citations for Recombinant Mouse Dll4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit/Mouse (Agilent). This staining procedure can be used for sequential double staining of two different antigens with primary antibodies raised in the same species by incorporating a blocking step between the first and second antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit/mouse (Dako) was used to visualize the antibody staining ...
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bioRxiv - Immunology 2024Quote: ... Rabbit/Mouse (Agilent) and counterstained with hematoxylin (Wako) ...
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... and GST or His6-MBP-tagged DI-DAX variants were transformed into BL21(DE3) Codon-Plus RIL Escherichia coli (Agilent, Santa Clara CA). A single colony or a scraping from a glycerol stock was used to inoculate a starter culture ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was purified from total RNA using biotin-tagged poly dT oligonucleotides and streptavidin-coated magnetic beads and quality was assessed using an Agilent Technologies 2100 Bioanalyzer (Agilent, Santa Clara, CA).
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bioRxiv - Genomics 2020Quote: ... HindIII-based Hi-C libraries from our previous study (Wutz et al., 2017) were captured using SureSelect target enrichment system (Agilent Technologies) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were subjected to C18 desalting using an Agilent 1260 Infinity LC system equipped with an MRP-C18 Hi-recovery column (Agilent, USA) before SpeedVac drying.
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bioRxiv - Microbiology 2022Quote: ... and analysed by HPLC Shimadzu, Prominence-I (Milton Keynes, UK) with an ion exchange column Hi PlexH (300 × 7.7 mm) (Agilent, Cheadle, UK) and 0.6 mL min-1 flow rate at 55 ºC (oven temperature ...
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: M280 anti-mouse magnetic beads were coupled to control mouse IgG2a (Dako) or mouse monoclonal anti-Ku antibodies (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse-HRP (Dako) secondary Abs or StrepTactin-HRP (IBA-LifeSciences ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-hCK18 (Agilent, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: ... -goat anti-mouse (Dako).
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bioRxiv - Molecular Biology 2022Quote: ... Rabbit/Mouse (Dako, Denmark).
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bioRxiv - Developmental Biology 2023Quote: ... p63 (mouse, DAKO, M7317). Sections were extensively washed in 1X PBS and incubated with secondary antibodies (Alexa Fluor 488 anti-rabbit A11008 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse (Agilent #P0260) or anti-rabbit (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse envision (K4001, Agilent) diluted 1:3 with PBS was used for detection of IgG ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...