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Citations for Agilent :
251 - 300 of 727 citations for Recombinant Human PRMT5 Flag & His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit anti-human tau was from Dako (1:8000, A0024). Other tau antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) for human CD45 antibodies (IR75161-2, Agilent Technologies) was performed on FFPE blocks of engrafted tumors to identify cases of lymphomagenesis ...
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bioRxiv - Immunology 2022Quote: ... the plates were incubated with either anti-human-HRP (Dako), anti-rat-HRP (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... (30) using the SureSelectXT Human All Exon 50 Mb (Agilent) bait set ...
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bioRxiv - Cancer Biology 2020Quote: ... monoclonal mouse anti-human alpha smooth muscle actin (M0851, Dako), monoclonal mouse anti-human CD68 (M0814 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1:400 diluted Rabbit anti-human calcitonin (Dako, Denmark) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were analyzed against the Stratagene Universal Human Reference (Agilent). Raw fluorescence intensities were quantified and normalized (Lowess normalization ...
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bioRxiv - Cell Biology 2021Quote: ... IgA heavy chain (rabbit anti-human, Dako, A0262 1:1000) and secretory component/pIgR (goat anti-human ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-SMA (1:67, human, Dako, M0851, Santa Clara, CA) with secondary donkey anti-mouse Cy3 (1:200 ...
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bioRxiv - Immunology 2020Quote: ... 0.006 g/L polyclonal rabbit anti-human CD3 antibody (Dako A0452 ...
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bioRxiv - Cancer Biology 2020Quote: ... whole Human Genome 44 K arrays (Agilent Technologies, product G4112A) were used for stroma and epithelial expression profiles ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako, Santa Clara ...
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bioRxiv - Genetics 2022Quote: ... including A sample (Universal Human Reference RNA, Agilent Technologies, Inc.) and B sample (Human Brain Reference RNA ...
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bioRxiv - Immunology 2023Quote: ... stimulation with rabbit anti-human IgE (Dako, Carpinteria, CA, USA) and fMLP (N-Formylmethionine-leucyl-phenylalanine ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-human CD68 (Dako #M0814, 1:400 dilution), following by the secondary antibody incubation and chromogenic detection with DISCOVERY Purple and Yellow kits (Ventana-Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... The Agilent SureSelect Human All Exon v6 kit (Agilent Technologies) was used for whole exome sequencing ...
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bioRxiv - Genetics 2024Quote: ... The exomes of the subjects were captured by Agilent SureSelect Human All Exon V6 Enrichment kits (Agilent, CA, USA) and then sequenced on a HiSeq X-TEN system (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Biochemistry 2020Quote: All mutations in C100 FLAG were introduced by site-directed mutagenesis (QuickChange Lightning Site Directed Mutagenesis kit, Agilent) in pET 22b vector ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... and p-dsbS(S239A)- flag were obtained using the QuikChange II site-directed mutagenesis kit (Stratagene, catalog#: 200518). For generating p-dsbR(D51A)-flag ...
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bioRxiv - Cell Biology 2023Quote: ... an asparagine at position 1326 of hsNUDT9H-FLAG (DsRed) was replaced with aspartate using QuikChange mutagenesis system (Agilent). Defined oligonucleotides in sense and antisense configuration were obtained from (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: ... containing an AgeI restriction site in the FLAG sequence created by site-directed mutagenesis (QuikChange Mutagenesis kit, Agilent) using oligonucleotides detailed in Reagents and Tools table.
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies, Santa Clara, CA, USA) using the Neon Transfection System (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The EXD2-M126A variant was created by site-directed mutagenesis of pDEST-EXD2_iso1-BirA*-FLAG construct using Quick Change Lightning kit (Stratagene).
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Immunology 2020Quote: ... The pcDNA3.1 Flag-STING-C206S construct was generated using the Quikchange Lightning Site-Directed Mutagenesis kit from Agilent (#210518) with the following primer (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Neuroscience 2023Quote: EEF1A2 mutations in both the CMV-NGFP-EEF1A2-Neo vector and the pcDNA3.1-CMV-Flag-EEF1A2 vector were generated using the QuikChange II site directed mutagenesis kit (Agilent) according to the manufacturers protocol using the following primers.
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human HLA-DRα mAb (clone, TAL. 1B5; Dako Cytomation), mouse anti-human CD163 mAb (clone ...
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bioRxiv - Cancer Biology 2019Quote: Whole Human Genome Microarray Kit 4×44K (Agilent, Cat. No. G4112F) was used to detect mRNA expression levels in cells transfected with control and miR-101-3p transfected HCT116 cells ...
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bioRxiv - Immunology 2019Quote: ... Serial 10-fold dilutions of mouse or human reference RNA (Agilent) were run in duplicate for each PCR run.
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bioRxiv - Genetics 2020Quote: ... SureSelectXT Human All Exon V6 +UTR (Agilent Technologies, Santa Clara, CA) was then used to perform exome capture and library preparations The library were then sequenced using a NovaSeq 6000 System (Illumina ...
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bioRxiv - Genomics 2020Quote: ... QPCR Universal Human Total Reference RNA (UHR) (Agilent, Cat no: 750500) was included in all batches to assess batch effect.
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bioRxiv - Cancer Biology 2021Quote: ... Exomes were sequenced using the AllExon Human SureSelect v7 Kit (Agilent).
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bioRxiv - Biochemistry 2020Quote: ... using the Multiple Affinity Removal Column Human 14 (MARS 14, Agilent) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Exome fragments were captured using the SureSelect Human Exon V5 (Agilent) and sequenced on the Illumina HiSeq 2500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were incubated with human-specific anti-Ki67 (DAKO, cat# M7240) or human specific anti-ClCaspase3 (Cell Signaling ...
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bioRxiv - Cancer Biology 2019Quote: ... MSH6 (Monoclonal Rabbit Anti-Human; Clone EP49, dilution 1 : 1000 Dako) PMS2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Genetics 2020Quote: ... Enrichment was done with SureSelectXT Human All Exon v6 (Agilent Technologies) on 1µg DNA ...