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Citations for Agilent :
451 - 500 of 667 citations for Recombinant Human KLK4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... the hydrogels were incubated overnight with a mouse anti-human α-smooth muscle actin antibody (DAKO, Glostrup, Denmark) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 90 of human ATG12 were mutagenized into arginine using site-directed mutagenesis following the manufacturer’s instructions (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... target enrichment was performed using the Agilent SureSelect Human All Exon V6 design (Agilent, Santa Clara, CA, USA).
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bioRxiv - Genetics 2019Quote: ... Total human brain RNA was derived from post mortem cortex and provided by Agilent (Agilent Technologies, cat: 540143). For the preparation of mouse brain RNA ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako, M0823, 1:100) and Alexa488-conjugated goat anti-mouse secondary antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: Two-channel microarray experiment was performed with two replicates using the Agilent Whole Human Genome Oligo Microarray (Agilent, catalog #G4851C Whole Human Genome Microarray 8×60K) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA of tumor and matched germline samples were captured using the SureSelect Human All Exon v7 (Agilent) or xGen Exome Research Panel v2 (IDT) ...
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bioRxiv - Biochemistry 2022Quote: Aliquots of 20 µL of human plasma were depleted on a 1260 Infinity Bio-inert LC system (Agilent) coupled to a depletion column (human 14 multiple affinity removal column ...
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bioRxiv - Genomics 2021Quote: ... Exome capture was performed using a SureSelect Human All Exon V6 capture kit (Agilent Technologies, Santa Clara, CA). Samples were sequenced on a HiSeq 4000 next-generation sequencing system (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide microarrays for human whole genome (G4858A design 072363, 8×60k chips SurePrint G3 unrestricted GE, Agilent Technologies) were used for global gene expression analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 2009). MEKK1 542–888 construct (H. sapiens MEKK1 TOG) was amplified from the MegaMan human transcriptome library (Agilent). The TOG construct was cloned into pETNKI-his-3C-LIC-kan vector ...
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bioRxiv - Molecular Biology 2019Quote: ... The R1285Q mutant of human FANCI (FANCImut) was generated by using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), and was cloned with a N-terminal cleavable Flag tag into the pCDNA 3.1 vector ...
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bioRxiv - Genetics 2019Quote: ... and exome DNA was enriched with an Agilent SureS elect Human All Exon V5 kit (Agilent Technologies, USA). Paired-end sequencing was performed on Illumina HiSeq 4000 (Supplementary Table 1 and 2).
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bioRxiv - Physiology 2021Quote: Mutations were introduced into human Cx50 cDNA in a SP64T vector with QuikChange Site-Directed Mutagenesis Kit (Stratagene) by using PCR primers that amplified the whole plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and incubated with horse radish peroxidase (HRP) conjugated goat-anti-human IgG antibody (Dako, P0447) (diluted 1:1,600 in PBS/0/1% BSA ...
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bioRxiv - Genetics 2020Quote: ... The whole exome was captured by an Agilent SureSelect All Human exon v5.0 kit (Agilent, Santa Clara, US.), and sequenced on an Illumina Hiseq X-10 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
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bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Cancer Biology 2023Quote: ... which recognises residues 1978-2016 of human NaV1.5) and stained with EnVision+ Dual Link System/HRP (DAB+; Dako), following the manufacturer’s instructions and counterstained with Mayer’s haematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Quick Amp Labeling Kit and SurePrint G3 Human Gene Expression 8×60Kv3 Microarray (Cat. No. G4851C, Agilent Technologies) was used corresponding to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Positive control PhIP-seq designed to capture mouse and human GFAP began by incubating 0.1µg/mL polyclonal anti-GFAP antibody (Dako) with the murine PhIP-seq library.
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The sequence library was prepared using a Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced using a 2500 Illumina with 125-bp paired-end reads (Illumina ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genomics 2019Quote: ... 37,608 individual biotinylated RNAs targeting the ends of 22,076 promoter-containing human HindIII restriction fragments) were prepared according to the manufacturer’s instructions (SureSelect Target Enrichment, Agilent Technologies). The Hi-C library DNA was denatured for 5 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Genetics 2019Quote: ... DNA libraries were prepared by using the SureSelect Human All Exon V6 kit (Agilent Technologies, Santa Clara, CA, USA) and were paired-end sequenced on the Illumina HiSeq 4000 platform at 100X coverage ...
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bioRxiv - Genetics 2021Quote: Oxford Nanopore MinION long-read sequencing was performed on RNA isolated from an adult human cortex (540005, Agilent Technologies). PolyA-enriched RNA was isolated using the Dynabeads mRNA Direct kit (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome-sequencing libraries were generated using the Agilent SureSelect Human All Exon V4+UTR capture platform (Agilent, CA, USA). Libraries were sequenced to a target depth of 200-fold coverage on a 100 bp paired-end sequencing run using an Illumina HiSeq 2000 instrument ...
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bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...
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bioRxiv - Neuroscience 2021Quote: Oxygen Consumption Rate (OCR) from live human iPSC-derived neurons was measured using a Seahorse XFe-96 Analyzer (Agilent). 30,000 human pre-differentiated neurons were seeded per well in Poly-D-Lysine + Laminin-coated 96-well Seahorse plate and differentiated for the indicated time (2 or 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: The tumor and normal adjacent samples were prepared using SureSelect Human All Exon V7 library (Agilent, Santa Clara USA). 100 bp paired end reads sequencing was performed using the Illumina NovaSeq 6000 system by Edinburgh Genomics (Edinburgh ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Genetics 2020Quote: ... enrichment and elution were performed according to manufacturer’s instructions and protocols (SureSelect Human All Exon Kits Version CRE, Agilent). The library was sequenced on an Illumina HiSEQ 2500 as paired-end 75bp reads ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor mutational burdens were computed using the exonic ranges from the SureSelect Human All Exon v7 panel from Agilent (bed file downloaded from the manufacturer’s website ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: AMACR/p63 immunohistochemistry (IHC) double staining (Monoclonal Rabbit Anti-Human AMACR, clone 13H4, Dako, Code M361601-2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SCA-associated mutations were introduced into human cMyc-mGlu1 using Quikchange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis primers are shown in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Immunology 2024Quote: ... Microarray analysis was performed at TaKaRa-Bio using high-density oligonucleotide array (Human Genome U133 Plus 2.0) and GeneSpring 12.5 software (Agilent Technologies) as described previously (17) ...
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bioRxiv - Genomics 2019Quote: ... The sheared DNA was used to prepare indexed whole exome sequencing libraries using the Agilent SureSelect XT Human all exon v6 + UTR kit (Agilent Technologies ...