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Citations for Agilent :
151 - 200 of 1293 citations for Recombinant Human KDR Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies) and purified using the AdEasy Virus Purification Kit (Agilent Technologies) ...
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bioRxiv - Pathology 2019Quote: ... the sections were incubated with the biotinylated secondary antibody (goat anti-rabbit and goat anti-mouse, Dako) for 60 minutes ...
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bioRxiv - Biophysics 2022Quote: A plasmid encoding 6xHis-tagged methionyl-tRNA synthetase (MetRS) was transformed into BL21 (DE3) Codon+ RIL cells (Agilent). Overnight cultures were diluted (1:100 ...
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bioRxiv - Biophysics 2022Quote: Octahistidine-tagged Sec14 (His8-Sec14) was purified from BL21-CodonPlus (DE3)-RIL cells (Stratagene, La Jolla, CA, USA) as described for Sfh1 (Schaaf et al. ...
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bioRxiv - Neuroscience 2020Quote: The DNA from the patient and his parents was hybridized on a CGH platform (Agilent Technologies). The derivative log ratio spread (DLRS ...
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bioRxiv - Immunology 2022Quote: ... were introduced into pTT3-gH-His using the QuickChange XL II Kit (Agilent, Cat. number #200521) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: High-performance liquid chromatography (HPLC) (Shimadzu Co.) with an ion exchange column (Agilent Hi-Plex Column) was employed to detect the metabolites of first part of pathway and the final product ...
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bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
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bioRxiv - Biochemistry 2020Quote: Human ISG15 cDNA was amplified by RT-PCR from universal human reference RNA (Agilent 740000-41), adding BamHI and NotI restriction sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Cell Biology 2023Quote: Non-codon optimised sequences were amplified from a human cDNA library (MegaMan human transcription library, Agilent). Mis18α ...
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bioRxiv - Genomics 2019Quote: ... SureSelect Human All Exon V6 (Agilent), and the Human Core Exome Kit + RefSeq V1 (Twist) ...
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bioRxiv - Pathology 2019Quote: ... and mouse anti-human CD31 (DAKO) was applied overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Universal Human Reference RNA (UHRR, Agilent) was spiked into each of the samples for a final RNA amount of 300 ng to balance the RNA concentrations in the pools ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human tau (Dako, 1:10,000), the phosphorylation specific anti-tau antibody Ser396/Ser404 (PHF1 ...
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bioRxiv - Neuroscience 2019Quote: ... total tau (total human tau, Agilent), Tau-1 (Millipore) ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... SureSelect Human All Exon V6 (Agilent) exome libraries were prepared and samples were paired-end whole exome sequenced (WES ...
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bioRxiv - Developmental Biology 2021Quote: ... SureSelectXT Human All Exon Kits (Agilent) or Human Core Exome (Twist Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD45 (Dako M0701, 1:100), and human CD20 (Dako M0755 ...
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bioRxiv - Cancer Biology 2023Quote: ... human vimentin (1:4000; M0725, DakoCytomation), breast cancer resistance protein (BCRP ...
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bioRxiv - Immunology 2021Quote: ... MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE; Agilent, Santa Clara, CA) as previously described.46 A decoy reagent to gate out the non-MSP-142 or AMA-1–specific B cells was constructed by conjugating SA-PE to DyLight 650 (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... was used followed by incubation with secondary antibody (biotinylated donkey anti-rabbit) and alkaline phosphatase-labelled streptavidin (Agilent). RED was used as chromogen (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... The LCMV-specific antigen tetramer was prepared by conjugating the biotinylated LCMV-NP to SA-PE (Prozyme PJRS25) at a concentration ratio of 4:1 respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies were detected with a 40-minute incubation with biotinylated universal secondary antibodies (Dako, Carpinteria, CA) and streptavidin–horseradish peroxidase ...
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bioRxiv - Biophysics 2020Quote: ... The recombinant construct was transformed into Arctic express (DE3) competent cells (Agilent Technologies, USA). The transformed cells were grown at 37°C in LB media supplemented with 100 μg/ml ampicillin and 10 μg/ml gentamycin ...
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
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bioRxiv - Biochemistry 2019Quote: ... encoding N-terminally 6xHis tagged MBP-MS2 (Gift from Nadège Liaud, UC-Berkeley) was transformed into BL21 Codon+ RIL chemically competent cells (Agilent). An overnight culture was used to inoculate 3L of ZYM-5052 auto-inducing media (Studier ...
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bioRxiv - Cancer Biology 2020Quote: ... The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent, La Jolla, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The immobilized Hi-C libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with eight PCR amplification cycles ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were separated on a Hi-Plex Na column by high-performance liquid chromatography (HPLC) (Agilent Technologies) by applying a flow rate of 0.7 mL/min with water and methanol as mobile phase ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were analyzed using high-performance liquid chromatography (HPLC) with a Hi-Plex Na column (Agilent Technologies). The separation was achieved by applying a flow rate of 0.7 mL/min ...
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bioRxiv - Developmental Biology 2019Quote: ... Capacity for three-dimensional human blood vessel formation was quantified two weeks later using human CD31 (DAKO) immuno-histochemistry of harvested Matrigel plugs ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... followed by incubation with an anti-human CD31 monoclonal antibody (1:30, mouse anti-human; Dako, Glostrup, 0823) to stain for human endothelium ...
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bioRxiv - Developmental Biology 2020Quote: ... The slides were stained with primary antibody then biotinylated secondary antibodies using a REAL Detection System (LSAB+ kit; Dako). The signals were visualized using a REAL Streptavidin Alkaline Phosphatase kit (Dako) ...
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bioRxiv - Genetics 2022Quote: ... for fragments corresponding to a 10.3-kb genomic region (coordinates 66,234 – 76,516) of the BL21AI genome [14] using custom-designed biotinylated ssDNA probes (Agilent SureSelect) and streptavidin-bead purification ...
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bioRxiv - Neuroscience 2022Quote: ... For PrP deposition anti-PrP monoclonal antibodies ICSM3525,26 were used in conjunction with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Genetics 2020Quote: For pull-down experiments each recombinant version of His6-GST-tagged Sen1 Cter or the His6-GST control was overexpressed by growing BL21 (DE3) CodonPlus (Stratagene) cells harboring the appropriate plasmid (see table S13 ...
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bioRxiv - Biochemistry 2021Quote: Rat Kir6.1 and N-terminal FLAG-tagged (DYKDDDDK) SUR2B were first cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses in HEK293 cells according to manufacturer’s instructions63,64 ...
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bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
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bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
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bioRxiv - Microbiology 2019Quote: ... All GBF1 truncations were introduced into the backbone of Venus tagged-GBF1/A795E (described in 57) using QuickChange XL site-directed mutagenesis kit from Agilent Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: Genes encoding rat Kir6.2Q52R and N-terminal FLAG-tagged (DYKDDDDK) hamster SUR1 were cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...