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Citations for Agilent :
1 - 50 of 776 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... and prediluted polyclonal Guinea Pig anti-human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) as second and third primary antibodies for 1h at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... the CD3 receptor (Agilent technologies ...
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bioRxiv - Physiology 2019Quote: ... Insulin was detected with guinea pig anti-insulin antibody (DAKO, Denmark) followed with secondary Alexa 547 anti-guinea pig (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... anti-insulin (Agilent, USA), and anti-CD3 (Serotec ...
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bioRxiv - Physiology 2021Quote: ... insulin (Dako, Cat# IR002), and glucagon (Abcam ...
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bioRxiv - Immunology 2020Quote: ... INSULIN (DAKO, cat# A0564) and CD45 (DAKO ...
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bioRxiv - Molecular Biology 2024Quote: ... or insulin (IR002; Dako) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-progesterone receptor (DAKO, clone PgR636), anti-HER2 (DAKO ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... Insulin was visualized using an anti-insulin antibody (code A0564, 1:1000 dilution, Dako) and anti-guinea pig Alexa Fluor 594 (1:400 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... Insulin (1:200, DAKO, A0564), Somatostatin (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... guinea pig anti-insulin (Dako). Secondary antibodies (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Insulin (DAKO A0564, 1:200), IRE1α (CST #3294 ...
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bioRxiv - Physiology 2019Quote: ... guinea pig anti Insulin (DAKO), rabbit anti-Calnexin (ENZO) ...
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bioRxiv - Cell Biology 2021Quote: ... insulin (1:500, Dako #A0564) and glucagon (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunofluorescence: insulin (Dako 1:1,000), PDX1 (Abcam AB47383 1:2000) ...
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bioRxiv - Developmental Biology 2023Quote: ... Insulin (1:2, Dako IR002), Integrin-α6 (1:400 ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
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bioRxiv - Physiology 2021Quote: ... anti-insulin (A0564) was from DAKO. The oligonucleotides sequences used for various experiments are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by insulin staining (Dako, A0564). Imaging was performed using an EVOS FL Autofluorescence microscope (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Insulin (1:250; Dako, Catalog# A0564), Insulin (1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... and Guinea Pig anti-insulin (Dako) antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Insulin (Agilent, IR-002, 1:4), and Glucagon (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and stained for insulin (Dako A0564), glucagon (Millipore MABN238) ...
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bioRxiv - Genomics 2019Quote: ... Androgen Receptor (Dako, M3562, 1/200, pH 9). Slides were digitalized using a Hamamatsu NanoZoomer slide scanner (Japan ...
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bioRxiv - Cell Biology 2019Quote: ... guinea pig anti-insulin (DAKO, Houston, TX), rabbit anti-detyrosinated tubulin (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Guinea Pig anti-Insulin (Dako IR00261-2) and mouse anti-TAF4 (TAF II p135 ...
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bioRxiv - Physiology 2022Quote: ... and insulin (Cat. # A0564, Dako, Carpinteria, CA). Each slide contained a negative control section incubated only in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... guinea pig anti-insulin (1:500, Agilent), rat anti- RFP (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Estrogen Receptor alpha (ERα, Clone EP1, Dako IR 084), GATA3 (sc-268 ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used were: Insulin (DAKO, 1:400), C-peptide (Abcam ...
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bioRxiv - Immunology 2021Quote: ... polyclonal guinea pig anti-insulin (undiluted, IR002, Dako) for 20min ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-insulin (1:1000, Dako cat.no A0564). For immunostaining ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... and guinea pig anti-insulin (DAKO; 1:5000) antibodies were used to confirm protein expression in the pancreas.
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bioRxiv - Physiology 2021Quote: ... Plasma insulin was determined with ELISA (Dako Denmark) according to manufacturer’s instructions.