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Citations for Agilent :
51 - 100 of 1454 citations for Recombinant Human ERBB2 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Titer analysis of spent medium was analyzed offline daily by Protein A chromatography on an Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA) as well as with the BLItz biolayer interferometer system (ForteBio ...
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bioRxiv - Immunology 2020Quote: ... streptavidin-PE (ProZyme) was titrated into a fixed amount of biotinylated F at increasing concentrations and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... and PE (Prozyme) (Spike) ...
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bioRxiv - Immunology 2021Quote: ... streptavidin-PE (ProZyme) was added to each biotinylated FcγR in a 4:1 molar ratio and incubated rotating for 10 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... streptavidin-PE (Prozyme) was conjugated to AlexaFluor647 using the Antibody Labeling Kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... FcR binding was quantified by incubating immune complexes with biotinylated FcRs (FcγR2A-1, FcγR2A-2, FcγR3A, courtesy of Duke Protein Production Facility) conjugated to Steptavidin-PE (Prozyme). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Immunology 2023Quote: ... Antigens were tetramerized by incubation at a >4:1 ratio of biotinylated protein with streptavidin-PE (Agilent; PJRS25) or streptavidin-APC (Agilent ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... and CD19 PE (Dako) mAbs for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and phycoerythrin (PE) (ProZyme) and 0.5µg of biotinylated RBD with BV421 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin-PE (Agilent, PJRS25) was added to each biotinylated Fcγ-receptor in a 4:1 molar ratio and incubated rotating for 10min at RT to create the detection reagent ...
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bioRxiv - Immunology 2022Quote: ... streptavidin-PE (PJRS25, ProZyme) was mixed with antigen in increasing ratio and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies tagged with Horse Radish Peroxidase (HRP; Dako) were incubated for 1 h at room temperature under rotation ...
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bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Microbiology 2022Quote: ... streptavidin-PE (ProZyme, cat#PJRS25) was titrated into a fixed amount of biotinylated F at increasing concentrations and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... CD8α-PE (clone: DK25; Dako), CD8β-ECD (clone ...
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bioRxiv - Immunology 2023Quote: ... Streptavidin-PE (Agilent Technologies, Inc.) at 4:1 monomer:streptavidin molar ratio was added in the dark ...
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bioRxiv - Immunology 2024Quote: ... 2,5.105 PMN were incubated with different treatments and then stained for 30 min with a specific mouse anti-human CD11b antibody conjugated with PE (Dako, Santa Clara, CA, USA) at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Labeled RNAs (Liqa Kit, Agilent) were then hybridized onto Agilent whole-mouse genome microarray chips ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP-labeled secondary antibodies (DAKO) followed by incubation with Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Immunology 2022Quote: ... a respective PE–streptavidin (Agilent Technologies) coupled recombinant and biotinylated human FcγR protein was used as a secondary probe ...
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bioRxiv - Bioengineering 2020Quote: ... A streptavidin-phycoerythrin (SA-PE, Prozyme) fluorophore was used to label the biotinylated anti-EGFR aptamer and the antibody ...
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bioRxiv - Immunology 2022Quote: ... PE-streptavidin (Agilent Technologies, CA, USA) at a 1:1000 dilution.
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bioRxiv - Microbiology 2022Quote: ... PE-Streptavidin (Agilent Technologies, CA, USA) was coupled to recombinant and biotinylated mouse FcγR2b ...
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bioRxiv - Immunology 2023Quote: ... and tetramerized with streptavidin-PE (Prozyme) as previously described42 ...
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bioRxiv - Immunology 2023Quote: ... and tetramerized with streptavidin-PE (Agilent) or streptavidin-APC (Agilent ...
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bioRxiv - Immunology 2022Quote: E382R/S/A mutations were introduced into IgG1 Fc encoded within a pFUSE-hIgG1-Fc vector using site-directed mutagenesis (QuikChange II kit, Agilent), using mutagenic primers (Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... Envision labeled polymer (Dako, Glostrup, Denmark) was used for 30 minutes as a secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE; Agilent, Santa Clara, CA) as previously described.46 A decoy reagent to gate out the non-MSP-142 or AMA-1–specific B cells was constructed by conjugating SA-PE to DyLight 650 (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-CD11b-PE (R0841; Dako, Switzerland).
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bioRxiv - Developmental Biology 2019Quote: ... elegans PGL-3 NtDD wild type and mutant recombinant protein were determined by conducting SEC-MALS experiments using Agilent Technologies 1260 LC HPLS system (Agilent Technologies, Santa Clara, CA) equipped with Dawn® Heleos™II 18-angle MALS light scattering detector ...
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bioRxiv - Biochemistry 2019Quote: ... hexahistidine-tagged IN was overexpressed in BL-21 CodonPlus RIL cells (Agilent). Cells were lysed in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
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bioRxiv - Developmental Biology 2020Quote: ... Dual-tagged Slit2 Δ construct was obtained by Quickchange mutagenesis procedure (Agilent), deleting 9 amino acids (SPPMVLPRT ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Immunology 2022Quote: ... the Envision+ System-HRP Labeled Polymer (Dako) was used as a secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... and labeled with Cy3 (CyDye; Agilent Technologies) during in vitro transcription ...