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Citations for Agilent :
51 - 100 of 1411 citations for Recombinant Human CD79B protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Immunology 2020Quote: ... and tetramerized with streptavidin-APC (Agilent) as previously described (Krishnamurty et al. ...
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bioRxiv - Immunology 2020Quote: ... anti-CD8 (clone DK25, APC; Dako), anti-IFN-γ (clone 4S.BS ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and 40 nM streptavidin-conjugated APC (Prozyme) in Lance detection buffer (Perkin Elmer) ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD8 (clone DK25, APC; Dako). Flow cytometry was performed on a FACSCanto II flow cytometer (BD) ...
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bioRxiv - Immunology 2022Quote: ... or streptavidin-APC (0.74mg/mL; Agilent technologies) at an 8:1 molar ratio of monomer:streptavidin ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies tagged with Horse Radish Peroxidase (HRP; Dako) were incubated for 1 h at room temperature under rotation ...
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bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
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bioRxiv - Neuroscience 2022Quote: ... Labeled RNAs (Liqa Kit, Agilent) were then hybridized onto Agilent whole-mouse genome microarray chips ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP-labeled secondary antibodies (DAKO) followed by incubation with Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and PLQPEQPFP was attached to streptavidin-APC (Agilent). After incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Immunology 2023Quote: ... Tetramers were assembled by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Agilent, #PJRS27-1) according to NIH Tetramer Facility protocols ...
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bioRxiv - Immunology 2022Quote: E382R/S/A mutations were introduced into IgG1 Fc encoded within a pFUSE-hIgG1-Fc vector using site-directed mutagenesis (QuikChange II kit, Agilent), using mutagenic primers (Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... Envision labeled polymer (Dako, Glostrup, Denmark) was used for 30 minutes as a secondary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... SIINFEKL-Kb tetramer was prepared using streptavidin-APC (Prozyme) and SIINFEKL-Kb monomer from the NIH Tetramer Core.
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bioRxiv - Immunology 2020Quote: ... The tetramers were made by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Prozyme, Hayward, CA) according to protocols from the NIH tetramer facility ...
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bioRxiv - Developmental Biology 2019Quote: ... elegans PGL-3 NtDD wild type and mutant recombinant protein were determined by conducting SEC-MALS experiments using Agilent Technologies 1260 LC HPLS system (Agilent Technologies, Santa Clara, CA) equipped with Dawn® Heleos™II 18-angle MALS light scattering detector ...
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bioRxiv - Biochemistry 2019Quote: ... hexahistidine-tagged IN was overexpressed in BL-21 CodonPlus RIL cells (Agilent). Cells were lysed in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
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bioRxiv - Developmental Biology 2020Quote: ... Dual-tagged Slit2 Δ construct was obtained by Quickchange mutagenesis procedure (Agilent), deleting 9 amino acids (SPPMVLPRT ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Immunology 2022Quote: ... the Envision+ System-HRP Labeled Polymer (Dako) was used as a secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... and labeled with Cy3 (CyDye; Agilent Technologies) during in vitro transcription ...
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bioRxiv - Biophysics 2024Quote: Accurate masses of unlabeled and labeled proteins were determined via LC-MS with an Agilent PL1912- 1300 reverse phase HPLC column (Agilent, Santa Clara, CA, USA) connected to an AB Sciex 5600 QTOF mass spectrometer (AB Sciex ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies, United States) according to the manufacturer’s instructions and the respective parental vector as template ...
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bioRxiv - Biochemistry 2020Quote: ... Selenomethionine (SeMet)-labeled and native proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RP-X and BL21-CodonPlus (DE3)-RIL (Agilent Technologies, Santa Clara, CA, USA), respectively ...
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bioRxiv - Immunology 2022Quote: ... 1µg of biotinylated spike with either streptavidin-conjugated allophycocyanin (APC) (ProZyme) and phycoerythrin (PE ...
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bioRxiv - Immunology 2023Quote: ... biotinylated TG2 and TG3 were attached to APC-conjugated streptavidin (Agilent) and PE-conjugated streptavidin (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Phycoerythrin (PE)- or allophycocyanin (APC)-conjugated streptavidin were obtained from Agilent. The QFL dextramers were generated following the ‘dextran doping’ technique(Bethune et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... After performing Fc block by using blocking buffer (Dako, X0909), cells were stained by CD24 (Abcam ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Cell Biology 2021Quote: ... followed by Envision + System-HRP labeled Polymer (Dako). Immunolabeling was visualized with Liquid DAB + Substrate (Dako ...
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bioRxiv - Physiology 2020Quote: ... Positive cells were labeled by DAB chromogen (DAKO) for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeled DNA was diluted in hybridization buffer (Agilent) and hybridized to arrays for 24 h at 65°C ...
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bioRxiv - Immunology 2022Quote: ... labeled iRBCs were mixed with 30ug PE (Agilent) prior to injection.
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bioRxiv - Microbiology 2023Quote: ... then labeled using Cyanin-3 CTP (Agilent Technologies) and hybridized on chips containing 45000 probes (4×44K Whole Human Genome) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by rabbit-labeled polymer HRP (Agilent, K4011) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminally His6-tagged KRAS was expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl-β-D-1-thiogalactopyranoside induction at 18 °C ...
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bioRxiv - Cell Biology 2022Quote: ... HA-tagged DCX mutant T203R were created using QuikChange Site-Directed Mutagenesis kit (Stratagene). HA-tagged DCX mutant A71S was synthesized commercially (Genewiz ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...