Labshake search
Citations for Agilent :
151 - 200 of 3042 citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... pShuttleCMV-Bub1K795R was used to generate recombinant adenoviruses using the AdEasy system (Stratagene), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies) ...
-
bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
-
bioRxiv - Biophysics 2023Quote: ... Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies) and purified using the AdEasy Virus Purification Kit (Agilent Technologies) ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
-
bioRxiv - Neuroscience 2020Quote: The DNA from the patient and his parents was hybridized on a CGH platform (Agilent Technologies). The derivative log ratio spread (DLRS ...
-
bioRxiv - Immunology 2022Quote: ... were introduced into pTT3-gH-His using the QuickChange XL II Kit (Agilent, Cat. number #200521) according to manufacturer’s instructions.
-
bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
-
bioRxiv - Bioengineering 2023Quote: High-performance liquid chromatography (HPLC) (Shimadzu Co.) with an ion exchange column (Agilent Hi-Plex Column) was employed to detect the metabolites of first part of pathway and the final product ...
-
bioRxiv - Biophysics 2022Quote: A plasmid encoding 6xHis-tagged methionyl-tRNA synthetase (MetRS) was transformed into BL21 (DE3) Codon+ RIL cells (Agilent). Overnight cultures were diluted (1:100 ...
-
bioRxiv - Biophysics 2022Quote: Octahistidine-tagged Sec14 (His8-Sec14) was purified from BL21-CodonPlus (DE3)-RIL cells (Stratagene, La Jolla, CA, USA) as described for Sfh1 (Schaaf et al. ...
-
bioRxiv - Biochemistry 2020Quote: Human ISG15 cDNA was amplified by RT-PCR from universal human reference RNA (Agilent 740000-41), adding BamHI and NotI restriction sites at the 5’ and 3’ ends respectively ...
-
bioRxiv - Cell Biology 2023Quote: Non-codon optimised sequences were amplified from a human cDNA library (MegaMan human transcription library, Agilent). Mis18α ...
-
bioRxiv - Developmental Biology 2019Quote: ... All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako, M0823, 1:100) and Alexa488-conjugated goat anti-mouse secondary antibody (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
-
bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
-
bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
-
bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
-
bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
-
bioRxiv - Genomics 2019Quote: ... SureSelect Human All Exon V6 (Agilent), and the Human Core Exome Kit + RefSeq V1 (Twist) ...
-
bioRxiv - Pathology 2019Quote: ... and mouse anti-human CD31 (DAKO) was applied overnight at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... Universal Human Reference RNA (UHRR, Agilent) was spiked into each of the samples for a final RNA amount of 300 ng to balance the RNA concentrations in the pools ...
-
bioRxiv - Neuroscience 2019Quote: ... total tau (total human tau, Agilent), Tau-1 (Millipore) ...
-
The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... SureSelect Human All Exon V6 (Agilent) exome libraries were prepared and samples were paired-end whole exome sequenced (WES ...
-
bioRxiv - Developmental Biology 2021Quote: ... SureSelectXT Human All Exon Kits (Agilent) or Human Core Exome (Twist Bioscience ...
-
bioRxiv - Neuroscience 2022Quote: ... and CD68 (Monoclonal Mouse Anti-Human, EDTA Buffer HIER, IHC dilution 1:5000, IF dilution 1:500, Clone L26, Dako Omnis, Code Number: M0756) were employed to characterize lympho-monocytic infiltrations ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 µg of tumor DNA extracted from fresh-frozen tissue and 1 µg of male human reference DNA control (Agilent Technologies, Santa Clara, CA) were digested with AluI and RsaI ...
-
bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
-
bioRxiv - Biophysics 2020Quote: ... The recombinant construct was transformed into Arctic express (DE3) competent cells (Agilent Technologies, USA). The transformed cells were grown at 37°C in LB media supplemented with 100 μg/ml ampicillin and 10 μg/ml gentamycin ...
-
bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
-
bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
-
bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
-
bioRxiv - Biochemistry 2019Quote: ... encoding N-terminally 6xHis tagged MBP-MS2 (Gift from Nadège Liaud, UC-Berkeley) was transformed into BL21 Codon+ RIL chemically competent cells (Agilent). An overnight culture was used to inoculate 3L of ZYM-5052 auto-inducing media (Studier ...
-
bioRxiv - Cancer Biology 2020Quote: ... The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent, La Jolla, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The immobilized Hi-C libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with eight PCR amplification cycles ...
-
bioRxiv - Microbiology 2023Quote: Pharmaceuticals were separated on a Hi-Plex Na column by high-performance liquid chromatography (HPLC) (Agilent Technologies) by applying a flow rate of 0.7 mL/min with water and methanol as mobile phase ...
-
bioRxiv - Microbiology 2023Quote: Pharmaceuticals were analyzed using high-performance liquid chromatography (HPLC) with a Hi-Plex Na column (Agilent Technologies). The separation was achieved by applying a flow rate of 0.7 mL/min ...
-
bioRxiv - Developmental Biology 2019Quote: ... Capacity for three-dimensional human blood vessel formation was quantified two weeks later using human CD31 (DAKO) immuno-histochemistry of harvested Matrigel plugs ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
-
bioRxiv - Genetics 2020Quote: For pull-down experiments each recombinant version of His6-GST-tagged Sen1 Cter or the His6-GST control was overexpressed by growing BL21 (DE3) CodonPlus (Stratagene) cells harboring the appropriate plasmid (see table S13 ...
-
bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
-
bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
-
bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
-
bioRxiv - Microbiology 2019Quote: ... All GBF1 truncations were introduced into the backbone of Venus tagged-GBF1/A795E (described in 57) using QuickChange XL site-directed mutagenesis kit from Agilent Technology ...
-
bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
-
bioRxiv - Biochemistry 2024Quote: Genes encoding rat Kir6.2Q52R and N-terminal FLAG-tagged (DYKDDDDK) hamster SUR1 were cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses ...
-
bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
-
bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
-
bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...