Labshake search
Citations for Agilent :
451 - 500 of 1199 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... the tumor and tumoroid sections were mounted on the slide with Dako Fluorescent Mounting Medium (Agilent, Santa Clara CA, USA). Samples without the addition of primary antibody were used as negative control ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... OCI-AML3 and NALM6 cells stained with PBS containing 0.1 μg/mL acridine orange were evaluated for GFP and RFP channels using a fluorescent microscope (Lionheart FX Automated microscope; Agilent BioTek Instruments Inc. ...
-
bioRxiv - Cell Biology 2023Quote: ... then washed 3 × 10minute and post-fixed with 0.1% PFA for 10minutes and mounted using fluorescent mounting medium (DAKO, USA).
-
bioRxiv - Microbiology 2023Quote: ... and the fluorescent emission (excitation at 486-580 nm and emission at 568-590 nm) was measured using Synergy HT (BioTek, Agilent). Uncoated beads and beads coated with BSA were used as controls ...
-
bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
-
bioRxiv - Cell Biology 2023Quote: ... All sections were washed three times for 3 min with PBS and mounted with Dako Fluorescent Mounting Medium (Agilent, USA).
-
bioRxiv - Neuroscience 2024Quote: ... sections were washed with PBS for 15 mins (3×5min fresh solution) and cover-slipped with fluorescent mounting medium (Dako).
-
bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hr at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope ...
-
bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR analyses were performed using Brilliant III Ultra-Fast SYBR® Green (Agilent) after DNase treatment using Turbo DNase (AMBION) ...
-
bioRxiv - Genetics 2020Quote: ... qPCR was performed with Brilliant II SYBR® Green QPCR Master Mix (Agilent Technologies) in a LightCycler® 480 Instrument II (Roche) ...
-
bioRxiv - Genetics 2020Quote: qPCR was performed with Brilliant II SYBR® Green qPCR Master Mix (Agilent Technologies) in a LightCycler® 480 Instrument II (Roche) ...
-
bioRxiv - Neuroscience 2020Quote: ... Brilliant-III ultrafast SYBR Green qPCR master mix (Agilent Technologies, Santa Clara, CA, USA) and ProteoStat-aggresome detection kit (Enzo Life sciences ...
-
bioRxiv - Cell Biology 2021Quote: ... qPCRs were realized using SYBR® Green Real-Time PCR Master Mix kit (Agilent Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... the Brilliant III Ultra-Fast SYBR® Green one-step qRT-PCR kit (Agilent) was used ...
-
bioRxiv - Physiology 2020Quote: ... q-RT-PCR was performed using Brilliant III SYBR Green QPCR Master Mix (Agilent) on the StepOne+ Real-Time PCR system (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2019Quote: ... and 7.5 µl of Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... 12.5 μL of 2x Brilliant II SYBR Green Master Mix with low ROX (Agilent), and 9.3 μL of nuclease-free water ...
-
bioRxiv - Immunology 2021Quote: ... and a Brilliant III SYBR Green Master mix (Agilent Technologies, Santa Clara, CA, USA) with specific primer pairs (Table 2) ...
-
bioRxiv - Microbiology 2022Quote: ... The Brilliant III Ultra-Fast SYBR® Green one-step qRT-PCR kit (Agilent) was used for all qRT-PCR reactions ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies, USA) on the Roche LightCycler480 PCR system (1 min 95 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... using 10 uL of Brilliant III Ultra-Fast SYBR Green Master-Mix (Stratagene, USA), 1 μL of cDNA ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein Block Serum Free reagent (Dako). Primary antibody incubation was performed at 4°C overnight using the ideal dilution for each antibody (Supplementary Table 5) ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated in Protein Block (Dako) for 5 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each ...
-
bioRxiv - Microbiology 2023Quote: ... AdvanceBio SEC 300Å protein standard (Agilent) was included to correlate the elution volume with molecular weight.
-
bioRxiv - Microbiology 2023Quote: ... diluted with protein block buffer (Dako) for 2 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
-
bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
-
bioRxiv - Immunology 2020Quote: ... The recombinant Ad5 genomes with HA inserts were linearized with PacI and buffer exchanged using a Strataprep PCR purification kit (Agilent Technologies). The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen) ...
-
bioRxiv - Biophysics 2019Quote: Human myosin IIa S1 and lever-arm-less S1: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies) ...
-
bioRxiv - Neuroscience 2019Quote: ... After one last wash with DPBS for 5 minutes at RT the coverslips were imbedded in fluorescent mounting medium (DAKO #S3023). Primary antibodies that were used ...
-
bioRxiv - Genomics 2021Quote: ... Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Libraries were sequenced on an Illumina NovaSeq 6000 system using 2 x 150bp cycles.
-
bioRxiv - Neuroscience 2021Quote: ... the slides were counterstained with DAPI for 30 sec at RT and mounted using Dako fluorescent mounting medium (Agilent Technologies, USA). The dyes used for signal detection were Opal Dye 520 and Opal Dye 570 (Akoya Biosciences ...
-
bioRxiv - Biochemistry 2022Quote: ... The fluorescent labeled oligosaccharides were separated and quantified by electrophoresis using an OLIGO Profiling Gel and a FACE imaging system (ProZyme, Inc.). The stoichiometry of labeling was such that only one molecule of fluorophore was attached to each molecule of oligosaccharide ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were washed four times with PBS containing Ca2+ and Mg2+ and mounted in a fluorescent mounting medium (DAKO, CA, USA). Finally ...
-
bioRxiv - Cell Biology 2021Quote: ... washed three times in PBST containing 10 µg/ml DAPI for 5 minutes and mounted with a coverslip in fluorescent mounting media (Dako, S3023). All images were taken using a Zeiss LSM 710 confocal microscope and quantified manually using ImageJ software ...
-
bioRxiv - Cell Biology 2021Quote: ... Fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100) were used to evaluate final libraries ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections or cell containing coverslips were mounted on gelatin coated slides with Fluorescent Mounting Medium (S3023, Dako Cytomation, Glostrup, Denmark). The following primary antibodies were used ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR reactions using SYBRgreen fluorescent reagent and/or EvaGreen™ were achieved on the Stratagene Mx3000P system (Agilent Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were mounted in glass slides using 0.2% gelatine in TB and cover-slipped with fluorescent mounting medium (FluorSave Tm Reagent; Dako, Glostrup, Denmark).
-
bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 5 x 15 min in PBST at RT and slides were mounted in DAKO fluorescent mounting medium (Agilent Technologies). EdU staining was performed on sections from larvae that received an EdU pulse ...
-
bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
-
bioRxiv - Molecular Biology 2019Quote: ... Second-round PCR was performed with SYBR Green and real-time PCR conditions (Mx3005p, Agilent) (Brunet et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using SYBR Green One-Step (Kit# 600825, Agilent, Santa Clara, CA). qRT-PCR data were analyzed using the Sequence Detection System software (SDS Version 2.2 ...
-
bioRxiv - Plant Biology 2020Quote: ... or Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent, Santa Clara, CA, USA) on either an ABI 7500 or Agilent AriaMX qPCR system ...