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Citations for Agilent :
451 - 500 of 500 citations for Rat TMEM165 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: 375W substitutions in all the primary isolates were introduced by site-directed mutagenesis in plasmids carrying Envs using a QuikChange site-directed mutagenesis kit (Stratagene Inc.) and mutagenic primers to introduce each mutation (56) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutations G to C at positions 445 and 457 were introduced into DCP2 in plasmid pQZ145 (Zeidan et al. 2018) using primers AKV005/AKV006 and the Quick-change Site-Directed mutagenesis kit (Agilent, 200519), generating plasmid pAV008 containing dcp2-E149Q,E153Q ...
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bioRxiv - Molecular Biology 2023Quote: ... The T94D mutation was introduced in the plasmids by PCR using the QuickChange Multi-Site Directed Mutagenesis kit (#200514 -Agilent Technologies). The sequence of the primer used to introduce the mutation T94D in the Nme1 sequence of plasmids is reported in key resources table ...
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bioRxiv - Molecular Biology 2023Quote: ... The mutagenesis of the HDE-like motifs and PAM sequence within donor plasmid for HDR was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). pcDNA3.1(+)-ARRDC4-1 and pcDNA3.1(+)-ADCYAP1-2 expression vectors were constructed by cloning lnc-ARRDC4-1 and lnc-ADCYAP1-2 sequences to pcDNA3.1(+ ...
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bioRxiv - Genetics 2022Quote: ... Three Myc epitopes were then inserted before the stop codon into the three plasmids with the QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies) to get SLITRK3-WT-Myc ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Substitutions D479N and D499N were introduced into the PRORP expression plasmid by site-directed mutagenesis using the QuikChange protocol (Agilent Technologies). The two variants were expressed and purified like the wild type protein (19).
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bioRxiv - Microbiology 2023Quote: ... into AAATA in the DENV-2 wild type plasmid (pFK-DVs) was generated using QuickChange XL site-directed mutagenesis kit (Agilent, 200517) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Biochemistry 2023Quote: Mutations were generated by site-directed mutagenesis (table 2) on this plasmid using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and plasmids were verified through sequencing through the Molecular Informatics Core at the University of Rhode Island.
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bioRxiv - Molecular Biology 2021Quote: ... The T695A and T656A mutations were generated in WT-YME1L1 plasmid via QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were co-transfected with 100ng pCAG-Lgr6 overexpression plasmid (pLgr6) or pBluescript II KS+ (pBKS) (Agilent Technologies, Santa Clara, CA, USA) as a control ...
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bioRxiv - Molecular Biology 2020Quote: ... EcoRV and SacI restriction sites that flanked the B3 domain were introduced into the VP1-TOPO plasmid by site-directed mutagenesis (Agilent Technologies, CA); 2 ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Lens was introduced to the leader region of the MC-II through MC-VI and MC-V-dotO arrays in our donor plasmids by quickchange PCR with Pfu Turbo Polymerase (Agilent #600250-52). boxA(−58 ...
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bioRxiv - Neuroscience 2022Quote: Co-transfection of pAAV-CAG-DIO-(hCAR)off-(GtACR2Kv2.1-MuGFP)on-W3SL with pDPI and pDPII plasmids (Grimm et al., 2003) into AAV293 cells (Agilent Technologies, CA, USA) preceded harvesting and iodixanol gradient purification (as described by (Zolotukhin et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutants of KflaHY2 were generated in the pET28a_KflaHY2 plasmid using the QuikChange® Lightning kit (Agilent, Santa Clara, California, USA) employing primers listed in Table S1 ...
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bioRxiv - Genetics 2023Quote: ... 15 PCR-2 reactions were completed using Oligo 45 paired with Oligos 47-61 to amplify from YCp50-WT_PKR plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kilo-base pairs ...
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bioRxiv - Cell Biology 2023Quote: pLV-CMV-IRIS-PURO-c-Src-mScarlet plasmid was used to the generation of Y527F and Y527F/K295R mutants via QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200523) according to manufacturer’s protocol using the primers listed in Table 1.
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bioRxiv - Neuroscience 2023Quote: ... the four 130 bp α-unit DNA fragments were amplified from each α-unit plasmid using the Herculase II Fusion DNA polymerase (Agilent) and oJS2581 and oJS2582 primers77 ...
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bioRxiv - Cell Biology 2023Quote: ... GYF motif mutant GIGYF1 (GIGYF1 GYF Mut; G502A, Y503A, F504A) plasmids were created using the QuikChange II Site-Directed mutagenesis kit (Agilent Technologies, 200523). The chimeric constructs were synthesized by GenScript (Piscataway ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Cell Biology 2020Quote: pSecTagNC + ΔEGF_mN1 W1758A and pSecTagNC + ΔEGF_mN1 R1994A: these plasmids were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pSecTagNC + ΔEGF_mN1 as a template and primers ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD R1994A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primer A2026VmutF.
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bioRxiv - Physiology 2020Quote: ... The QuikChange II Site-Directed Mutagenesis protocol was performed using 200 ng of plasmid DNA and PfuTurbo DNA Polymerase according to the manufacturer’s protocol (Agilent Technologies, Santa Clara, CA). Mutagenesis reactions were then digested with Dpn I (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... and Nup133-mEGFP(-7) were cloned from the repair template plasmids constructed above via the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). For each CRISPR cell line ...
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bioRxiv - Biophysics 2021Quote: ... were created using a 1.3mer HBV plasmid (gifted by Dr. Haitao Guo)(59) and site-directed mutagenesis kit (QuikChangeII, Agilent Technologies, Santa Clara, CA) with corresponding primers (University of Calgary DNA Synthesis Lab ...
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Neuroscience 2020Quote: Adeno-associated viruses were made in HEK293T cells by co-transfecting each of the above plasmids with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) (Petrs-Silva et al. 2011) and pHelper (Stratagene, La Jolla, California) plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... The CPE element was identified bioinformatically and was deleted from the plasmid using site-directed mutagenesis (Lightning QuikChange Mutagenesis, Agilent, Santa Clara, CA). DNA sequencing of these plasmids indicated successful deletion (Genewiz ...
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bioRxiv - Cell Biology 2022Quote: ... were introduced into the plasmid pED-FLAG-LMAN1 and pcDNA-MCFD2-Myc separately using the QuickChange site-directed mutagenesis II XL kit from Agilent (Santa Clara, CA). FVIII mutant constructs Δ807–816 (deletion of amino acids 807-816 of FVIII ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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bioRxiv - Biochemistry 2024Quote: ... and L325A—were created using plasmids harboring the wild type GNNV-P sequence and a QuickChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA). Mutations were confirmed by PCR sequencing (Genomics Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The R682S furin cleavage mutation was introduced into the SARS-CoV-2 S expression plasmid by QuikChange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK-293T cells were co-transfected with the following plasmids: pAAV/L7-6-Slc9A6-GFP or pAAV/L7-6-GFP (control) with pHelper (Stratagene, La Jolla, CA, USA), and pUCmini-iCAP-PHP.eB followed by viral particles purification ...
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bioRxiv - Cancer Biology 2019Quote: ... we modified the PTK-ATF4-Luc plasmid from (Dey et al., 2010)39 using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent; Santa Clara, California, USA) to introduce two BsmBI restriction sites flanking the ATF4 5’ UTR ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Microbiology 2019Quote: ... The constitutive firefly luciferase expression plasmid pKM19 was generated by amplifying the firefly luciferase gene from pLUC-MCS (Agilent Technologies, Santa Clara, CA USA) and cloning it into pIEx-EGFP after the enhanced GFP (EGFP ...
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bioRxiv - Microbiology 2020Quote: ... and HIS6-ASH4S124A (rASH4S124A) were expressed from the pIF22 and pIF22-S124A plasmids in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245). Expression was induced with 0.5 mM IPTG (isopropyl-B-D-thiogalactopyranoside ...
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bioRxiv - Immunology 2021Quote: QuikChange Lightening Site-Directed Mutagenesis kit was used to generate amino acid substitutions in the SARS-CoV-2 Wuhan Spike expression vector (Seow et al., 2020) or the D614G pCDNA Spike plasmid (S. A. Kemp et al., 2020) following the manufacturer’s instructions (Agilent Technologies, Inc., Santa Clara, CA). Spike B.1.1.7 was synthesised by Genewiz ...
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bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the resulting megaprimer was inserted at the D93 site (replacing D93 residue) in the PolI-S Can plasmid by QuikChange mutagenesis (Agilent Technologies, Santa Clara, CA). NP-DYFP then was adapted and extracted from the modified PolI-S Can plasmid by PCR (forward primer ...
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bioRxiv - Evolutionary Biology 2024Quote: VPg mutations D113G and R118H were introduced by site-directed mutagenesis in the p35STunos plasmid using the Quikchange II XL kit (Agilent Technologies, Santa Clara CA, USA) and following the indications of the manufacturer ...