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Citations for Agilent :
201 - 250 of 8310 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... followed by another containing 3 μM BPTES (all XFp Mito Fuel Flex Test Kit, Agilent Technologies, 103270-100) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... containing an AgeI restriction site in the FLAG sequence created by site-directed mutagenesis (QuikChange Mutagenesis kit, Agilent) using oligonucleotides detailed in Reagents and Tools table.
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-Glial fibrillary acidic protein (GFAP; 1:1000, Dako, Cat# Z033429, RRID:AB_10013382), mouse anti-CD68 (ED1 ...
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bioRxiv - Neuroscience 2024Quote: ... Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako, Denmark, Z0334, dilution 1:200), Rabbit Anti-Iba1(Fujifilm ...
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bioRxiv - Microbiology 2024Quote: ... rabbit glial fibrillary acidic protein antibody (anti-GFAP,1:500; Dako, Glostrup, Denmark), goat ionized calcium binding adaptor molecule 1 antibody (anti-Iba1,1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were re-suspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cell Biology 2022Quote: ... organoids were incubated in blocking buffer containing primary antibody (rabbit anti-lysozyme 1:800, Dako #A0099) overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Epidemiology 2019Quote: ... OGTT plasma insulin concentrations were measured by ELISA (Dako UK Ltd., Ely, Cambs, U.K.). Intra-assay imprecision (CV ...
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bioRxiv - Cell Biology 2020Quote: ... Cholesterol and its influence on Gb3 binding were analyzed by targeted LC-QqQ-MS (6460, Agilent Technologies) operated in SRM/MRM mode ...
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bioRxiv - Microbiology 2021Quote: ... Binding of the primary antibody was detected using Envision rabbit polymer with AEC as the chromogen (Dako). Each tissue section was evaluated independently by two pathologists ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Physiology 2021Quote: ... Anti-mouse and anti-rat IgG-FITC antibodies were from Dako and the GAPDH antibody from Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and the slides were blocked for 1 h with Serum Free Protein Block (Dako). Slides were incubated with antibodies directed against PARP-1 (Abcam ab194586) ...
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bioRxiv - Molecular Biology 2021Quote: ... After blocking for 1 hour in serum-free protein blocking buffer (DAKO, catalog X0909), sections were incubated overnight at 4°C with anti–phospho-hsp25 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... proteins in the supernatant were precipitated with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 10mM glucose (103577, Agilent), 2mM Glutamine (103579 ...
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bioRxiv - Biophysics 2020Quote: His-tagged CrSAS-6[NL] spanning amino acids 1-503 of the protein (see Supplementary Fig. 1a)1 was expressed in the Escherichia coli strain BL21(DE3) (Stratagene). Bacteria were grown at 37 °C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2020Quote: ... All E protein variants were obtained by site-directed mutagenesis using QuikChange kit (Stratagene, La Jolla, California) and were confirmed by sequencing the plasmid DNA at Macrogen Company (Seoul ...
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bioRxiv - Microbiology 2022Quote: ... The MHC-II binding site mutations were introduced into pKK33 by site-directed mutagenesis (QuickChange II, Agilent Technologies) using the primer set SECF44A/L45Afor/ SECF44A/L45Arev ...
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bioRxiv - Microbiology 2020Quote: ... MinD and ClpX mutant proteins were constructed by site-directed mutagenesis of plasmids containing minD or clpX using the QuickChange II XL Site-directed mutagenesis kit (Agilent) and confirmed by sequencing prior to purification ...
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Genetics 2020Quote: ... plasmid using oligonucleotides containing the SHRF syndrome-linked G58V and G226D missense mutations and the QuikChange Site-Directed Mutagenesis Kit (Stratagene). The untagged RRP4/rrp4-G226D (pAC3656 ...
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bioRxiv - Molecular Biology 2021Quote: ... psub-201 [61] containing the full-length AAV2 genome as template and the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... α-FLAG MAb (rat) (M2) was purchased from Agilent (Santa Clara, CA). α-HA MAb (mouse) ...
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bioRxiv - Immunology 2024Quote: ... for 1.5h at RT and HRP-conjugated rabbit anti-rat (Agilent, P0450) for 1h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
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bioRxiv - Systems Biology 2024Quote: ... VA) or glial fibrillar acidic protein (GFAP; 1:1000; G3893-100uL; Dako, Santa Clara, CA). For Iba1 staining ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Immunology 2019Quote: ... AGP-1 containing fractions were pooled and concentrated by spin concentrators (10 kDa cut off) (Agilent Technologies, USA). The concentrate was then applied to a DEAE-cellulose column (25 x 0.5 cm ...
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bioRxiv - Genetics 2020Quote: ... Captured libraries were amplified in 3×100 μl reactions containing 1 unit Herculase II Fusion DNA polymerase (Agilent), 1x Herculase II reaction buffer ...
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bioRxiv - Biochemistry 2020Quote: ... AGP-1 containing fractions were pooled and concentrated by spin concentrators (10 kDa cut off) (Agilent Technologies, USA). The concentrate was then applied to a DEAE-cellulose column (25 × 0.5 cm ...
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid pEYFP-NONO containing siRNA resistance site corresponding to siRNA NONO s9612 was made by QuikChange site-directed mutagenesis kit (Stratagene, 200518) with base-pair substitutions and the template plasmid pEYFP-C1-NONO (human) ...
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bioRxiv - Biochemistry 2019Quote: ... hhp1 and hhp2 mutants were created by mutagenizing pIRT2 plasmids containing hhp1+ and hhp2+ using a QuikChange site-directed mutagenesis kit (Agilent Technologies). For protein production ...
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bioRxiv - Cell Biology 2019Quote: ... hFN10 containing a TAG stop codon at position 1493 was generated by PCR-based mutagenesis with the Quick-change kit (Agilent Technologies), cloned into bacterial expression plasmid pET11a and verified by DNA sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding the ACR-16 TM3-4 loop or the SDN-1 intracellular domain full length or lacking the EYFA PDZ binding motif were cloned to pGEX-3X expression vector and then transfected into Arctic express E.coli strain (Agilent). Bacterial clones were first cultured overnight in 5 mL LB medium supplemented with 100 mg/mL ampicillin ...
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bioRxiv - Developmental Biology 2023Quote: ... Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies; CatNo. 200521-5). Primers used are shown in Additional file 1 ...
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bioRxiv - Pathology 2023Quote: ... Specific antibody binding was detected using a second antibody and the LSAB system (Dako Corporation, Carpinteria CA, USA, K690) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: Sensor-containing Seahorse fluxpaks (Agilent Technology) were incubated overnight at 37°C in a non-CO2 humidified incubator ...
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bioRxiv - Developmental Biology 2022Quote: ... Sensor-containing Seahorse XFe96 cartridges (Agilent) were hydrated with sterile ultrapure water overnight at 37°C as per manufacturer’s instructions ...