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Citations for Agilent :
201 - 250 of 7722 citations for Rat Phosphoprotein associated with glycosphingolipid enriched microdomains 1 PAG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... the HRP-Anti-Rat IgG (MP-7444, Vector) for 45 min or the Goat Anti-Mouse Immunoglobulins/HRP (Dako-Agilent, P0447) at 1:100 for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 μg total RNA template per reaction was used using High Capacity cDNA Reverse Transcription Kit (Agilent). RNAase-free water (total 4.2 μl) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and their integrity was assessed via 1% agarose gel electrophoresis or Bioanalyzer RNA 6000 Nano kit (Agilent). Library quantification ...
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bioRxiv - Cancer Biology 2023Quote: ... or a High Sensitivity NGS Fragment Analysis Kit (1 bp - 6,000 bp) on a Fragment Analyzer (Agilent). Libraries were quantified by Qubit dsDNA HS Assay (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: ... 1 μg RNA was used to synthesise cDNA using the Multi-temp cDNA Synthesis kit (Agilent, #200436). The final reaction volume was made up to 1ml with nuclease free water and used for RT-qPCR analysis.
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bioRxiv - Physiology 2022Quote: ... Immunologic), the HRP-Anti-Rat IgG (MP-7444, Vector) for 45 min or the Goat Anti-Mouse Immunoglobulins/HRP (Dako-Agilent, P0447) at 1:100 for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-EGFP was made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... anti-SOX17, and anti-SOX9 (MerckMillipore, AB5535, 1:300) antibodies and visualization with EnVision FLEX Mini kit (DAKO).
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bioRxiv - Biophysics 2022Quote: ... HIV-1 GagG2A -EGFP was made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). Venus6 was a gift from Steven Vogel (76 ...
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bioRxiv - Microbiology 2021Quote: ... and DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp −6000 bp; Agilent, Santa Clara, CA, USA). Average library length was 341 bp ...
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bioRxiv - Genetics 2020Quote: ... 1 μL of 4sU-labelled RNA was quality-checked using the Agilent RNA 6000 Pico kit (Agilent technologies) according to the manufacturer’s instructions and run on a 2100 Bioanalyzer Instrument (Agilent).
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bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
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bioRxiv - Plant Biology 2022Quote: ... and the Agilent High Sensitivity NGS Fragment Kit (1-6000 bp) (Cat. No. DNF-474-500; Agilent, USA). The prepared library was pooled for paired-end sequencing using Illumina the Novaseq 6000 at Taiwan Genome Industry Alliance Inc ...
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bioRxiv - Microbiology 2023Quote: ... BQ.1.1 and XBB.1 was constructed by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) using the BA.2 or BA.4/5 SΔ19 gene as a template ...
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bioRxiv - Cell Biology 2023Quote: ... Library quality was assessed on a fragment analyser instrument using SS NGS fragment kit (1-6,000 bp; Agilent). Sequencing was performed on NovaSeq 6000 platform using a 200 cycles SP Reagent Kit v1.5 (both Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Site mutagenesis was performed on pCMV4 CYP2D6*1 plasmid23 with QuikChange II Site-Directed Mutagenesis Kit (Agilent, CA, US). Plasmid cDNA encoding variants with the following amino acid exchanges were created ...
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The Telomere PNA Kit/FITC kit from Dako (Agilent) was used to estimate Telomere length signal in samples ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... An AgeI site was inserted into exon 1 of Cacna1b by mutagenesis using a QuickChange II XL site-directed mutagenesis kit (Stratagene) with primers 1-For and 1-Rev ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... the relevant tetrapeptide motifs were inserted into iBox-PAK4cat within the pXJ40 vector [1] using the Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The prepared library was quantified using Qubit and validated for quality by running an aliquot (1 μl) on High Sensitivity DNA Kit (Agilent) which showed expected fragment distribution in the range of ~250–500 bp ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cy3-labelled probes were prepared from RNA using Low Input Quick Amp Labeling Kit 1-color (Agilent Technologies, #5190-2305) and hybridized with the custom microarrays (ID:085740 ...
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bioRxiv - Genomics 2021Quote: ... The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent). Enriched libraries were loaded with a final concentration of 10 pM on a MiSeq Flow Cell using v3 reagent chemistry for 2×150 cycles.
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...