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Citations for Agilent :
201 - 250 of 5544 citations for Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... rabbit anti-VWF (A0082, 1:1000) and rabbit anti-VWF-HRP (P0226, 1:8000) used for sandwich ELISA were purchased from Dako, sheep anti-VWF (ab11713 ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed between all experimental step with PBS plus 0.1% Tween 20 (405 TS ELISA Plate Washer, Agilent Technologies). Blocking (1% Omniblok ...
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bioRxiv - Immunology 2023Quote: Antibodies against SARS-CoV-2 were measured using a high-throughput direct chemiluminescent ELISA performed on MicroLab STAR robotic liquid handlers (Hamilton) fitted with a 405TS/LS LHC2 plate washer (Biotek/Agilent) (full methods described previously)27 ...
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bioRxiv - Microbiology 2021Quote: Organic acids and ethanol were measured by high-performance liquid chromatography (Agilent, 1260 Infinity), using a standard analytical system (Shimadzu ...
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bioRxiv - Bioengineering 2020Quote: ... butyric acid and glucose were measured using a high-performance liquid chromatography (HPLC, Agilent Technologies 1260 Infinity series ...
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bioRxiv - Biochemistry 2019Quote: ... Amino acid substitutions were performed either with QuikChange XL Site-Directed Mutagenesis (Agilent Technologies) or with a megaprimer site-directed mutagenesis protocol (Kirsch and Joly ...
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bioRxiv - Plant Biology 2019Quote: ... The fatty acid compositions were determined using an Agilent 6890 gas chromatograph (Agilent Technologies) equipped with a DB-23 column (30 m × 0.25 mm × 0.25 μm ...
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bioRxiv - Biophysics 2022Quote: ... Nucleic acid purification was performed using a high-performance liquid chromatography (HPLC, Agilent 1100) equipped with a diode array detector ...
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bioRxiv - Synthetic Biology 2023Quote: ... Residual sugars and organic acids were measured using a HPLC 1100 (Agilent Technologies, USA) equipped with an Aminex 75H column with 4 mM sulfuric acid as the mobile phase at 0.6 mL/min and analyzed using a refractive index detector (RID).
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bioRxiv - Neuroscience 2021Quote: ... Endogenous peroxidase and phosphatase activity in the tissues was quenched with a dual enzyme block from Dako (S2003, Agilent, UK). Sections were washed in 0.1% PBS-T and blocked with the relevant serum (goat ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial respiration was examined in Chmp5Ctsk and wild-type skeletal progenitors using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent) as previously described53 ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA encoding full-length wild-type murine BimL was introduced into pBluescript II KS(+) vector (Stratagene, Santa Clara, CA). Sequences encoding a polyhistidine tag followed by a TEV protease recognition site (MHHHHHHGGSGGTGGSENLYFQGT ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Immunology 2023Quote: ... using 1 µg/mL of rat anti-mouse IgM (AbD Biotec) or rabbit anti-mouse IgG1 (Dako) capture antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Sections for brightfield microscopy were initially incubated with Dako dual enzyme block to quench endogenous peroxidase and alkaline phosphatase (AP) activity (Agilent Technologies) (15 min ...
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bioRxiv - Neuroscience 2023Quote: ... detected with polymeric alkaline phosphatase conjugated anti-rabbit IgG (BrightVision DPVR-AP, Immunologic) and visualized with stay red (DAKO, Glostrup, Denmark). Next ...
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Bioengineering 2019Quote: Variant CvFAPG462V was generated by site-directed mutagenesis of the wild-type construct in pETM11 using the QuikChange whole plasmid synthesis protocol (Stratagene) with CloneAmp HiFi PCR premix (Clontech) ...
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bioRxiv - Biophysics 2019Quote: ... anti-surfactant C protein antibody to target membrane antigen secreted from airway type 2 epithelial cells in alveoli or ii) anti-thyroid transcription factor-1 (TTF-1) antibody (Dako Agilent Products ...
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bioRxiv - Biophysics 2019Quote: ... anti-surfactant C protein antibody to target membrane antigen secreted from airway type 2 epithelial cells in alveoli or ii) anti-thyroid transcription factor-1 (TTF-1) antibody (Dako Agilent Products ...
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bioRxiv - Cell Biology 2019Quote: ... The Lys162Met variant for all AURKA biosensors analysed in the study were obtained from the corresponding wild-type constructs by QuikChange site-directed mutagenesis (Stratagene) with the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Immunology 2020Quote: ... These genes were cloned into an Adenovirus type 5 replication-defective E1/E3 deleted vector using the Ad-Easy Adenoviral Vector System (Agilent). Briefly ...
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bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
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bioRxiv - Genetics 2023Quote: ... The C211G mutant was generated by site-directed mutagenesis of the wild-type vector using the QuikChange II protocol (Agilent). To generate the Myc-tagged versions ...
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bioRxiv - Cell Biology 2023Quote: Basal oxygen consumption rates in kidneys from non-diabetic and diabetic wild-type and Angptl4-/- mice were characterized using the Seahorse Flux Analyzer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted metabolites were separated on a Cogent Diamond Hydride Type C column of 1200 liquid chromatography coupled to an Mass 6220 TOF spectrophotometer (Agilent). Ion abundances of metabolites were estimated using Profinder 8.0 and log2 fold changes were analyzed and plotted using Gene Cluster 3.0 and Java Treeview.
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bioRxiv - Biochemistry 2023Quote: Oxygen consumption rates (OCR) of wild-type C2C12 under various concentrations of uncouplers were measured with Seahorse XFe24 analyzer (Agilent). Experiments were conducted under the same process as explained above ...
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bioRxiv - Plant Biology 2019Quote: ... The resulting fatty acid methyl esters were analyzed using GC/MS (Agilent Technologies, Wilmington, DE) equipped with a capillary DB-23 column (30 m × 0.25 mm × 0.25 μm ...
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bioRxiv - Microbiology 2021Quote: ... Amino acids were separated using a VF-5ms inert 5% phenyl-methyl column (Agilent Technologies). The oven temperature was constant at 120 °C for 5 min ...
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bioRxiv - Neuroscience 2019Quote: ... Fatty acid separation was performed on an Infinity 1260 HPLC binary system (Agilent Technologies, France) equipped with a ZORBAX SB-C18 C18 50 × 2.1 mm 1.8 μm (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... B: Acetonitrile: Methanol − 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Physiology 2020Quote: The fatty acid oxidation (FAO) was measured using a microfluorimetric Seahorse XF96 Analyzer (Agilent Technologies) according to the protocol supplied by the manufacturer with minor modifications ...
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bioRxiv - Cell Biology 2023Quote: ... B: Acetonitrile: Methanol – 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Systems Biology 2024Quote: ... and amino acids was performed using gas chromatography-mass spectrometry (Agilent, GC-7890A, MS-5975C). Before the extraction of intracellular metabolites ...
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA quality and concentration of wild-type and mutant were determined using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA (~1 μg ...
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bioRxiv - Biophysics 2021Quote: ... An expression construct for R80A ParB was generated by site-directed mutagenesis of the wild type expression plasmid (QuikChangeII XL, Agilent Technologies). The mutant protein was expressed and purified in the same manner as wild type ...
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bioRxiv - Biochemistry 2022Quote: The L26P mutation was introduced into a wild-type AR sequence (pDONR221-AR-AD-WT) using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs:
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Biochemistry 2022Quote: ... A398P and T435P mutations were introduced step-wise into a wild-type AR sequence (pDONR221-AR-AD-WT) using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs:
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bioRxiv - Biochemistry 2022Quote: ... C238P mutations were introduced step-wise into a wild-type AR sequence encoded in pDONR221-AR-AD-WT using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs:
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bioRxiv - Genetics 2023Quote: ... we inserted a wild-type histone array sequence containing the 5 replication-dependent histone genes into a pBluescript II KS+ vector (Agilent #212207) and introduced mutations using a Q5 Site-Directed PCR Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: FRET-based analyses of DNA fork regression by SMARCAL1 in the presence of RPA and RAD52 (wild type or mutants) were carried out using a Cary Eclipse fluorescence spectrophotometer (Agilent technologies) at 30°C in buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were submitted to the Stanford Protein and Nucleic Acid Facility for quality assessment by Agilent Bioanalyzer to determine the RNA integrity (RIN ...