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Citations for Agilent :
51 - 100 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... A rat anti-FLAG monoclonal antibody (200474) was purchased from Agilent (Santa Clara, CA). MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I ...
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bioRxiv - Neuroscience 2023Quote: ... Primary α-DAT was detected by using secondary rabbit anti-rat (1;1,000; Dako, P0450). Upon visualization by using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences® ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was detected using a rat an,-Ki67 monoclonal an,body (Dako M7249) at a 1:25 dilu,on followed by bio,nylated goat an,-rat an,body secondary (Jackson ImmunoResearch 112-065-167 ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized to urea lysate protein levels and analyzed using Wave Desktop Software (Seahorse Bioscience), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
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bioRxiv - Molecular Biology 2023Quote: ... DA content in lysates was measured using high-performance liquid chromatography (HPLC) equipped with fluorometric detection (Agilent) and a Zorbax Eclipse Plus C18 column (125 × 4.6 mm ...
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bioRxiv - Genomics 2019Quote: ... and hybridized onto SurePrint G3 Rat GE 8 x 60K Microarrays v2 (AMADID 074036; Agilent Technologies) for 16-20 h at 65°C in an Agilent oven with rotisserie ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were incubated overnight at 4°C: rat anti-human CD31 (DAKO, M082329-2), rabbit anti-human S1PR1 (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... using 1 µg/mL of rat anti-mouse IgM (AbD Biotec) or rabbit anti-mouse IgG1 (Dako) capture antibody ...
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bioRxiv - Systems Biology 2019Quote: All lysates were subject to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described (Havugimana et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibodies used were goat anti-rabbit and rat anti-rabbit IgG-HRP (Dako, Santa Clara, CA, USA). Proteins were detected by chemiluminescence using the Clarity™ Western ECL Substrate coupled to a ChemiDoc XRS+(Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and incubated with biotinylated rabbit anti-rat IgG for 1h (1:200, DAKO Cytomation A/S, Denmark). Following avidin-biotin-peroxidase complex (Vector laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with TBST three times, membranes were exposed to appropriate secondary antibodies (anti-rabbit, anti-mouse, and anti-rat) coupled to HRP (Dako) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed and incubated with the corresponding secondary antibodies when needed (rabbit anti mouse, Abcam; rabbit anti rat, Vector; Goat anti-rabbit, Dako) and visualization systems (Bond Polymer Refine Detection ...
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bioRxiv - Cancer Biology 2023Quote: Dual indexed whole exome capture libraries were prepared from mouse and naked mole-rat skin biopsies and matched livers using SureSelect XT HS and XT Low Input Enzymatic Fragmentation protocol (Agilent). For mouse whole exome sequencing (WES ...
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bioRxiv - Immunology 2024Quote: ... In Nr4a1-/- and Nr4a1+/+ mouse thrombus sections neutrophils were visualized by anti-Ly6G (clone: 1A8; isotype: rat IgG2a) and MPO (rabbit polyclonal, #GA51161-2, DAKO; isotype ...
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bioRxiv - Immunology 2024Quote: ... In Nr4a1-/- and Nr4a1+/+ mouse thrombus sections neutrophils were visualized by anti-Ly6G (clone: 1A8; isotype: rat IgG2a) and MPO (rabbit polyclonal, #GA51161-2, DAKO; isotype: rabbit immunoglobulin fraction, DAKO). Alexa labeled secondary antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Physiology 2022Quote: ... the HRP-Anti-Rat IgG (MP-7444, Vector) for 45 min or the Goat Anti-Mouse Immunoglobulins/HRP (Dako-Agilent, P0447) at 1:100 for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed three consecutive times with Tris buffer (5min) and 1:10000 diluted donkey- or goat-anti-rat-HRP (Agilent Technologies) or 1:25000 diluted goat-anti-mouse-HRP (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... gondii cell lysate and enriched eluates from affinity beads using ion- exchange (IEX) liquid chromatography by an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) individually ...
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bioRxiv - Physiology 2022Quote: ... Immunologic), the HRP-Anti-Rat IgG (MP-7444, Vector) for 45 min or the Goat Anti-Mouse Immunoglobulins/HRP (Dako-Agilent, P0447) at 1:100 for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Immunology 2020Quote: ... 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h.
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bioRxiv - Neuroscience 2022Quote: ... Rat GluN2B-G689C-C1/2 and Rat GluN2B-G689S-C1/2 were generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent,Cat. # 200518). Primers for GluN2B-G689C Mutagenesis ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (both cell lines from Agilent). The expression of Q-eTud3 and Q-eTud5 was induced at A600 = 0.8 with 0.7 mM IPTG (isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2022Quote: ... C2C12 cells (1.0×104 cells) were seeded into XF96 Cell Culture Microplates (Agilent Technologies) coated with Matrigel and differentiated into myotubes ...
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bioRxiv - Biochemistry 2021Quote: ... 60,000 cells were plated on XF24 cell plates (Agilent) to measure cell respiration ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells (15,000 cells/well) were plated on an XF96 polystyrene cell culture microplate (Seahorse®, Agilent). The following day ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were plated at confluency (40,000 cells per well) in Seahorse XF96 Cell Culture Microplates (Agilent). Cells were transfected with siRNA or mammalian expression vectors at the time of plating (reverse transfection) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lifted and seeded at 2×104 cells/well in XFe96 Cell Culture Microplate (Agilent Technologies) in 80 μl of fresh medium ...
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bioRxiv - Immunology 2023Quote: Caco-2 cells were plated at 30,000 cells per well on Seahorse XFe24 Cell Culture Microplate (Agilent). At the end of the experiment ...
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bioRxiv - Biochemistry 2022Quote: ... cells were seeded at 10,000 cells/well in 96 well Seahorse XF cell culture plates (Agilent, UK). The sensor cartridges were hydrated overnight with tissue culture grade H2O in a non-CO2 incubator at 37°C as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto Seahorse XFe96 cell culture plate (Agilent) at a density of 6000 - 8000 cells/well with culture medium for 24 hours ...
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bioRxiv - Physiology 2022Quote: ... cells were seeded on XF96 cell culture microplates (Seahorse Bioscience) at a seeding density of 5.0 x 103 cells per well ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated in XF96 well cell culture microplates (Agilent) at equal densities in substrate-limited medium (DMEM with 0.5 mM glucose ...
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bioRxiv - Molecular Biology 2023Quote: After culturing cells on Seahorse XFe96 cell culture microplates (Agilent), the medium was replaced with Seahorse XF DMEM (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Agilent). Cells were grown to optical density at 600nm (OD600 ...
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bioRxiv - Cell Biology 2019Quote: AD293 cells (Agilent) were maintained in Dulbecco’s Modified Eagles medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (Stratagene) with an N-terminal TEV cleavable GST tag ...
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bioRxiv - Biophysics 2021Quote: ... AD293 cells (Agilent) were cultured in DMEM basic (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Agilent) were transformed with pET28-S plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Agilent) were transformed with ~2 μg αHL-D8H6 plasmid40 without heat shock and incubated on LB-agar plates containing 25 g L−1 lysogeny broth (LB ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Agilent). An overnight starter culture of cells was grown in 2xYT supplemented with carbenicillin (50 μg/L ...