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Citations for Agilent :
201 - 240 of 240 citations for RNase Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Detection was performed with anti-rabbit and anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibodies respectively (DakoCytomation, Carpinteria, CA, USA), followed by enhanced chemiluminescence (ECL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples were analyzed using an Agilent 1290 Infinity binary pump and autosampler with detection by an Agilent 6550 iFunnel QTof mass spectrometer (MS) (Agilent) using electrospray ionisation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Quantitative determination of diphenhydramine in human plasma was accomplished by the use of high-performance liquid chromatography with tandem mass spectrometry detection (Agilent Series 1100 LC with MS detector API4000 ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Microbiology 2022Quote: ... butyrate and propionate in microbial culture supernatants were quantified by gas chromatography with flame-ionization detection (GC-FID) (7890A, Agilent). For each sample and calibration standards ...
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bioRxiv - Immunology 2023Quote: For CFTR detection in cross sections, target retrieval was performed with a citrate buffer (Target Retrieval solution, pH 6, (Dako) for 30min after deparaffinization ...
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bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis was carried out utilizing a multiple reaction monitoring method for analyte detection on an Agilent LC-1290 Infinity II (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Mutations in genomic DNA from the spleen and liver were detected using the λ Select-cII Mutation Detection System for Big Blue Rodents (Stratagene) as we have done in the past (58).
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bioRxiv - Plant Biology 2021Quote: ... Extraction of total shikonins from growth media of hairy root cultures and quantification on an Agilent 1260 Infinity high performance liquid chromatography with diode array detection (HPLC-DAD) system (Agilent Technologies) was done as previously described 19 ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...
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bioRxiv - Physiology 2019Quote: ... Immunoblotting of 1-dimension SDS using COX7A2L antibody were analyzed by the ECL detection system using polyclonal goat anti-rabbit (Dako, P0448) as the secondary antibody.
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bioRxiv - Synthetic Biology 2023Quote: ... HPLC-PDA was performed using an Agilent 1200 system equipped with a G1315C SL diode array detection (DAD) module (Agilent, Germany). DAD spectrum scanning was from 210 nm to 550 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell plates were incubated with 10µl of Flash detection reagent (#93-0247, DiscoverX) for 30 min and chemiluminescence signals were detected with a microplate reader (Synergy MX, Agilent BioTek). Chemiluminescence data were normalized to the signal of basal vehicle-treated cells and mean chemiluminescence values were converted to concentration-response curves for determination of EC50 and Emax values (GraphPad Prism).
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bioRxiv - Neuroscience 2023Quote: ... 90 min and 180 min later and processed for blood ethanol concentration (BEC) determination by gas chromatography and flame ionization detection (Agilent 7820A).
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bioRxiv - Molecular Biology 2024Quote: ... to purify the library material and confirmed that the library DNA was in the size range of 200-800 bp using high sensitivity DNA Chip detection (Bioanalyzer 2100, Agilent Genomics), and sequenced the libraries on a NextSeq 500 instrument (Illumina ...
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bioRxiv - Physiology 2022Quote: ... The purified RNA pellet was diluted in 50 µL RNase-free water and served as template for cDNA synthesis (AccuScript™ High Fidelity 1st Strand cDNA Synthesis; Agilent Technologies, Santa Clara, USA). PCR amplification was conducted using Phusion® DNA Polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Samples were eluted in RNAse-free water and purified RNA was analyzed using Qubit RNA HS Assay Kit (Thermo) and Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) using the RNA High Sensitivity assay ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... The upper ether layer was collected and SCFA content was analyzed on an Agilent 7890B gas chromatography system with flame ionization detector using a high-resolution capillary column for detection of volatile acids (DB-FFAP, 30 m x 0.25 mm with 0.25 μm film thickness; Agilent Technologies, Santa Clara, CA). A standard solution containing 10 mM of acetic ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Developmental Biology 2020Quote: ... The slides were washed in PBS (0.05 M, pH 7.2) 3x and incubated with the secondary antibody (DAKO Detection System - K0690, USA). The slides were washed again in PBS and then incubated with the streptavidin-peroxidase (DAKO Detection System - K0690 ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+, Rabbit/Mouse Kit; Dako, Glostrup, Denmark). Mayer’s hematoxylin (Carl Roth ...
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bioRxiv - Physiology 2023Quote: ... High-resolution gas chromatography capillary column coated with 0.25µm film thickness was used (DB-FFAP) for the detection of volatile acids (Agilent Technologies, Santa Clara, CA). The oven temperature was 145°C and the FID and injection port were set to 225°C ...
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bioRxiv - Neuroscience 2023Quote: ... Liquid chromatography tandem mass spectrometric detection (LC-MS/MS) was performed on the 6495 Triple-Quadrupole LC/MS system (Agilent Technologies, Inc.) equipped with an ESI source ...
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bioRxiv - Biochemistry 2020Quote: ... and the VOCs species were further analyzed using a gas chromatograph-mass spectrometry/flame ionization detection (GC–MS/FID) system (Agilent Technologies, Inc., USA). A total of 117 VOC species were screened and quantified for all exposure tests using the GC-MS system ...
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bioRxiv - Genomics 2021Quote: The concentration of the substrate and the phosphorylated product at each time point was determined by reversed-phase HPLC with UV detection at 214 nm (Agilent 1260 Infinity II). A 40 μL volume of the quenched reaction was injected onto a C18 column (ZORBAX 300SB-C18 ...
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bioRxiv - Microbiology 2020Quote: ... the epitopes were unmasked by heating at 95°C for 30 min prior to detection on a Dako OMNIS™ automate (Agilent Technologies, USA). When indicated ...
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bioRxiv - Biochemistry 2021Quote: ... and 12 were used as primary detection against T22 capture antibody) was an anti-tau polyclonal rabbit antibody (A002401-2) purchased from Agilent (Santa Clara, CA). A goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of CH4 was quantified by gas chromatography with flame ionization detection (Hewlett Packard HP 5890 Series II Gas Chromatograph, Agilent Technologies, California, USA). Air pressure was also monitored at each sampling point by a portable pressure meter (GMH 3100 ...
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system; Dako, Nowy Sącz, Poland) for 1 hour and immunostaining was visualized with 3’3-diaminobenzidine (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... All genes were analyzed with the SYBR green detection method (PCR Biosystems, UK) using the Stratagene Mx3000P real[time QPCR system (Agilent Technologies, Santa Clara, USA). Gene expression data were normalized against housekeeping genes (Gapdh in primary osteoblasts and Atp5b in bone tissue ...
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bioRxiv - Cell Biology 2022Quote: Gene expression was analyzed by real time reverse transcriptase quantitative PCR (RT-qPCR) according to the Fast SYBR Green protocol with the AriaMx real-time PCR detection system (Agilent technologies, Santa Clara, CA). Primers were ordered from Invitrogen (ThermoFisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... an acetonitrile precipitation was performed prior to sample analysis by an Agilent 1290 Infinity II HPLC with an Agilent 6550 iFunnel Q-TOF mass spectrometer for detection (Agilent, Santa Clara, CA, USA). Gefitinib and deuterated gefitinib (gefitinib-d6 ...
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bioRxiv - Biochemistry 2024Quote: Gene expression was analyzed by real time reverse transcriptase quantitative PCR (RT-qPCR) according to the Fast SYBR Green protocol with the AriaMx real-time PCR detection system (Agilent technologies, Santa Clara, CA). Primers were ordered from Invitrogen (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: The detection of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) was determined using an XFe96 Extracellular flux bioanalyzer (Agilent technologies, Santa Clara, CA, USA) using a mitochondrial stress test ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: Ultra-high-performance liquid chromatography–diode array detection–quadrupole time-of-flight mass spectrometry (UHPLC–DAD–QTOFMS) was performed on an Agilent Infinity 1290 UHPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with a diode array detector ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...