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Citations for Agilent :
701 - 750 of 2715 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Immunohistochemistry (IHC) for SEMA4C was performed using a rabbit anti-SEMA4C primary antibody (#AF6125, R & D Systems) on an autostainer (Dako). Detection was achieved using the Envision rabbit detection system (Dako ...
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bioRxiv - Biochemistry 2019Quote: ... Assays were resolved by SDS-PAGE and results were analysed by western blotting with anti-ubiquitin primary antibody (Z0458, Dako) and anti-rabbit HRP secondary antibody for the unlabelled Ubc13~Ub conjugate ...
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bioRxiv - Physiology 2019Quote: ... at room temperature for 10 minutes and then with the primary antibodies diluted in Dako Antibody Diluent (Agilent Technologies, Inc.) overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... The primary antibodies sources and dilutions were as follows: rabbit poly-clonal anti-glia fibrillary acidic protein (GFAP; 1:500; DAKO), mouse monoclonal anti-Neuronal Class III B-Tubulin (TuJ1 ...
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bioRxiv - Biochemistry 2019Quote: ... we used horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulins for polyclonal antibodies or anti-mouse immunoglobulins for monoclonal primary antibodies from DAKO Diagnostika GmbH/Agilent Technologies (Hamburg ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were incubated with the appropriate primary antibody diluted in TNB (for podoplanin) or Antibody Diluent (cat # S0809, Dako) (for VEGF-D or LYVE 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
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bioRxiv - Microbiology 2021Quote: Immunofluorescent (IF) staining was performed using the following primary antibodies: polyclonal rabbit anti-HSV-1 (cross-reactive with HSV-2; Agilent), anti-Iba1 (Wako ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary antibody overnight and then with horseradish peroxidase (HRP)-conjugated secondary antibodies (Agilent Technologies, Stockport, UK) for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked with 5% normal donkey serum for 1 h at room temperature and incubated with primary antibodies (rabbit anti-GFAP 1:200, Dako; goat anti-Iba1 ...
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bioRxiv - Neuroscience 2023Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in primary cultures of DRG neurons using the Seahorse XFp analyzer (Agilent). DRG neurons were cultured (1 ...
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bioRxiv - Microbiology 2023Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were permeabilized with 0.2% Triton X-100 in 2% fish gel for 2 hours at room temperature and immunohistochemically labelled with the primary antibody (1:200 rabbit anti-GFAP, Z0334 Dako; 1:200 rabbit anti-Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody detection was achieved through secondary peroxidase/DAB+ staining using the Dako REAL EnVision Detection System (Agilent, DAKO, #K5007).
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody detection was achieved through secondary peroxidase/DAB+ staining using the Dako REAL EnVision Detection System (Agilent, DAKO, #K5007).
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrieval buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3% ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were then incubated at room temperature for 60 min in primary antibodies diluted with antibody diluent (Dako Omnis). The following primary antibodies against various human differentiation antigens were used ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Microbiology 2023Quote: ... Blocking solution was washed out with PBS containing 0.05% Triton X-100 and samples were stained with primary antibody diluted in Dako antibody diluent (Agilent Technologies). Primary antibodies and dilutions used were as follows ...
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bioRxiv - Pathology 2023Quote: ... the blocking solution was shaken off and the primary antibodies Arginase (Arg1 biocare cat#ACI3058) and CD68 (Dako cat#M0814) were applied ...
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bioRxiv - Developmental Biology 2023Quote: ... After permeabilization and blocking primary antibody solution using sheep anti Dll1 (Catalog no. AF3970, RnD Systems) and rabbit anti Lysozyme (Catalog no. A0099, Dako) antibodies at a concentration of 1:100 in PBS supplemented with 3 % donkey serum and 0.1 % TritonX was added ...
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genomics 2019Quote: ... 37,608 individual biotinylated RNAs targeting the ends of 22,076 promoter-containing human HindIII restriction fragments) were prepared according to the manufacturer’s instructions (SureSelect Target Enrichment, Agilent Technologies). The Hi-C library DNA was denatured for 5 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Genetics 2019Quote: ... DNA libraries were prepared by using the SureSelect Human All Exon V6 kit (Agilent Technologies, Santa Clara, CA, USA) and were paired-end sequenced on the Illumina HiSeq 4000 platform at 100X coverage ...
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bioRxiv - Genetics 2021Quote: Oxford Nanopore MinION long-read sequencing was performed on RNA isolated from an adult human cortex (540005, Agilent Technologies). PolyA-enriched RNA was isolated using the Dynabeads mRNA Direct kit (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome-sequencing libraries were generated using the Agilent SureSelect Human All Exon V4+UTR capture platform (Agilent, CA, USA). Libraries were sequenced to a target depth of 200-fold coverage on a 100 bp paired-end sequencing run using an Illumina HiSeq 2000 instrument ...
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bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...
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bioRxiv - Neuroscience 2021Quote: Oxygen Consumption Rate (OCR) from live human iPSC-derived neurons was measured using a Seahorse XFe-96 Analyzer (Agilent). 30,000 human pre-differentiated neurons were seeded per well in Poly-D-Lysine + Laminin-coated 96-well Seahorse plate and differentiated for the indicated time (2 or 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: The tumor and normal adjacent samples were prepared using SureSelect Human All Exon V7 library (Agilent, Santa Clara USA). 100 bp paired end reads sequencing was performed using the Illumina NovaSeq 6000 system by Edinburgh Genomics (Edinburgh ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Genetics 2020Quote: ... enrichment and elution were performed according to manufacturer’s instructions and protocols (SureSelect Human All Exon Kits Version CRE, Agilent). The library was sequenced on an Illumina HiSEQ 2500 as paired-end 75bp reads ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor mutational burdens were computed using the exonic ranges from the SureSelect Human All Exon v7 panel from Agilent (bed file downloaded from the manufacturer’s website ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: AMACR/p63 immunohistochemistry (IHC) double staining (Monoclonal Rabbit Anti-Human AMACR, clone 13H4, Dako, Code M361601-2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SCA-associated mutations were introduced into human cMyc-mGlu1 using Quikchange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis primers are shown in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Immunology 2024Quote: ... Microarray analysis was performed at TaKaRa-Bio using high-density oligonucleotide array (Human Genome U133 Plus 2.0) and GeneSpring 12.5 software (Agilent Technologies) as described previously (17) ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% TritonX-100 for 1 hour at room temperature and then incubated overnight at 4°C with the primary antibodies rabbit anti-Iba-1 (Wako: 019-19741, RRID:AB_839504) at 1:500 or rabbit anti-GFAP (Dako: Z0334, RRID: AB_10013382) at 1:1000 and then secondary antibodies (Alexa goat-647 anti-Rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...