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Citations for Agilent :
1 - 50 of 122 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Hepatocytes were then cultured in collagen type I coated XF24 cell culture microplates (Seahorse Bioscience) at 1.5 ×104 cells per well and incubated 4-6 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... collagen type IV (2 μg /ml clone M0785, Dako, Agilent Pathology Solutions ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Bioengineering 2022Quote: ... collagen type IV (2 μg /ml clone M0785, Dako, Agilent Pathology Solutions, Santa Clara, CA), fibronectin (2 μg/ml clone MAB1918 ...
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bioRxiv - Immunology 2020Quote: ... Collagen IV (Dako/M0785), Tenascin (Chemicon/MAB19101) ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... A single C4 Type A (Agilent, G9203) SPE cartridge was used for the sample clean-up ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I using GeneJammer (Agilent, 204130) transfection reagent ...
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bioRxiv - Immunology 2020Quote: ... Mutations of W169G on ASCCARD (Type IIa) and G20K on caspase-1CARD (Type IIb) were performed by QuikChange Mutagenesis (Agilent Technologies). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... wild type human TTC3 in pCMV-Tag2 vector (Stratagene), mouse AUTS2-myc-DDK in pCMV6 (OriGene ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I using GeneJammer transfection reagent (Agilent technologies, 204131). After 24 h of incubation ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I using GeneJammer transfection reagent (Agilent technologies, 204131). After 24 h of incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... preparative HPLC (Agilent Technologies 1260 Infinity I/II) was conducted to separate excess ampicillin from PEG-tetra-ampicillin ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The samples were loaded onto a C18 Type SPE cartridge (Agilent) using deionized water containing 0.1% trifluoracetic [TFA] at flow rate of 1.5 mL/min and eluted to the mass spectrometer using acetonitrile/deionized water (95/5 ...
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bioRxiv - Physiology 2022Quote: ... and a Masson’s Trichrome Stain Kit to identify collagen fibers and fibrin (AR17311-2, Artisan, Dako, Agilent). Stainings were performed in a Dako Autostainer Plus (Dako ...
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bioRxiv - Physiology 2022Quote: ... and a Masson’s Trichrome Stain Kit to identify collagen fibers and fibrin (AR17311-2, Artisan, Dako, Agilent). Stainings were performed in a Dako Autostainer Plus (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... A graphite C18/type D solid phase extraction (SPE) cartridge (Agilent Technologies) was used for analyte/IS adsorption/elution ...
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bioRxiv - Biophysics 2023Quote: The kinetics study of collagen self assembly was performed on a UV-Vis spectrophotometer (Agilent Technologies, Cary 8453). Neutralized cold collagen solutions were pipetted into plastic cuvettes (Brand ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 peptide-loaded HLA class I tetramers were generated by conjugation of biotinylated peptide-loaded HLA class I easYmers® with phycoerythrin (PE)-conjugated streptavidin (Agilent, Santa Clara, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: I used the QuikChange™ site-directed mutagenesis method (Agilent Technologies), modified to use a single primer and 35 cycles of amplification ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
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bioRxiv - Developmental Biology 2019Quote: ... as it has been previously established to be appropriate for most cell types (Agilent Seahorse XF Cell Mito Stress Test Kit User Guide ...
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bioRxiv - Genetics 2021Quote: ... we used the i) Agilent SureSelectXT Human All Exon V6 (Agilent Technologies), ii ...
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bioRxiv - Physiology 2023Quote: ... The DNA plasmids were treated with Dpn I restriction enzyme (Agilent Technologies) to digest the methylated parental DNA template and subsequently transformed into XL10-Gold competent cells (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... using three liquid chromatography methods applied to two distinct types of mass spectrometry instruments (Agilent qTOF 6545 ...
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bioRxiv - Microbiology 2021Quote: ... and type 1 IFN response (Mx1) was performed with MPO (Dako Cat. No. A0398; polyclonal) at 1:1000 detection using Rabbit Polink-1 HRP ...
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bioRxiv - Genetics 2023Quote: ... was used with a polyethylene glycol (PEG) type polar phase column (HP-Innowax, ref. Agilent 19091N-116I ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Immunology 2021Quote: ... and 0.5μM rotenone/antimycin A (mitochondrial complex I and III inhibitors; Agilent Technologies). After injection of oligomycin A ...
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bioRxiv - Genetics 2022Quote: ... Purified DNA and RNA was then treated with DNAse I (Agilent cat. 600031) to remove contaminating DNA from each sample ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Mutations in the wild type heavy chain plasmid were performed using site directed mutagenesis (Agilent, 200524) following the manufacturer’s protocol to generate EG ...
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bioRxiv - Physiology 2019Quote: Tendon fibroblasts were treated for 48 hours prior to being plated (8,000 cells) into collagen coated XFp cell culture miniplates (Seahorse Bioscience, Agilent, Santa Clara, CA). Cultured cells from each treatment condition were reseeded in duplicate ...
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bioRxiv - Genomics 2020Quote: ... Quality control of DNase I-treated RNA was performed with Bioanalyzer 2100 (Agilent Technologies), which yielded the RIN values of 8.7 and 9.1 for the respective tissues ...
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bioRxiv - Microbiology 2020Quote: ... according to the following equation (I): using Varian Cary Eclipse fluorescence spectrophotometer (Agilent Technologies). The relative ΔGP is obtained by subtracting the GP value in the absence of flagella from all GP values ...
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bioRxiv - Physiology 2019Quote: Tendon fibroblasts were treated for 48 hours prior to being plated (8,000 cells) into collagen coated XFp cell culture miniplates (Seahorse Bioscience, Agilent, Santa Clara, CA). Cultured cells from each treatment condition were reseeded in duplicate ...
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bioRxiv - Physiology 2022Quote: ... Extracted collagen was derivatized for analysis of deuterium enrichment of alanine using Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA). To determine the precursor pool enrichment ...
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bioRxiv - Biochemistry 2020Quote: Wild-type (WT) and F94S His6-CSAD were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL cells (Stratagene) at +15 °C using 0.5 mM IPTG induction ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Genetics 2020Quote: ... Mutations were introduced by site-directed mutagenesis of the wild-type gene using the QuikChange mutagenesis kit (Agilent). Sequencing was performed to confirm correct base pair substitutions or deletions were made.
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bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial respiration was examined in Chmp5Ctsk and wild-type skeletal progenitors using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent) as previously described53 ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA encoding full-length wild-type murine BimL was introduced into pBluescript II KS(+) vector (Stratagene, Santa Clara, CA). Sequences encoding a polyhistidine tag followed by a TEV protease recognition site (MHHHHHHGGSGGTGGSENLYFQGT ...
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bioRxiv - Molecular Biology 2019Quote: ... Mutant versions of JMJD6 were generated from the wild type construct using QuikChange Lightning site-directed mutagenesis kit (Agilent). Amino and carboxy terminal truncations of JMJD6 were generated by PCR amplification ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Bioengineering 2022Quote: ... and i-caproate were measured using a 1100 series HPLC-RID/UV system (Agilent Technologies, Germany) equipped with pre-column and column Rezex ROA-Organic Acid H+ (8% ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Quality control of the RNA treated with DNase I was performed with Bioanalyzer 2100 (Agilent Technologies). Libraries were prepared with TruSeq RNA Sample Prep Kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Samples were treated with DNAse I and were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies). To improve the quality of the readings ...
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...