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Citations for Agilent :
251 - 300 of 360 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody in TBS-T (1:3000, Dako, Glostrup, Denmark) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells (0.2 x 106/well) were counted and plated in XF RPMI medium (pH 7.4, Agilent 103576) containing 10mM glucose (103577 ...
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bioRxiv - Systems Biology 2020Quote: ... lyophilized samples were resuspended in Buffer A (5 mM NH4HCO2/ 2% ACN) and 5 mg peptide material (5 mg/ml) was loaded onto a reversed phase column (ZORBAX 300Extend-C18, Agilent). Peptides were separate at a flow rate of 2 ml/min and a constant column temperature of 40 °C using a binary buffer system ...
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bioRxiv - Cell Biology 2021Quote: Ap-proximately 1000 ng of peptides from each biological replicate before SCX fractionation were first injected onto an Agilent 1290 Infinity UHPLC system (Agilent) on a 50-cm analytical column packed with C18 beads (Dr Maisch Reprosil C18 ...
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bioRxiv - Genomics 2022Quote: ... The total peptide concentration was measured by UV absorbance at 210 nm using an LC system with UV detector (Agilent), with roughly 10 mg tissue yielding 500-750 μg peptide mixture.
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bioRxiv - Bioengineering 2021Quote: Peptides used for pMHC refolding were synthesized in housed using the Liberty Blue Peptide Synthesizer (CEM) and validated using LC-MS (Agilent). To generate pMHC molecules for bioconjugation ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 200 µg of dried peptides were subjected to phosphopeptide enrichment using IMAC cartridges and a BRAVO AssayMAP liquid handling platform (Agilent) as recently described 47.
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Biochemistry 2022Quote: Purity of the wild-type or mutant FAM237A peptides was analyzed by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technologies). The FAM237A peptides were eluted from the C18 reverse-phase column by an alkaline acetonitrile gradient composed of alkaline solvent A and B [15] ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide fractions were eluted from a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm; Agilent Technologies) by an acetonitrile gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... at 200 µl min-1 and the digested peptides were captured on a 2 mm x 1 cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptides were then purified and concentrated using OMIX Tips C18 reversed-phase resin (Agilent Technologies, Santa Clara, CA, USA). The peptides were dehydrated in a vacuum centrifuge and analysed by NanoLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: Fluorophore-conjugated (or biotinylated) peptides were purified via reversed phase chromatography on a C18 column (Pursuit XRs C18 column, Agilent) to separate peptide-dye conjugates from unconjugated peptides or dyes ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using a modified in-solution sample preparation protocol.94 One hundred micrograms of desalted peptides were fractioned on an microflow HPLC (1260, Agilent) using a XBridge peptide BEH C18 column (130 Å ...
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bioRxiv - Systems Biology 2023Quote: ... equivalent amounts of each digested sample were pooled before proceeding to a peptide fractionation using a manual workflow based on Stage-Tips or an automatic workflow with the AssayMAP Bravo (Agilent).
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg of TMT-labeled peptides were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Plant Biology 2023Quote: ... and portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system ...
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bioRxiv - Microbiology 2023Quote: ... Solid phase extraction of peptides was done using equilibrated C18 pipette tips (Bond Elut OMIX 100 µl pipette tips, Agilent). The samples are aspirated for 10 cycles for maximum binding efficiency and then eluted in LC-MS/MS vials with 100 µl of 0.1% TFA in water:acetonitrile (at a ratio of 30:70 (v/v)) ...
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Cancer Biology 2024Quote: ... enrichment of phosphorylated peptides was performed on the Bravo automated liquid handling platform using AssayMAP Fe(III)-NTA cartridges (Agilent). In brief ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The membranes were washed with TBS-T and incubated with corresponding HRP conjugated secondary antibody (Agilent Dako, Stockport, UK) for 1h at room temperature ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The membranes were washed with TBS-T and incubated with corresponding HRP conjugated secondary antibody (Agilent Dako, Stockport, UK) for 1h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
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bioRxiv - Microbiology 2021Quote: ... and quantification of their tertiary butyldimethylsisyl (t-BDMS) derivatives using capillary GC apparatus (Agilent 6890; Agilent Technologies, Santa Clara). Two technical replicates of an external standard (acetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... The blots were then washed 3x with 0.1% PBS-T and incubated with the relevant horseradish peroxidase (HRP)-conjugated secondary antibody (Dako) diluted 1:2000 in 5% milk/PBST ...
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bioRxiv - Genetics 2019Quote: ... and AFF4 NM_014423.4:c.772C>T mutations were engineered using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... Slides were again washed in TBS-T two times followed by incubation with anti-rabbit EnVision detection kit (DAKO) for 1 hour in room temperature ...
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bioRxiv - Immunology 2024Quote: T cell oxygen consumption rate (OCR) was measured using a Seahorse XFe96 Extracellular Flux Analyzed following established protocols (Agilent). Briefly ...
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bioRxiv - Immunology 2024Quote: ... 400,000 T cells/well were plated onto Poly-D-lysine coated cell culture plate in RPMI XF media (Agilent) supplemented with 10mM glucose (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...
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bioRxiv - Immunology 2022Quote: ... samples were acidified with formic acid (FA) and subsequently peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo Platform (Agilent Technologies). Two biological replicates were pooled together to generate 100 micrograms of total peptide for TMT labeling and subsequent fractionation and phosphopeptide enrichment ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μl of each sample containing 2 μg of total native peptides and 100 fmol of each standard peptide was loaded on a precolumn Zorbax 300SB-C18 (5 μm, 5 × 0.3 mm) (Agilent Technologies, USA) and washed with 5 % acetonitrile for 5 min at a flow rate of 10 μl/minute before analytical LC separation ...
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bioRxiv - Systems Biology 2020Quote: ... 3 μl of each sample containing approximately 10 μg of total native peptides and 500 fmol of each stable isotope-labeled standard (SIS) peptide were loaded on an analytical column, Zorbax 300SB-C18 (5 μm, 150 × 0.3 mm) (Agilent Technologies, USA) and washed with 5% acetonitrile for 5 min at a flow rate of 20 μl/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Cell Biology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Biophysics 2020Quote: ... Peptides were trapped in a trap column (OPTI-TRAP for peptides, Optimize Technologies) and separated on a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies) using a 27 min gradient of 8-40 % organic solvent (0.4 % formic acid in acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Cancer Biology 2019Quote: 68Ga-labeled peptide was separated from non-radioactive NAPamide by reverse phase HPLC on an Agilent 1200 system (Agilent, Waldbronn, Germany). The complete mixture from the reaction chamber was loaded on an Eclipse XDB-C18 bonded silica column (Agilent ...
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bioRxiv - Physiology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18, 3.5 μm, Agilent Technologies, USA) at a flow rate of 300 nL/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Immunology 2023Quote: ... These SCX fractions (n = 5) were cleaned up using C18 spin columns (Peptide Cleanup Spin Tubes, Agilent Technologies, USA, #5188-2750) and dried using SpeedVac at 22 °C.
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bioRxiv - Synthetic Biology 2022Quote: ... The peptides were purified by reverse-phase (RP)-HPLC using a preparative Agilent 1260 Infinity II Series HPLC-system (Agilent Technologies) with column 1 (Supplementary Table 13) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 10% formic acid (FA) and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...