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Citations for Agilent :
1 - 50 of 2124 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina, PT0017_Covaris_20ng_Illumina, Agilent_UHR_20ng_Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-IgL (Clone: N/A; 1:400; Agilent; #GA507), and anti-IgK (Clone ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-IgK (Clone: N/A; 1:4000; Agilent; #A0191). Optimization of the antibodies and staining conditions was carried out on sections of human tonsil ...
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bioRxiv - Biochemistry 2024Quote: ... were used to clone full-length genes into pESC-HIS (GenBank: AF063850.1, Agilent) vector for yeast expression and 3Ω1 (Sarrion-Perdigones et al. ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was performed using Accuscript Hi Fidelity First Strand Synthesis kit (Agilent). The amount of RNA entered into the reaction was normalised between samples ...
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bioRxiv - Pathology 2020Quote: ... Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Cell Biology 2020Quote: ... coli-optimized synthetic gene encoding the E2 ORF from HPV-16 (GenBank: AAD33255.1) was cloned into pCAL-N-FLAG (Agilent Technologies, CA, USA), which contains a vector-encoded N-terminal calmodulin bind site (CBS) ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-p21waf1 (Clone SX118, Dako), Anti-Giantin antibody [9B6] - cis/medial Golgi Marker (ab37266 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti- p21 (Clone SX118, Dako) primary antibody was diluted in 100μl PBS-BT at 1/200 and incubated with cells at ambient temperature for 1 h ...
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bioRxiv - Pathology 2020Quote: ... Abcam, Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
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bioRxiv - Molecular Biology 2021Quote: ... The PGBD1 ORF was amplified from a cDNA library prepared from HeLa cells using DNA polymerase Pfu Ultra II Fusion HS (Agilent Technologies) and PGBD1 Forw (5’-ATGTATGAAGCTTTGCCAGGC-3’ ...
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bioRxiv - Microbiology 2024Quote: ... human cGAS was expressed as a fusion protein with a 6×His-SUMO2 tag in Escherichia coli (E. coli) BL21-RIL DE3 (Agilent) bacteria harboring a pRARE2 tRNA plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... PARLSkd3 and variants were expressed with an N-terminal MBP-tag in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in lysis buffer (40 mM HEPES-KOH pH = 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-PCNA Clone PC10 (Dako, M0879) at 1:500 following antigen retrieval ...
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bioRxiv - Immunology 2023Quote: ... mouse α-human CD20 (clone L26, Dako), rabbit α-CD3 (SP7 ...
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bioRxiv - Neuroscience 2024Quote: ... monoclonal Mouse anti-Synaptophysin clone SY38 (DAKO) 1:500 ...
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bioRxiv - Immunology 2020Quote: ... and α-CD19 (clone HD37; Dako [α-human]; clone SJ25-C1 [α-mouse]) were added to 5 × 108 cells for 5 min at 37° ...
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bioRxiv - Bioengineering 2022Quote: ... human CD31 (mouse, 1:200, clone JC70A, Agilent), mouse CD31 (rat ...
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bioRxiv - Pathology 2023Quote: ... the monoclonal mouse antibody (M7047, Clone 1D5, DAKO) was incubated for 30 minutes at room temperature at a 1∶50 dilution ...
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bioRxiv - Pathology 2024Quote: ... CD34 (mouse monoclonal antibody, clone QBEnd10; Dako/Agilent), α-smooth muscle actin (SMA ...
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bioRxiv - Pathology 2024Quote: ... SMA (mouse monoclonal antibody, clone 1A4; Dako/Agilent), CD163 (mouse monoclonal antibody ...
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bioRxiv - Pathology 2024Quote: ... SMA (mouse monoclonal antibody, clone 1A4; Dako/Agilent), CD163 (mouse monoclonal antibody ...
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bioRxiv - Pathology 2024Quote: ... CD34 (mouse monoclonal antibody, clone QBEnd10; Dako/Agilent), α-smooth muscle actin (SMA ...
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bioRxiv - Immunology 2022Quote: ... CD68 (primary mouse IgG1, clone KP-1, DAKO), CD20-eF615 (clone L26 ...
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bioRxiv - Microbiology 2022Quote: ... HRP-labelled goat anti-mouse immunoglobulin (cat. n° P0447, Dako). The following antibodies were used for flow cytometry ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-CD44 (1:50, clone DF1485, Dako, M7082), rabbit anti-EGFR (prediluted ...
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bioRxiv - Neuroscience 2020Quote: ... mouse clone CR3/43 (1:20, Agilent, M077501–2). Sections were incubated for 1 hour at room temperature with secondary antibodies (1:1000) ...
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bioRxiv - Molecular Biology 2021Quote: ... and mouse anti-HIV p24 (Kal-1 clone, Dako). DAB staining was performed using a Ventana Ultraview DAB detection kit in a Ventana BenchMark XT processor (Ventana Medical Systems ...
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bioRxiv - Physiology 2023Quote: ... CD68 (mouse monoclonal, 1:100, M0814 KP1 clone, Dako), GFAP (mouse monoclonal ...
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bioRxiv - Pathology 2023Quote: ... the PgR monoclonal mouse antibody (M3569, clone 636, DAKO) was incubated 30 minutes at room temperature at a 1:150 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... mouse monoclonal anti-GFAP (clone 6F2, Dako Cat#M0761), and recombinant rabbit monoclonal anti-LUM (Lumican ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako ...
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bioRxiv - Pathology 2024Quote: ... and CD34 (mouse monoclonal antibody, clone QBEnd10; Dako/Agilent). Next ...
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bioRxiv - Pathology 2024Quote: ... and CD34 (mouse monoclonal antibody, clone QBEnd10; Dako/Agilent). Next ...
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bioRxiv - Microbiology 2022Quote: ... anti-CD20 (mouse monoclonal [Clone L26]; Agilent; 1:200); anti-VGLUT1 (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2024Quote: Primary antibodies: mouse anti-human CD20 (clone L26, Dako); rabbit anti-human CD3 (clone SP7 ...
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bioRxiv - Microbiology 2022Quote: ... HRP-labelled goat anti-mouse immunoglobulin (IgG; cat. n° P0447, Dako). The following antibodies were used for flow cytometry ...
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bioRxiv - Biochemistry 2020Quote: ... Salmonella typhimurium MsbA (T561C in a C88A/C315A cysteine-less background) with an N-terminal poly-histidine-tag was expressed in BL21-CodonPlus (DE3)-RIPL (Agilent). Expression was induced with 1 mM IPTG for 4 hours at 30°C and membrane proteins were solubilized with 1% DDM and 0.04% sodium cholate in a buffer containing 100 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+) using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) with oligonucleotides 5-N-His-UPF1 and 5-N-HIS-UPF1-r to yield pGEM3Zf(+)-6XHis-UPF1 ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...