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Citations for Agilent :
451 - 500 of 732 citations for PB Cadherin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were permeabilized with 0.1% Triton x-100 (Fluka, 93426) and blocked with the blocking solution containing 3% Goat serum (Dako, Cat. no. X0907), 0,1% sodium azide (the healthcare business of Merck KGaA ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...
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bioRxiv - Immunology 2022Quote: ... samples were acidified with formic acid (FA) and subsequently peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo Platform (Agilent Technologies). Two biological replicates were pooled together to generate 100 micrograms of total peptide for TMT labeling and subsequent fractionation and phosphopeptide enrichment ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μl of each sample containing 2 μg of total native peptides and 100 fmol of each standard peptide was loaded on a precolumn Zorbax 300SB-C18 (5 μm, 5 × 0.3 mm) (Agilent Technologies, USA) and washed with 5 % acetonitrile for 5 min at a flow rate of 10 μl/minute before analytical LC separation ...
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bioRxiv - Systems Biology 2020Quote: ... 3 μl of each sample containing approximately 10 μg of total native peptides and 500 fmol of each stable isotope-labeled standard (SIS) peptide were loaded on an analytical column, Zorbax 300SB-C18 (5 μm, 150 × 0.3 mm) (Agilent Technologies, USA) and washed with 5% acetonitrile for 5 min at a flow rate of 20 μl/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Cell Biology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Biophysics 2020Quote: ... Peptides were trapped in a trap column (OPTI-TRAP for peptides, Optimize Technologies) and separated on a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies) using a 27 min gradient of 8-40 % organic solvent (0.4 % formic acid in acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Cancer Biology 2019Quote: 68Ga-labeled peptide was separated from non-radioactive NAPamide by reverse phase HPLC on an Agilent 1200 system (Agilent, Waldbronn, Germany). The complete mixture from the reaction chamber was loaded on an Eclipse XDB-C18 bonded silica column (Agilent ...
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bioRxiv - Physiology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18, 3.5 μm, Agilent Technologies, USA) at a flow rate of 300 nL/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Immunology 2023Quote: ... These SCX fractions (n = 5) were cleaned up using C18 spin columns (Peptide Cleanup Spin Tubes, Agilent Technologies, USA, #5188-2750) and dried using SpeedVac at 22 °C.
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bioRxiv - Synthetic Biology 2022Quote: ... The peptides were purified by reverse-phase (RP)-HPLC using a preparative Agilent 1260 Infinity II Series HPLC-system (Agilent Technologies) with column 1 (Supplementary Table 13) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 10% formic acid (FA) and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Biophysics 2024Quote: ... Later the peptide was purified using reverse-phase high- performance liquid chromatography (HPLC) using C18 column in an HPLC instrument (Agilent Technologies). The pure peptide was lyophilized from water and was characterized using matrix-assisted laser desorption/ionization mass spectrometry.
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight on a shaker at room temperature in fresh blocking solution with polyclonal rabbit anti-GFAP (1:1000, Dako, Santa Clara, CA). The following day tissue was washed in PBST and incubated for 2.5h at room temperature with Alexa Fluor 488 donkey anti-rabbit secondary (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... grown cells were blocked with 1x PBS containing 3% goat serum (DAKO) for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed with PBS and afterwards mounted with the fluorescent mounting medium (Dako).
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bioRxiv - Physiology 2019Quote: ... PBS-washed sections were blocked with 5% goat serum (Dako, Hamburg, Germany) prior to primary antibody incubation ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBS and incubated with rabbit linker (DAKO, #K8019) followed by secondary antibody in 1% BSA/PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were washed with PBS and blocked with Dako protein block (Agilent) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed with 1x PBS using a microplate washer (405LS, Agilent). Cells were then fixed with 4% Formaldehyde and stained with DAPI (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were mounted after rinse in PBS with mounting medium (Agilent Dako), dried overnight at RT and stored at −20 °C until imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were mounted after rinse in PBS with mounting medium (Agilent Dako), dried overnight at RT and stored at −20 °C until imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... onto a peptide trap (Zorbax 300 SB-C18, 0.3 i.d. × 5 mm, 5 µm, 300 Å; Agilent Technologies, Santa Clara, CA, USA) for concentration and desalting with a pump running in isocratic mode with 0.1% formic acid in water ...
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bioRxiv - Biochemistry 2020Quote: ... Protein or peptide (fragments of peptide) intact mass was acquired using an MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc.). Data analysis was performed using MassHunter Qualitative Analysis Version B.07.00 (Agilent ...
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bioRxiv - Systems Biology 2020Quote: 200 ng samples of peptides were separated in a high performance liquid chromatography system (1260 Infinity LC System, Agilent, Santa Clara, CA) and a C18 column (3.5 μm particle size ...
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bioRxiv - Microbiology 2020Quote: Two different glycosidases were used for specific removal of N-glycans: peptide N-glycosidase F (PNGase F) and endo N-glycosidase H (Endo H) (Prozyme, Hayward, CA). PNGase F removes all N-glycans from gp120/41 glycoprotein whereas Endo H removes high-mannose and hybrid glycans ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pooled TMT-labeled peptide sample was fractionated using basic pH reverse-phase liquid chromatography with an Agilent 1260 Infinity HPLC system (Agilent, CA, USA). The chromatography conditions were as follows ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Synthetic Biology 2019Quote: Peptides were analyzed using an Agilent 1290 liquid chromatography system coupled to an Agilent 6460QQQ mass spectrometer (Agilent Technologies, Santa Clara, CA) operating in MRM mode ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were washed with PBS before the addition of the secondary antibody (DAKO) and incubated at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilised (0.1% TritonX-100 in PBS) and blocked (Serum free protein block, DAKO), before overnight incubation with a rabbit polyclonal antibody to mouse CD31 (ab28364 ...
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bioRxiv - Cell Biology 2022Quote: ... in 0.1% Triton 1x PBS] and Dako Fluorescent Mounting Medium (Dako, Cat S3023). Images were acquired on Zeiss LSM 700 laser scanning microscope.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with 0.01M PBS and mounted on glass slides (Dako, Denmark) and left to air dry for approximately 10 minutes before being coverslipped (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... washed 3×10 minutes with PBS again and mounted using glycergel (Dako, Cat.N.C0563). The imaging was performed with the use of ZEISS microscopes ...
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bioRxiv - Cell Biology 2023Quote: ... we equipped the pBluescript vector (pBS SK+, Agilent technologies, Santa Clara, CA, USA) with the murine U6 promotor controlled sgRNA expression cassette from pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with PBS before mounting with Fluorescent Mounting medium (Dako).
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bioRxiv - Neuroscience 2023Quote: ... Samples were washed in PBS and mounted using DAKO fluorescence mounting medium (Agilent).
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bioRxiv - Genetics 2023Quote: ... Slides were washed once in PBS before mounting with DAKO mounting media (Agilent).
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bioRxiv - Synthetic Biology 2019Quote: ... Peptides prepared for shotgun proteomic experiments were analyzed by using an Agilent 6550 iFunnel Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to an Agilent 1290 UHPLC system as described previously [36] ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were then analysed by liquid chromatography mass spectrometry (LC-MS) using an Agilent 1290 Infinity HPLC (Agilent Technologies, Santa Clara, CA, USA) connected to a Xevo G2-XS QTof mass spectrometer (Waters ...