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Citations for Agilent :
151 - 200 of 2967 citations for Nectin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Universal Human Reference RNA (740000, Agilent Technologies) and Human Brain Reference RNA (QS0611 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-4 μm slides were cut and stained for MLH1 (Monoclonal Mouse Anti-Human; Clone ES05, dilution 1 : 10,Dako), MSH2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Physiology 2020Quote: ... 20 μL samples were injected into Hi-Plex H column (300×7.7 mm; 8 μm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells were screened accordingly to the method for human DCCs using immunofluorescent staining of the cell suspension with anti-human EpCAM (Ber-EP4-FITC, Agilent, or HEA-125-PE ...
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bioRxiv - Genomics 2020Quote: The Universal Human Reference RNA was from Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... rabbit antibody against anti-human MPO (A0398, Dako), mouse β actin (A1978 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies against human tau (DAKO Cat# A0024) and β-actin (Cell Signaling Cat# 3700 ...
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bioRxiv - Immunology 2020Quote: ... mouse-anti-human EGFR (Dako, DAK-H1-WT), and CLTX-biotin ...
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bioRxiv - Genomics 2019Quote: ... Library construction (Agilent SureSelect Human All Exon kit), quality assessment ...
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bioRxiv - Genomics 2019Quote: ... SureSelectXT Human all exon V6 kit (Agilent Technologies) was used to capture exons ...
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bioRxiv - Immunology 2022Quote: ... and mouse anti-human CD68 (Dako, M087601-2) and rabbit anti-human PD-L1 (Ventana ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC for human Ki67 (IR62661-2, Agilent Technologies) was used to ensure the propagated tumors were comprised of human cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Genomics 2019Quote: ... Universal Human Reference RNA was purchased from Agilent Technologies (#740000 ...
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bioRxiv - Biophysics 2019Quote: The human embryonic kidney AD-293 cells (Agilent) were cultured in growth medium that consisted of Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... A SureSelectXT Human All Exon V6 kit (Agilent) was used for exome capture ...
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bioRxiv - Immunology 2022Quote: ... A whole Human Genome Microarray 4X44K (Agilent Technologies) was used for gene expression analysis ...
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bioRxiv - Immunology 2023Quote: ... rabbit polyclonal anti-Human/mouse vWf (Dako #A0082), rat monoclonal anti-mouse/rat FOXP3 (clone FJK-16s ...
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bioRxiv - Immunology 2024Quote: ... or rabbit anti-human C3d polyclonal Ab (Agilent) primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... libraries were prepared using SureSelect XT Human (Agilent) and captured using a customized panel covering exonic regions of 435 genes ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Cancer Biology 2019Quote: Microarray experiments were performed as dual-color or single-color hybridizations on either Agilent Whole Human Genome 4×44K microarrays (Design ID 014850) or 8×60K human custom (Agilent-048908) microarrays comprising identical features for coding genes ...
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bioRxiv - Genomics 2022Quote: ... Each pool was enriched twice with the SureSelect Human All Exon v7 probes and the latest version of the probes SureSelect Human All Exon v8 (Agilent Technologies) for the second time ...
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bioRxiv - Molecular Biology 2023Quote: ... The reporter cell lines were stained with anti-human CD45 and anti-human CD19 antibodies and analyzed by flow cytometry on an ACEA NovoCyte 2060 (Agilent, USA). ACEA NovoExpress (Agilent ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...