Labshake search
Citations for Agilent :
1 - 50 of 6783 citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... JNK3 kinase dead (K55R mutant) cDNA was generated by Quickchange (Stratagene). mCherry-tagged JIP3 in FEW vector was as previously described (Holland et al ...
-
bioRxiv - Biochemistry 2019Quote: ... Pyk2 FERM-kinase mutants were generated using the QuikChange (Agilent Genomics) site-directed mutagenesis strategy ...
-
bioRxiv - Cancer Biology 2019Quote: Next generation sequencing of 515 kinase genes was performed by Agilent Kinome capture and run on the Illumina MiSeq at PE150bp ...
-
bioRxiv - Cancer Biology 2024Quote: ... TSSK6 kinase mutations were made using the QuikChange XL Site-Directed Mutagenesis Kit (200516, Agilent). pLX302-TSSK6-V5 was used as a template ...
-
bioRxiv - Molecular Biology 2022Quote: ... Generation of the kinase inactive variants was achieved using QuikChange PCR (Agilent).
-
bioRxiv - Biochemistry 2023Quote: ... and Y402F Pyk2 FERM−kinase mutants were generated using the QuikChange (Agilent Genomics) site-directed mutagenesis strategy ...
-
bioRxiv - Biochemistry 2022Quote: ... Pyk2 FERM– kinase mutants in pNAM004 were generated using the QuikChange (Agilent Genomics) site-directed mutagenesis strategy ...
-
bioRxiv - Cell Biology 2022Quote: ... K22M mutation of the kinase dead PMVK mutant (PMVKmut) was generated using QuickChange Site-Directed Mutagenesis Kit (Agilent, Catalog # 200518). Lentiviral particles were used to constitutively express shPMVK (pLV[shRNA]-Hygro-U6> hPMVK[shRNA#4] ...
-
bioRxiv - Cancer Biology 2020Quote: ... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
-
bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
-
bioRxiv - Genetics 2021Quote: ... as well as a kinase-dead control (K396H) 16,were generated by single-site mutagenesis (Agilent 210518) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Each of the 430 compounds from a SelleckChem Kinase Inhibitor Library (catalog #L1200) were combined at 100 μM in wells of a polypropylene 96-Well Tube Plates (Agilent) with TcPINK1 KD at 0.5 mg/mL (approximately 10 μM ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The activated trimer was purified by HPLC (Agilent) over an Eclipse XDB C18 column (Agilent ...
-
bioRxiv - Cancer Biology 2021Quote: ... Rabbit-Mouse kit (Dako, Denmark). Sections were counterstained with haematoxylin (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rabbit-Mouse kit (Dako, DK). Sections were counterstained with haematoxylin (Sigma-Aldrich Inc.) ...
-
LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: Seahorse experiments were performed on sorted CD8+ cells activated in presence or absence of LSDi using XF Cell Mito Stress kit (Seahorse Bioscience). OCR and ECAR were measured with XF96 Extracellular Flux Analyzers (Seahorse Bioscience) ...
-
bioRxiv - Neuroscience 2021Quote: ... and activated astrocytes (GFAP; Dako; Santa Clara, CA; Catalogue No Z0334). Moreover ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
-
bioRxiv - Physiology 2021Quote: ... Plasma insulin was determined with ELISA (Dako Denmark) according to manufacturer’s instructions.
-
bioRxiv - Biochemistry 2021Quote: ... A secondary quantification measurement with Bioanalyzer Protein Analysis Kits (Agilent) provided similar concentration values as SYPRO Red assay (data not shown).
-
bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualised using DAB (Dako Real Envision kit) for 3-5 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... by adding prior heat-activated 200 units Pfu Turbo Cx (Agilent Technologies, Inc., 600414) in 50 μL of 1X PfuCx buffer at 72°C for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... and SureSelectXT Mouse Methyl-Seq Reagent Kit (Agilent Catalog # 931052) ...
-
bioRxiv - Molecular Biology 2022Quote: ... or Dako EnVision + TM Peroxidase Mouse Kit (DAKO, Glostrup, Denmark) with 3,3’-diaminobenzidine as substrate ...
-
bioRxiv - Genomics 2020Quote: ... As described in manufacturer’s protocol (Agilent SureSelectXT Mouse Methyl-Seq Kit), bisulfite converted libraries were PCR-amplified for 8 cycles with supplied universal primers and purified using AMPure XP beads ...
-
bioRxiv - Neuroscience 2022Quote: ... and the Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent ...
-
bioRxiv - Neuroscience 2024Quote: ... and the Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent ...
-
bioRxiv - Cancer Biology 2024Quote: ... One microgram or more of mouse genomic DNA from each sample was analyzed by whole exome sequencing using the SureSelectXT Mouse All Exon kit (Agilent), followed by next generation sequencing using the NovaSeq 6000 S4 flow cell (Illumina ...
-
bioRxiv - Physiology 2021Quote: ... Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A., Spain) was used for the qualitative identification of antigens by light microscopy ...
-
bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP ...
-
bioRxiv - Cell Biology 2020Quote: ... A gate pulse provided by a pulser (Berkley Nucleonics Corporation, 565) activated a MW generator (Agilent, E8257D) to output a MW pulse ...
-
bioRxiv - Immunology 2022Quote: ... heat-activated antigen retrieval of deparaffinized/dehydrated sections was performed using Target Retrieval Solution (Dako, Glostrup, Denmark). After the blocking of endogenous peroxidases using Peroxidase-Blocking Solution (Dako) ...
-
bioRxiv - Genetics 2023Quote: ... Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE8×60K Microarray kit (design ID:028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... Whole exome sequencing (WES) was performed using the Mouse All Exon kit (Agilent) for target capture followed by next-generation sequencing by Psomagen ...
-
bioRxiv - Microbiology 2021Quote: ... After deparaffinization, antigens were activated (121°C, 10 min) with Target Retrieval Solution pH6.0 (Dako Cytomation, Glostrup, Denmark), and endogenous HRP was inactivated by hydroperoxide treatment ...
-
bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
-
bioRxiv - Microbiology 2020Quote: ... A series of alanine mutants were introduced into the SARS-CoV-2 spike protein using the QuickChange mutagenesis kit or the QuickChange multi-mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
-
bioRxiv - Immunology 2023Quote: ... The plate was then measured using a BioTek ELX808 ELISA reader (Agilent) at 450nm and 620-650nm ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
-
bioRxiv - Cancer Biology 2021Quote: ... rabbit/mouse following manufacturer’s instructions (Detection Kit #K5005, Agilent Technologies, Santa Clara, CA, USA). Immunostained tissue sections were counterstained with hematoxylin solution according to Mayer (T865.1 ...
-
bioRxiv - Immunology 2020Quote: Whole-exome sequencing libraries were constructed using the SureSelect Mouse all Exon Kit (Agilent). RNA sequencing libraries were constructed using the TrueSeq Stranded mRNA Sample Prep kit (Illumina) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated in HRP rabbit/mouse secondary antibody (DAKO Real EnVision kit) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: RNA was extracted from mouse lungs using the Absolutely Total RNA Purification Kit (Agilent). RNA extraction from cell culture experiments were performed using the Qiagen RNeasy kit (Qiagen) ...
-
bioRxiv - Immunology 2021Quote: ... and Protein block (Protein block (Dako) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
-
bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
-
bioRxiv - Neuroscience 2022Quote: ... Libraries were prepared according to Illumina’s instructions and SureSelectXT Mouse All Exon enrichment kits (Agilent) were used ...
-
bioRxiv - Cell Biology 2019Quote: RNA was extracted from mouse embryonic stem cells using the Absolutely RNA microprep kit (Agilent). 1 µg of RNA was pre-incubated with 4.5 µg of (PR)20 or water for 10 min at room temperature ...