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Citations for Agilent :
1 - 50 of 6811 citations for Mouse Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HA-RAMP3ΔPDZ and SNAP-RAMP3ΔPDZ constructs were generated by removing the DTLL PDZ recognition sequence through site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis and SNAP-RAMP and Nluc-GPCR generation was confirmed by Sanger sequencing (Department of Biochemistry ...
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bioRxiv - Biochemistry 2019Quote: ... The CFTR PDZ-binding motif (CFTR residues 1477-1480) was inserted downstream using Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... and programmed cell death ligand 1 (PD-L1; clone 22C3, pharmDx kit, Agilent, USA) were used ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding the ACR-16 TM3-4 loop or the SDN-1 intracellular domain full length or lacking the EYFA PDZ binding motif were cloned to pGEX-3X expression vector and then transfected into Arctic express E.coli strain (Agilent). Bacterial clones were first cultured overnight in 5 mL LB medium supplemented with 100 mg/mL ampicillin ...
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bioRxiv - Neuroscience 2023Quote: ... neuronal media was changed to XF DMEM (Agilent) containing 1mM pyruvate ...
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bioRxiv - Cell Biology 2020Quote: Citrate Synthase Normalization Assay was performed on Seahorse XF96 Cell Culture Microplate (Agilent) right after seahorse assay or on plates frozen at −80°C with leftover medium aspirated ...
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bioRxiv - Immunology 2021Quote: ... cells were sequentially injected with 1.5μM oligomycin A (ATP synthase inhibitor; Agilent Technologies), 0.5μM FCCP (mitochondrial uncoupling agent ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Biophysics 2022Quote: ... ligand content was analyzed using an HPLC system (Agilent 1100 Series) and samples were separated using a Zorbax Eclipse XDB-C18 column (Methanol 70% isocratic condition ...
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bioRxiv - Neuroscience 2023Quote: ... neuronal cells were plated in a XF96 cell culture microplate (Agilent Technologies) at a density of 70,000 cells/well ...
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bioRxiv - Bioengineering 2023Quote: ... using a 1.5% w/v alginate solution in deuterium oxide (64 scans; Agilent 400 MHz Premium COMPACT equipped with Agilent OneNMR Probe ...
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bioRxiv - Cancer Biology 2021Quote: ... Rabbit-Mouse kit (Dako, Denmark). Sections were counterstained with haematoxylin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Rabbit-Mouse kit (Dako, DK). Sections were counterstained with haematoxylin (Sigma-Aldrich Inc.) ...
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Cell Biology 2021Quote: ... The N-terminal region was removed by a Quikchange reaction (Agilent), and the resulting shortened fragment was inserted into the pDEST-HisMBP destination vector via a Gateway reaction.
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in mito stress test media with or without ligand(s) (XF base media (Seahorse Bioscience) supplemented with 1mM sodium pyruvate (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin was determined with ELISA (Dako Denmark) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Biophysics 2022Quote: TRPV4 N-terminal constructs were expressed in Escherichia coli BL21-Gold(DE3) (Agilent Technologies) grown in terrific broth (TB ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... A secondary quantification measurement with Bioanalyzer Protein Analysis Kits (Agilent) provided similar concentration values as SYPRO Red assay (data not shown).
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualised using DAB (Dako Real Envision kit) for 3-5 minutes ...
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bioRxiv - Neuroscience 2023Quote: Neuronal mitochondrial function following vehicle or cytokine treatment was characterized on the Seahorse XFe24 Extracellular Flux Analyzer platform (Agilent). The Seahorse Analyzer measures oxygen consumption rate (OCR ...
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bioRxiv - Cell Biology 2022Quote: ... and SureSelectXT Mouse Methyl-Seq Reagent Kit (Agilent Catalog # 931052) ...
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bioRxiv - Molecular Biology 2022Quote: ... or Dako EnVision + TM Peroxidase Mouse Kit (DAKO, Glostrup, Denmark) with 3,3’-diaminobenzidine as substrate ...
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bioRxiv - Bioengineering 2023Quote: ... using a 1.5% w/v alginate solution in deuterium oxide (64 scans; Agilent 400 MHz Premium COMPACT equipped with Agilent OneNMR Probe) and analyzed using MestrNova Software (14.6 ...
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bioRxiv - Genomics 2020Quote: ... As described in manufacturer’s protocol (Agilent SureSelectXT Mouse Methyl-Seq Kit), bisulfite converted libraries were PCR-amplified for 8 cycles with supplied universal primers and purified using AMPure XP beads ...
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bioRxiv - Neuroscience 2022Quote: ... and the Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... and the Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Cancer Biology 2024Quote: ... One microgram or more of mouse genomic DNA from each sample was analyzed by whole exome sequencing using the SureSelectXT Mouse All Exon kit (Agilent), followed by next generation sequencing using the NovaSeq 6000 S4 flow cell (Illumina ...
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bioRxiv - Physiology 2021Quote: ... Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A., Spain) was used for the qualitative identification of antigens by light microscopy ...
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bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... PARLSkd3 and variants were expressed with an N-terminal MBP-tag in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in lysis buffer (40 mM HEPES-KOH pH = 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... which is mimicking serotypes AAV8 and AAV9 with the inverted terminal repeats (ITRs) from AAV2 (Agilent Technologies) that contains the CMV promoter and subsequently purified according to published protocol (47) ...
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bioRxiv - Genetics 2023Quote: ... Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE8×60K Microarray kit (design ID:028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Cell Biology 2023Quote: ... Whole exome sequencing (WES) was performed using the Mouse All Exon kit (Agilent) for target capture followed by next-generation sequencing by Psomagen ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...