Labshake search
Citations for Agilent :
701 - 750 of 1488 citations for Mouse BNIP2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... and the SureSelectXT Mouse Methyl-Seq target enrichment panel (Agilent, #5191-6704) following the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2024Quote: ... The primary antibody was mouse anti-human Aβ (Dako #M0872; 1:400) and the secondary antibody was Vectastain ABC kit anti-mouse secondary antibody (Vector Laboratories #PK-4002 ...
-
bioRxiv - Neuroscience 2024Quote: ... or mouse anti-NeuN (Chemicon, MAB337) and anti-GFAP (Dako, GA52461-2) antibodies ...
-
bioRxiv - Genetics 2021Quote: ... cloned into an expression plasmid driven by CMV promoter and modified using QuikChange Site-Directed Mutagenesis Kit (Agilent). For each affinity purification ...
-
bioRxiv - Immunology 2019Quote: Mutations were introduced into plasmid constructs using the QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies, San Jose, CA) as recommended by the manufacturer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 34 plasmids encoding new quantification tag (LVXXLTK) were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies) using appropriate DNA primers (Table S7) ...
-
bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
-
bioRxiv - Microbiology 2020Quote: ... S27 isolate-based plasmid via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). The following mutations were introduced ...
-
bioRxiv - Microbiology 2021Quote: ... plasmid was reacted with primer pairs designed to introduce the desired mutations using Quikchange kit (Agilent, cat# 600670). After digestion with the restriction enzyme DpnI (NEB ...
-
bioRxiv - Microbiology 2022Quote: ... IN mutations were introduced in the pLR2P-VprIN plasmid using the QuikChange Site- Directed Mutagenesis kit (Agilent Technologies). The presence of the desired mutations and the absence of unwanted secondary changes was assessed by Sanger sequencing.
-
bioRxiv - Microbiology 2019Quote: ... IN mutations were introduced into the pLR2P-vprIN plasmid using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies). Presence of the desired mutations and absence of unwanted secondary changes were verified by Sanger sequencing.
-
bioRxiv - Immunology 2019Quote: ... R148Q mutation was generated by site-directed mutagenesis on a WT STAT2 plasmid using QuickChange II PCR (Agilent). Lentiviral particles were generated by co-transfection of STAT2 ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were then transformed into XL-10 Gold chemically competent Escherichia coli cells (Agilent Technologies, Santa Carla, CA) as previously described [14] ...
-
bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis of these plasmids for C483A or Pol(-) mutants were obtained with the QuikChange kit (Stratagene) using primers listed in Supplementary Table 1 ...
-
bioRxiv - Cell Biology 2019Quote: ... the pMTT1-GFP-LF4A plasmid was subjected to site-directed mutagenesis using the QuikChange Lightning kit (Agilent 210518) with the primers 5’-CAGGACGTTTGGCACTAGTGGCTGAATTGATGGATCAGAACC-3’ and 5’-GGTTCTGATCCATCAATTCAGCCACTAGTGCCAAACGTCCTG-3’.
-
bioRxiv - Microbiology 2020Quote: ... The plasmid for production of (His)6-SUMO-σAntA-DD was generated by site-directed mutagenesis (Agilent QuikChange) using primers listed in Table S3 ...
-
bioRxiv - Microbiology 2021Quote: ... Mutations were introduced to the M plasmid using the QuikChange Lightning site-directed mutagenesis (Agilent, Santa Clara, CA) protocol ...
-
bioRxiv - Cell Biology 2019Quote: ... Introducing stop codon at residue 561 in pCS2-Hs BMP2K plasmid using QuikChange site-directed mutagenesis (Agilent Technologies) yielded pCS2-Hs BMP2KΔCT ...
-
bioRxiv - Immunology 2021Quote: A pAAV-MCS plasmid containing inverted terminal repeats (ITRs) from AAV serotype 2 (Agilent Technologies, Santa Clara, CA) was utilized as backbone for AAV6 plasmid construction (naturally occurring AAV6 has an AAV2 ITR (24)) ...
-
bioRxiv - Biophysics 2022Quote: ... A plasmid to express the T238V mutation of Tub2p (yeast β-tubulin) was made by QuikChange mutagenesis (Stratagene), using an expression plasmid for wild-type Tub2 as template and with primers designed according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: A plasmid encoding 6xHis-tagged methionyl-tRNA synthetase (MetRS) was transformed into BL21 (DE3) Codon+ RIL cells (Agilent). Overnight cultures were diluted (1:100 ...
-
bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: ... was used as a template for generating expression plasmids with FH variants using the QuikChange II kit (Agilent). Sanger sequencing was used to confirm sequence identity for all plasmids ...
-
bioRxiv - Biophysics 2024Quote: The WT-CtNAT10-pFastBacHT A plasmid was used to prepare all CtNAT10 mutants using the QuikChange protocol (Stratagene) 53 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Expression plasmids were constructed with the obtained PCR product using the SureVector system (Agilent, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... All GαS variants were obtained from this plasmid by site-directed mutagenesis using the QuikChange II kit (Agilent) and primers listed in Table S2 ...
-
bioRxiv - Genomics 2024Quote: ... the pETM11-Sumo3-Tn5(E54K,L372P) plasmid was transformed into Escherichia coli BL21(DE3) codon + RIL cells (Stratagene). Cells were grown in TB-FB media (1.2 % Bacto-Tryptone ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN anti-mouse EMD Millipore MAB377, GFAP anti-mouse, PSD-95 anti-rabbit abcam ab18258, S100B anti-rabbit Dako Z0311, SYT-1 anti-goat LSBio LS-B5899), secondary antibodies (488 anti-mouse ...
-
bioRxiv - Genetics 2021Quote: ... we introduced the corresponding single nucleotide mutation in the 3xFLAG donor plasmid using QuikChange Site-Directed Mutagenesis Kit (Agilent). Then this donor plasmid was used for the same process used to generate the BAG3WT-FLAG line25 ...
-
bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmid point mutations were generated using Pfu Ultra polymerase by Quick Change PCR mutagenesis (Agilent Technologies, Santa Clara, CA). The resulting DNA constructs and strains were verified by DNA sequencing.
-
bioRxiv - Microbiology 2020Quote: ... Unit genome-length fragments (approximately 2,600 nucleotides) were excised and ligated into the pBLUESCRIPT-KS+ (pKS+) plasmid vector (Stratagene), previously cleaved with the same enzyme ...
-
bioRxiv - Cell Biology 2021Quote: ... A plasmid with smc3-K112,113R (AMp1392) was generated by site-directed mutagenesis (using QuikChange XL Site-directed mutagenesis kit, Agilent). Both plasmids were integrated into the LEU2 locus in the pCLB2-3HA-SMC3 strain background.
-
bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).
-
bioRxiv - Microbiology 2022Quote: Single-base alterations to plasmid sequences were performed using a PCR technique based on QuikChange site-directed mutagenesis (Stratagene).
-
bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis of the human yb-1 coding gene was carried out directly on the pET22b-YB-1_1-180 expression plasmid by using the “Quikchange II XL site-directed mutagenesis kit” from Stratagene and appropriate oligonucleotides (Eurofins Genomics) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we deleted two existing Bsa-I sites from the plasmid by site-directed mutagenesis (Agilent, Santa Clara, CA, #200521). In the clonal strain production ...
-
bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
-
bioRxiv - Microbiology 2021Quote: ... Storz and pRprAmut2 was constructed by amplification of the pRprA plasmid with mutagenic primers using the Pfu enzyme (Agilent), followed by DpnI digestion and transformation into the cloning strain NEB5-alpha F’Iq ...
-
bioRxiv - Microbiology 2019Quote: ... and pGal4-UAS-Luc plasmids (0.3 µg/well; provided by Dr. Yves Jacob) or IFN-β-pGL3 (0.3 µg/well; Stratagene) together with pRL-CMV reference plasmid (0.03 µg/well ...
-
bioRxiv - Immunology 2019Quote: ... the parental Env-encoding plasmid was altered by site-directed mutagenesis using the QuikChange site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2021Quote: ... The region containing random fragments of the VWF plasmid was made double stranded using primers P9/P10 (annealing temperature = 65.0°C) and Herculase II (Agilent) with 16-20 rounds of PCR amplification (Figure S1B) ...
-
bioRxiv - Genomics 2021Quote: ... coli were generated by introducing the corresponding mutations in plasmid pDL893 using the QuikChange site-directed mutagenesis kit (Stratagene).
-
bioRxiv - Biochemistry 2021Quote: Individual point mutations were introduced into AsCap5 plasmid by Quick-change mutagenesis according to the direction provided by Agilent. Oligonucleotides employed for creating mutants were ordered from IDT ...
-
bioRxiv - Neuroscience 2021Quote: ... Fusion products were digested with NotI and ligated to a NotI cleaved pAAV-MCS plasmid backbone (#240071-5, Stratagene). The final rAAV/SORL1 KO-Neo plasmid construct was verified by NotI digestions and sequencing ...
-
bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
-
bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent), 0.2 μM oligonucleotide encoding the change ...
-
bioRxiv - Microbiology 2022Quote: ... the vector pEC1_ptpA previously constructed [22] was used as template to generate the suicide plasmid pEC1_ptpA_D120A derivative by using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) with the primer #1559 (5’-GGAAGAGAGTGATGTACCAGCTCCATACTACACGAATAATT-3’) ...
-
bioRxiv - Cancer Biology 2022Quote: ... PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following the manufacturer’s instructions (QuickChange II, Stratagene). shPLK1 UTR ...