Labshake search
Citations for Agilent :
1 - 50 of 2603 citations for L Serine 1 13C 99%; 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Incorporation of 13C was quantitated and corrected for natural 13C abundance using Profinder B.08.00 (Agilent Technologies). LC-MS data was deposited in the MetaboLights database47 under the accession code MTBLS2374 (www.ebi.ac.uk/metabolights/MTBLS2374).
-
bioRxiv - Genomics 2020Quote: ... 15N-labeled metabolite analysis was performed using LC-TOF G6230A (Agilent Technologies) instrument equipped with 1290 Infinity HPLC system ...
-
bioRxiv - Biochemistry 2020Quote: ... 1D 15N T1/T2 relaxation spectra were processed and analyzed with VnmrJ v4.2 (Agilent Inc). Macro “tc” (wiki.nesg.org ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
-
bioRxiv - Synthetic Biology 2023Quote: ... NMR spectroscopic data (1H, 13C, HSQC, COSY, and HMBC) were collected by Agilent 600 MHz (14.1 Tesla ...
-
bioRxiv - Genetics 2022Quote: ... and antimycin/rotenone (1 mmol/L and 1 mmol/L) (Cell Mito Stress Test Kit, Agilent, #103015100). Analysis was carried out by using the Seahorse analyzer software.
-
bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... All 13C NMR spectra were obtained with a Varian VNMRS 600 MHz NMR (Agilent) spectrometer equipped with a 3 mm standard probe ...
-
bioRxiv - Plant Biology 2022Quote: ... Compounds were then further purified to ≥98% purity by HPLC (Agilent 1100 Series) equipped with a diode array UV detector ...
-
bioRxiv - Biochemistry 2021Quote: ... and a serine at position 61 was replaced by a cysteine using the Quikchange kit (Agilent). GST-PHPLCδ1 was expressed in E ...
-
bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
-
bioRxiv - Physiology 2022Quote: ... [U-13C]-palmitate enrichment and [1,1,2,3,3-2H]-glycerol enrichment were measured by GC-MS/MS (Agilent GC model 7890A and Waters Quattro Micro) ...
-
bioRxiv - Plant Biology 2020Quote: ... Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent) using primers detailed in Supplemental Table 2 ...
-
bioRxiv - Biochemistry 2023Quote: ... CLEANEX and DOSY data were collected on 100 μM 15N-labelled protein at a proton Larmour frequency of 600 (Agilent) or 800 (Bruker ...
-
bioRxiv - Biochemistry 2023Quote: ... B: 98% acetonitrile/0.1% FA in water) (1290 Infinity II LC system, Agilent Technologies, Waldbronn, Germany). The temperature within the customized LC system was kept at 0 °C to minimize the back exchange ...
-
bioRxiv - Genetics 2019Quote: ... Serine-to-alanine mutation was generated using QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, CA, USA 200522) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... we substituted in the VH domain the serine at position 120 to an unpaired cysteine (14.4.4 scFV) via site directed mutagenesis (QuikChange II, Agilent technologies). In addition ...
-
bioRxiv - Cell Biology 2024Quote: ... The cysteine codons 233 and 235 of NBR9 were mutagenized to serine codons by QuikChange mutagenesis (Agilent, Waldbronn, Germany) to give plasmid pRK1891.
-
bioRxiv - Cancer Biology 2024Quote: ... The CDKN1B Serine 10 to alanine mutant reporter was cloned using the Quikchange II site-directed mutagenesis kit (Agilent).
-
bioRxiv - Cancer Biology 2022Quote: ... The pCS2HA-HA-LIX1 plasmid was used as template to generate the LIX1 variants in which cysteine 83 and 84 were substituted by serine residues with the QuikChange Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s protocol and the primers listed in supplemental Table S1.
-
bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
-
bioRxiv - Molecular Biology 2022Quote: ... AAV-LK03insT and AAV-AM were created by inserting a Threonine or Glycine respectively immediately downstream of the Serine at position 264 of the AAV-LK03 capsid using the QuikChange in vitro mutagenesis kit (Agilent).
-
bioRxiv - Microbiology 2023Quote: ... The produced 13C-CO2 was measured directly from the exetainer headspace with a GC-MS (Agilent 5975C inert MSD). Liquid 13CO2 concentrations were calculated with the Henry coefficient (Supplementary Infomration) ...
-
bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
-
bioRxiv - Microbiology 2020Quote: ... a non-conserved cysteine (C232) was mutated to a serine with the aid of the QuickChange Lightning Site Directed Mutagenesis kit (Agilent technologies) and confirmed with DNA sequencing (ACGT DNA Technologies Corporation) ...
-
bioRxiv - Biophysics 2020Quote: ... The VDAC1 mutations at serine 215 to glutamate (S215E) was generated by PCR with QuikChange site-directed mutagenesis kit (Agilent Technology) using pET-VDAC1 as cDNA templates and the following primers ...
-
bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
-
bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
-
bioRxiv - Microbiology 2022Quote: ... the increase of produced 13C-CO2 was measured directly from the headspace with a GC-MS (Agilent 5975C inert MSD). In addition ...
-
bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
-
bioRxiv - Cancer Biology 2019Quote: ... For antigen retrieval the slides were incubated for 30 min at 98 °C in 1x Target retrieval solution (Dako). Sections were stained by adding primary antibodies diluted in immunofluorescence buffer (IFB ...
-
bioRxiv - Microbiology 2020Quote: ... sections underwent antigen retrieval in citrate buffer (pH 6.0, 20 min at 98°C) followed by blocking of endogenous peroxidase (peroxidase block, S2023, Dako) for 10 min at room temperature (RT) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using Dako Citrate buffer (pH 6.0) for 30 minutes at 98°C in a PT Link rinse station (Dako). Tissue sections were blocked in PBTx containing 5% BSA (Merck ...
-
bioRxiv - Neuroscience 2021Quote: ... + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat. no. Z0334), or mouse anti-GFAP (1:500 dilution ...
-
bioRxiv - Cancer Biology 2023Quote: ... 70 kDa subunit (NF-L, 1:500, Agilent, Santa Clara, CA, #M0762). Immunostained sections were counterstained with hemalum.
-
bioRxiv - Immunology 2019Quote: ... as internal standard were analyzed by 1D 1H and 1H{13C}-HSQC NMR on a 14.1 T DD2 NMR spectrometer (Agilent Technologies, CA). 1D 1H spectra were acquired using the standard PRESAT pulse sequence with 512 transients ...
-
bioRxiv - Immunology 2020Quote: ... succinate and malate and their corresponding 13C labeled counterparts were extracted and deconvoluted using MassHunter software (Agilent Technologies, New Castle, DE). Retention time consistency was manually rechecked and compared to authentic compounds that were injected under similar chromatographic conditions ...
-
bioRxiv - Molecular Biology 2020Quote: All 2D heteronuclear 15N-1H NMR experiments were acquired on a 11.75 Tesla Varian 500 MHz VNMRs system (Agilent Technologies Inc., Palo Alto, CA) with an operational frequency of 499.84 MHz ...
-
bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
-
bioRxiv - Neuroscience 2023Quote: ... Deparaffinization and antigen retrieval was performed in the low pH EnVision FLEX Target Retrieval Solution at 98 °C for 24 minutes in the DAKO PT link from Agilent. Antibodies were diluted in DaVinci Green Diluent (Biocare ...
-
bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peptides were re-dissolved in 100 µL solvent A (0.1% TFA in water/ACN (98:2, v/v)) and injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
-
bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
-
bioRxiv - Immunology 2020Quote: ... Generated data were normalized to viable cell number in million/ml at the viability was found to be consistently between 96% and 99% as determined by trypan blue exclusion assay (ViCell, Beckmann Coulter) and analyzed using Seahorse Glycolysis Stress Test Generator (Agilent).
-
bioRxiv - Neuroscience 2024Quote: ... Nuclear magnetic resonance (NMR) spectra including the 1H and 13C NMR spectra were recorded on a Varian Mercury Plus 400 NMR spectrometer (Agilent Technologies, Santa Clara, CA). Proton chemical shifts are reported as parts per million (δ ppm ...