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Citations for Agilent :
51 - 100 of 110 citations for L Isoleucine 13C6 99%; 15N 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After 16 h of stress in low glucose growth medium (1 g/L; Agilent Technologies, 103577), cells were washed once with PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and G73A Sco-CHH-L) was performed using the QuikChange PCR-mediated Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer's instruction with the plasmid pET-22b(+)-Sco-CHH-L encoding the wild-type Sco-CHH-L (24 ...
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bioRxiv - Plant Biology 2019Quote: ... 20 µl of sample injected and separated on a Zorbax SB-C18 column (1.8-µm, 2.1×1.8mm; Agilent) connected to a Zorbax SB-C18 analytical guard column (5-µm ...
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bioRxiv - Microbiology 2022Quote: ... External calibration was performed prior to data collection using ESI-L Low Concentration Tuning Mix (Agilent Technologies), with hexakis(1H,1H,2H-perfluoroethoxy)phosphazene employed as internal lock-mass calibrant throughout the run ...
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bioRxiv - Neuroscience 2022Quote: N2A cells were plated (12.500 cells/well) on poly-l-lysine-coated XF24 wells plates (Seahorse Bioscience) in DMEM (high glucose ...
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bioRxiv - Microbiology 2023Quote: ... . All the acquired mass spectra were internally calibrated by injecting ESI-L Low Concentration Tuning Mix (Agilent Technologies Netherlands BV ...
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bioRxiv - Systems Biology 2024Quote: ... the MS was calibrated using the ESI-L Low Concentration Tuning Mix (Agilent, Santa Clara, CA, USA). The ESI-L Low Concentration Tuning Mix (diluted 1:4 [v/v] with 75% ACN ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent ...
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bioRxiv - Physiology 2022Quote: ... were placed in 50 µl XF medium (non-buffered DMEM containing 10 mM glucose and 2 mM GlutaMax, pH 7.3-7.4, Agilent), centrifuged in a carrier tray (300g for 3 min with no break) ...
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bioRxiv - Cell Biology 2020Quote: ... pNG72-GFP-L-CHMP4C S210A was generated by site-directed mutagenesis using a commercial kit (Quickchange Lightning, Agilent Technologies). For retroviral production ...
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bioRxiv - Physiology 2019Quote: ... Sections were immersed in EDTA-NaOH buffer (0.37 g/L EDTA, pH 9.0, preheated to 65°C) in Dako PT LINK (Dako; #PT101) and heated to 97°C for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: Calibration of the MS was done by injecting ESI-L Low Concentration Tuning Mix (Agilent, Santa Clara, CA, USA) prior to the measurements ...
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bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Immunology 2019Quote: ... qPCR for the RSV L gene was performed on a Stratagene Mx 3005p (Agilent technologies, Santa Clara, CA, USA) using the following primers:
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bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... the cell culture of MEFs was started (1.6×104 cells/200 micro-l/well (Agilent, XF96 microplate V3-PS)) using a MEF medium ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Immunology 2020Quote: 100μL of PRAK-03202 sample was injected onto Agilent Bio SEC-5 (5μ, 2000Å, 7.8 mm ID × 300mm) (Agilent Technologies) column for analytical SEC analysis and analysed with Agilent Technologies HPLC instrument (1260 Infinity II) ...
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bioRxiv - Biochemistry 2022Quote: ... and meprin α R372T (100 μl at 0.44 mg ml−1) were applied onto an AdvanceBio SEC column (300 Å, 7.8×300 mm, Agilent Technologies). The data was evaluated using ASTRA® 6 (Wyatt Technology).
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of extracted RNAs was loaded on a Agilent Pico 6000 chip and ran on the 2100 Bioanlayzer system (Agilent).
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... In case of ELF1α amplification was carried out in a 15 μL reaction volume containing 7.5 μL of 2X Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix (Stratagene), 0.75 μL of each forward and reverse primers (0.5 μM) ...
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bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
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bioRxiv - Immunology 2022Quote: Sorted CD8 T cells were seeded into poly-l-lysine pre-coated wells of a 96-well plate (XFe96 cell culture microplates, Agilent) and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse ...
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bioRxiv - Molecular Biology 2022Quote: ... at 200 µl min-1 and the digested peptides were captured on a 2 mm x 1 cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... cuticular hydrocarbon extracts were evaporated to a volume of approximately 100 μL and 1 μL was analyzed by using a 6890 gas chromatograph (GC) coupled to a 5975 mass selective detector (MS) by Agilent Technologies (Waldbronn ...
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bioRxiv - Systems Biology 2023Quote: ... 50μL of 200mM ammonium formate pH10 were added to samples and “Fractionation V2.0” was ran on AssayMap BRAVO (Agilent T). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... and ion mobility calibration was performed using ESI-L low concentration tune mix bought from Agilent (Santa Clara, CA, USA). The 10 mM sodium formate solution contained 1 M NaOH (250 μL ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent, Dako, Glostrup, Denmark) were then added to the slides ...
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bioRxiv - Synthetic Biology 2021Quote: ... The mass analyzer was calibrated between m/z 118 and 2721 using an Agilent ESI-L low concentration tuning mix solution (Agilent, USA) at a resolution of 20 000 giving a mass accuracy below 2 ppm ...
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The circadian clock gene circuit controls protein and phosphoprotein rhythms in Arabidopsis thalianabioRxiv - Systems Biology 2021Quote: ... 8 μl were run on an on-line capillary-HPLC-MSMS system consisting of a micropump (1200 binary HPLC system, Agilent, UK) coupled to a hybrid LTQ-Orbitrap XL instrument (Thermo-Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed 2 times with 200 μL of extracellular flux assay medium (DMEM with 25 mM glucose, 2 mM sodium pyruvate, and 2 mM L-glutamine for mitochondrial stress test (Agilent Technologies). Assay medium was then added to each well to make the final well volume 180 uL ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Genomics 2020Quote: ... The DNA was re-suspended in 100 μl of ultrapure water and the quantity and quality determined using a NanoDrop spectrophotometer and a TapeStation 2200 fluorometer (Agilent Technologies).
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bioRxiv - Microbiology 2021Quote: ... the fractions were injected on HPLC for purification performed on an Eclipse+ C18 column (L = 150 mm, D = 3.0 mm, Particles diameter 5 µm) (Agilent, Waldbronn, Germany). The volume injected was 100 µl ...
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bioRxiv - Immunology 2022Quote: ... OCR and ECAR were measured at 37°C in Seahorse XF DMEM medium (pH7.4, with 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate) (Agilent, 103680-100). 1.5 μM Oligomycin ...
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bioRxiv - Synthetic Biology 2023Quote: ... Ten μL was loaded onto a SHODEX ORPak CDBS-453 column and separated chromatographically using a 1290 Infinity II HPLC (Agilent Technologies) and the following gradient ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of RNA samples was used for reverse transcription with the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent; 200820) using random hexamer primers ...
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Cancer Biology 2024Quote: ... A 500-μL aliquot of the digestate was mixed with an equal volume of internal standard (10 μg/L Iridium) and analyzed using ICPMS (Agilent 7700) with an external calibration curve.
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bioRxiv - Systems Biology 2020Quote: ... The instrument was externally calibrated two times per day to 1.0 ppm mass accuracy using ESI-L Low Concentration Tuning Mix (Agilent Technologies, Waldbronn, Germany). Hexakis (m/z 622.029509 ...
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bioRxiv - Immunology 2020Quote: ... sterile XF assay buffer (Seahorse XF RPMI Medium supplemented with 10mM glucose, 2mM L-glutamine, and 1mM pyruvate, pH 7.4; Agilent Technologies, Waldbronn, Germany) was used for experiments ...
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bioRxiv - Plant Biology 2022Quote: ... was injected in a spitless injection mode via multimode autosampler and compounds were separated on a DB5 column (30 m l× 0.32 mm ID× 0.25 μm film thickness) (Agilent J&W Scientific, India). Inlet temperature was constant at 250 °C and carrier helium gas flow was 2 ml min-1 ...
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bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
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bioRxiv - Cancer Biology 2023Quote: ... the system was calibrated via the ESI source with the ESI-L low concentration tuning mix (G1969-85000, Agilent Technologies, Waldbronn, Germany). Mass spectra were visualized using SCiLS Lab 2023a Pro (Bruker Daltonics) ...
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bioRxiv - Microbiology 2023Quote: ... Gas samples from the headspaces (250 μl) were collected using a 1 mL Hamilton’s SampleLock™ syringe and manually injected into a Gas Chromatograph (GC) (Agilent 7820A, AgilentTechnologies). H2 and O2 were separated using a packed column (60/80 Molecular Sieve 5A ...
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bioRxiv - Neuroscience 2024Quote: OCR assay: Cells were washed and incubated for 1 hour with 180 μL assay medium [in mmol/L: 5 glucose (Agilent #103577-100), 1 pyruvate (Agilent #103578-100) ...