Labshake search
Citations for Agilent :
1 - 50 of 621 citations for IL 23 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
bioRxiv - Microbiology 2022Quote: ... 23 using an Autostainer Link 48 (Dako). The deparaffinized sections were exposed to EnVision FLEX target retrieval solution high pH (Agilent ...
-
bioRxiv - Microbiology 2020Quote: ... using a DB-23 capillary column (Agilent) as described previously [26].
-
bioRxiv - Molecular Biology 2020Quote: ... for samples huPrP(23-144)*-Aβ and huPrP(23-230)*-Aβ) or thin wall (36 μl, for sample huPrP(23-144)-Aβ*) rotors from Varian (Agilent) or 3.2 mm thick wall (46.7 μl ...
-
bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
-
bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
-
bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
-
bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
-
bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
-
bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
-
bioRxiv - Plant Biology 2019Quote: ... with a flame ionization detector and a DB-23 column (Agilent, USA) with the following protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
-
bioRxiv - Bioengineering 2021Quote: ... Samples were run through a DB-23 column (Agilent Technologies, Santa Clara, CA) using 2 µl injections in a 25:1 split ratio ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The samples were separated by VF-23 ms chromatographic column (Agilent: 30m(length), 0.25mm(inner diameter) ...
-
bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
-
bioRxiv - Plant Biology 2019Quote: ... equipped with a DB-23 column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies). Nonadecanoic methyl ester (C19:0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 23 RIN values were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, Foster City, CA) and only those with values of >8.5 were processed further ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 μL of AccuScript Hi-Fi (Agilent Technologies) was added up to a 10 μL volume ...
-
bioRxiv - Microbiology 2022Quote: ... A 300×7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
-
bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
-
bioRxiv - Systems Biology 2023Quote: ... A 300 × 7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
-
bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
-
bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
-
bioRxiv - Pathology 2023Quote: ... and chromatographic separation using a DB-23 column (60 m, 0.25 mm, 0.15μm, 122-2361 Agilent, US). FAs were identified on the basis of the retention times of analytes and the fragmentation reactions of quasimolecular ions of carboxylic acid ethyl esters and/or their adducts with ammonia [10] ...
-
bioRxiv - Bioengineering 2021Quote: ... using a 7890A GC system fitted with DB-23 columns (30 m x 0.25 mm i.d. x 0.25 µm) (Agilent Technologies). Tripentadecanoin was added to the lipid fractions prior to transmethylation to provide an internal standard for quantification.
-
bioRxiv - Bioengineering 2020Quote: ... using a 7890A GC system fitted with DB-23 columns (30 m × 0.25 mm i.d. × 0.25 μm) (Agilent Technologies). Seed oil and moisture contents of whole seeds were measured by low-resolution time domain NMR spectroscopy using a Minispec MQ20 device (Bruker ...
-
bioRxiv - Microbiology 2020Quote: ... 6890 gas chromatograph fitted with a capillary DB-23 column (30 m x 0.25 mm; 0.25 μm coating thickness; J&W Scientific, Agilent). Helium was used as carrier gas at a flow rate of 1 ml/min ...
-
bioRxiv - Biochemistry 2020Quote: Point mutations in ABCB1 were introduced into 35S:ABCB1-YFP (26) and 35S:ABCB14-GFP (23) using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent).
-
bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...
-
bioRxiv - Neuroscience 2020Quote: The MAPK/ERK pathway was assayed in HEK293 cells with the Elk1 trans-reporting system (PathDetect, Agilent). HEK293 cells were maintained in DMEM supplemented with 10% FBS ...
-
bioRxiv - Neuroscience 2021Quote: AAV1/2 viruses were produced by large-scale triple CaPO4 transfection of HEK293-AAV cells (#240073, Stratagene) as described previously (Van Loo et al. ...
-
Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... AAV9-CAG-tdTomato and AAV-KP1-CAG-tdTomato were produced in HEK293 cells (RRID: CVCL-6871, Agilent) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride density-gradient ultracentrifugation followed by dialysis as described previously.53 AAV9 and AAV-KP1 helper plasmids were provided by J ...
-
bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
-
bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
-
bioRxiv - Plant Biology 2021Quote: ... coupled with an FID detector equipped with a capillary DB-23 column (30 m × 0.25 mm, 0.25 µm coating thickness, Agilent, Waldbronn, Germany) was used ...
-
bioRxiv - Molecular Biology 2020Quote: The conservative lysine at position 23 was replaced with glutamic acid to create Meier-Gorlin mutation (K23E) following site-directed mutagenesis protocol (Agilent). Drosophila-Human and Human-Drosophila hybrids were designed by using a PCR technique ...
-
bioRxiv - Genetics 2020Quote: ... The previously reported RALY intronic duplication 23 was genotyped by size differentiation of PCR products on a Fragment Analyzer (Agilent). Five PCR-assays (ventral promoter assays 1 ...
-
bioRxiv - Genetics 2021Quote: ... size selecting on the SageELF platform (Sage Science) to give a tight fraction of around 23 kbp by FEMTO Pulse analysis (Agilent). The library was sequenced on one SMRT Cell 8M using v2 chemistry ...
-
bioRxiv - Biophysics 2022Quote: ... and we checked that we obtained a DTCCSHe within 785-791 Å2 of its previously determined value (788 Å2).23 The data were extracted using the IM-MS Browser software version B.08.00 (Agilent Technologies).
-
bioRxiv - Microbiology 2022Quote: ... 16S and 23S rRNA ratio and RNA integrity number were calculated from 2100 Expert software and RIN Beta Version Software (Agilent), respectively ...
-
bioRxiv - Plant Biology 2023Quote: ... the associated fatty acids were chemically transmethylated and analyzed by gas chromatography using a GC-2010 plus GC/FID-system (Shimadzu) equipped with a 30 m x 250 µm DB-23 capillary column (Agilent) as previously described (Launhardt et al ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was performed using Accuscript Hi Fidelity First Strand Synthesis kit (Agilent). The amount of RNA entered into the reaction was normalised between samples ...
-
bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
-
bioRxiv - Molecular Biology 2019Quote: pTrex-6×His-TOP1Y723F was generated by QuikChange II XL SDM kit (Agilent) using oligonucleotides 5’-CTAGGGTCCAGAAAATTGAGTTTGGAGGTTCCCAGG-3’ pTrex-6×His-TOP1 K117 ...
-
bioRxiv - Physiology 2021Quote: ... and resuspended in dichloromethane for injection into a 6890N gas chromatograph (Hewlett Packard, Palo Alto, CA, USA) with a J&W Scientific High Resolution Gas Chromatography Column (DB-23, Agilent Technologies) and a flame ionization detector ...
-
bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... Sections of human tumors were stained with mouse anti-human CD68 (Dako) and goat anti-human TSP-4 (AF2390 ...