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Citations for Agilent :
251 - 300 of 8092 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 2 mM glutamine and analyzed with an XF-96 Extracellular Flux Analyzer (Agilent). Four consecutive measurements were obtained under basal conditions followed by the addition of 1 μM oligomycin ...
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bioRxiv - Epidemiology 2019Quote: ... OGTT plasma insulin concentrations were measured by ELISA (Dako UK Ltd., Ely, Cambs, U.K.). Intra-assay imprecision (CV ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: Human ISG15 cDNA was amplified by RT-PCR from universal human reference RNA (Agilent 740000-41), adding BamHI and NotI restriction sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Cell Biology 2023Quote: Non-codon optimised sequences were amplified from a human cDNA library (MegaMan human transcription library, Agilent). Mis18α ...
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bioRxiv - Neuroscience 2020Quote: Total lysate and synaptoneurosomes isolated from cortex tissue of 1-month-old C57Bl/6 mice were UV crosslinked (100 mJ/cm2 for 2 cycles) using UV Stratalinker 2400 (Stratagene) and stored at −80°C until use ...
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bioRxiv - Microbiology 2022Quote: We extracted extracellular metabolites from the seawater matrix using Bond Elut PPL cartridges (1 g/6 ml sized cartridges, Agilent) following the protocol of Dittmar et al ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA-resistant versions of Cofilin-1 have been obtained by mutating 6 bp of the siRNA-targeting sequence using QuickChange (Agilent). Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche) ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Biochemistry 2022Quote: ... 6 - trisulfonic acid (ProZyme, Inc., San Leandro, CA) at the reducing termini by reductive amination ...
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bioRxiv - Microbiology 2023Quote: ... pH 6 (K8005, DAKO, Agilent Technologies Inc., USA). Tissue sections were blocked for endogenous peroxidase and non-specific binding by incubating with 30% H2O2 (H1009 ...
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bioRxiv - Microbiology 2023Quote: ... pH 6 (K8005, DAKO, Agilent Technologies Inc., USA). Tissue sections were blocked for endogenous peroxidase and non-specific binding by incubating with 30% H2O2 (H1009 ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako, M0823, 1:100) and Alexa488-conjugated goat anti-mouse secondary antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
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bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
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bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Genomics 2019Quote: ... SureSelect Human All Exon V6 (Agilent), and the Human Core Exome Kit + RefSeq V1 (Twist) ...
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bioRxiv - Pathology 2019Quote: ... and mouse anti-human CD31 (DAKO) was applied overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Universal Human Reference RNA (UHRR, Agilent) was spiked into each of the samples for a final RNA amount of 300 ng to balance the RNA concentrations in the pools ...
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bioRxiv - Neuroscience 2019Quote: ... total tau (total human tau, Agilent), Tau-1 (Millipore) ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... SureSelect Human All Exon V6 (Agilent) exome libraries were prepared and samples were paired-end whole exome sequenced (WES ...
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bioRxiv - Neuroscience 2022Quote: ... and CD68 (Monoclonal Mouse Anti-Human, EDTA Buffer HIER, IHC dilution 1:5000, IF dilution 1:500, Clone L26, Dako Omnis, Code Number: M0756) were employed to characterize lympho-monocytic infiltrations ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of tumor DNA extracted from fresh-frozen tissue and 1 µg of male human reference DNA control (Agilent Technologies, Santa Clara, CA) were digested with AluI and RsaI ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Genetics 2020Quote: Trio WES of 13 patients and their parents was performed using the SureSelect Human All Exon V5+UTR kit (Agilent technologies). In patient 4 ...
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bioRxiv - Genomics 2019Quote: Paired-end DNA sequencing libraries of 28 individuals were generated using Aglilent SureSelect Human All ExonV6 kit (Agilent Technologies, CA, USA) by Novogene Co. ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was fragmented into 180–280 bp by sonication and subjected to library preparation using the Agilent SureSelect Human All Exon V6 Kit (#5190-8864, Agilent Technologies). The enriched libraries underwent paired-end 150bp sequencing on the Illumina HiSeq 2000 platform.
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Developmental Biology 2019Quote: ... Capacity for three-dimensional human blood vessel formation was quantified two weeks later using human CD31 (DAKO) immuno-histochemistry of harvested Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and pentose phosphate pathways were analyzed using the ultra-performance liquid chromatography (Agilent 1290 Infinity LC) coupled with triple quadrupole mass spectrometry (5500 QTRAP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
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bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: Mutagenesis of His 359 and Glu 363 to Alanine in human MYC (MycHEA mutant) was performed using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies # 200519), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole-exome sequencing libraries were prepared using SureSelectXT Human All Exon V5 + UTR kit (75 Mb; Agilent technologies, Santa Clara, CA, USA) as per manufacturer’s instructions and sequenced on HiSeq 2000 to generate paired end 2 × 100bp sequence reads (Illumina ...
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bioRxiv - Genetics 2023Quote: Exome enrichment was applied to the cDNA purified from the previous step by using probe kit SSELXT Human All Exon V8 (Agilent, 5191-6879) for human samples ...
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Molecular Biology 2022Quote: ... Accuscript reverse transcriptase (6×10−5) (Agilent, product literature), and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5 ...