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Citations for Agilent :
501 - 550 of 745 citations for Human Differentiation Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: anti-keratin 20 (KS.20.8, mouse monoclonal anti-human; Agilient-Dako), anti-PCNA (PC-10 ...
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bioRxiv - Biochemistry 2024Quote: K125R and K125E mutations of human connexin 26 were prepared using the QuikChange II mutagenesis kit (Agilent) and the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Bioengineering 2019Quote: ... The microtissues were then washed in PBS (3 × 1 hour) before mounting on glass slides using fluoro-safe mounting media (Dako, S3023). Mounted samples were allowed to cure for 1 day and then imaged using a confocal microscope (Zeiss LSM700 Confocal Microscope).
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bioRxiv - Cell Biology 2021Quote: ... washed three times in PBST containing 10 µg/ml DAPI for 5 minutes and mounted with a coverslip in fluorescent mounting media (Dako, S3023). All images were taken using a Zeiss LSM 710 confocal microscope and quantified manually using ImageJ software ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was labeled with Hoechst (1:2000) for 3 min and the sections were mounted using fluorescence mounting media (Dako, S3023). All images were taken using a Zeiss Observer Z1 fluorescent microscope using a 10X objective ...
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bioRxiv - Microbiology 2022Quote: ... Excess dye was removed by media change and the fluorescence at ex/em 574/594 was measured on a Biotek Neo plate reader (Agilent Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... and proton efflux rates (OCR/ECAR/PER) in media immediately surrounding adherent cells cultured in an XFe24-well microplate (Seahorse Bioscience). One day before analysis ...
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bioRxiv - Microbiology 2023Quote: ... all cultures were diluted in EMM(561 mM NH4Cl)+Leu+Ade+ura to seed an OD600nm at 0.01 into a poly-lysine coated Seahorse XFp cell culture miniplate in 50 μL of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were mounted onto glass slides with a coverslip (thickness:0.13-0.17 mm, Hounisen) and mounting media (DAKO, S3023, Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... 0.75 μg labeled cRNA was fragmented and hybridized to custom whole-genome human 8 × 60K microarrays (Agilent-048908) according to the supplier’s protocol (Agilent Technologies) ...
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bioRxiv - Pathology 2022Quote: ... the hydrogels were incubated overnight with a mouse anti-human α-smooth muscle actin antibody (DAKO, Glostrup, Denmark) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 90 of human ATG12 were mutagenized into arginine using site-directed mutagenesis following the manufacturer’s instructions (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... target enrichment was performed using the Agilent SureSelect Human All Exon V6 design (Agilent, Santa Clara, CA, USA).
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bioRxiv - Genetics 2019Quote: ... Total human brain RNA was derived from post mortem cortex and provided by Agilent (Agilent Technologies, cat: 540143). For the preparation of mouse brain RNA ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako, M0823, 1:100) and Alexa488-conjugated goat anti-mouse secondary antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: Two-channel microarray experiment was performed with two replicates using the Agilent Whole Human Genome Oligo Microarray (Agilent, catalog #G4851C Whole Human Genome Microarray 8×60K) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA of tumor and matched germline samples were captured using the SureSelect Human All Exon v7 (Agilent) or xGen Exome Research Panel v2 (IDT) ...
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bioRxiv - Biochemistry 2022Quote: Aliquots of 20 µL of human plasma were depleted on a 1260 Infinity Bio-inert LC system (Agilent) coupled to a depletion column (human 14 multiple affinity removal column ...
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bioRxiv - Genomics 2021Quote: ... Exome capture was performed using a SureSelect Human All Exon V6 capture kit (Agilent Technologies, Santa Clara, CA). Samples were sequenced on a HiSeq 4000 next-generation sequencing system (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide microarrays for human whole genome (G4858A design 072363, 8×60k chips SurePrint G3 unrestricted GE, Agilent Technologies) were used for global gene expression analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 2009). MEKK1 542–888 construct (H. sapiens MEKK1 TOG) was amplified from the MegaMan human transcriptome library (Agilent). The TOG construct was cloned into pETNKI-his-3C-LIC-kan vector ...
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bioRxiv - Molecular Biology 2019Quote: ... The R1285Q mutant of human FANCI (FANCImut) was generated by using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), and was cloned with a N-terminal cleavable Flag tag into the pCDNA 3.1 vector ...
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bioRxiv - Genetics 2019Quote: ... and exome DNA was enriched with an Agilent SureS elect Human All Exon V5 kit (Agilent Technologies, USA). Paired-end sequencing was performed on Illumina HiSeq 4000 (Supplementary Table 1 and 2).
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bioRxiv - Physiology 2021Quote: Mutations were introduced into human Cx50 cDNA in a SP64T vector with QuikChange Site-Directed Mutagenesis Kit (Stratagene) by using PCR primers that amplified the whole plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and incubated with horse radish peroxidase (HRP) conjugated goat-anti-human IgG antibody (Dako, P0447) (diluted 1:1,600 in PBS/0/1% BSA ...
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bioRxiv - Genetics 2020Quote: ... The whole exome was captured by an Agilent SureSelect All Human exon v5.0 kit (Agilent, Santa Clara, US.), and sequenced on an Illumina Hiseq X-10 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
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bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Cancer Biology 2023Quote: ... which recognises residues 1978-2016 of human NaV1.5) and stained with EnVision+ Dual Link System/HRP (DAB+; Dako), following the manufacturer’s instructions and counterstained with Mayer’s haematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Quick Amp Labeling Kit and SurePrint G3 Human Gene Expression 8×60Kv3 Microarray (Cat. No. G4851C, Agilent Technologies) was used corresponding to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Immunology 2023Quote: ... Positive control PhIP-seq designed to capture mouse and human GFAP began by incubating 0.1µg/mL polyclonal anti-GFAP antibody (Dako) with the murine PhIP-seq library.
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The sequence library was prepared using a Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced using a 2500 Illumina with 125-bp paired-end reads (Illumina ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Immunology 2022Quote: ... 3×105 cells from each donor were plated per replicate in DMED media adjusted to a pH of 7.4 in a p96 well plate (Seahorse XFe96 FluxPak Agilent Technologies 102416-100) in the presence of 0.125 mM Sodium pyruvate ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were finally washed for 3 × 10min with PBS before mounting them on glass coverslips with Dako fluorescence conserving media (Dako, Hamburg, Germany).
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Neuroscience 2024Quote: ... fibroblasts were seeded at 45 × 103 cells per well (5-10 replicates per cell line) in fibroblast growth media in a Seahorse XFe24 Microplate (Agilent, 100777-004) and incubated for 24 hours to allow cells to adhere ...
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bioRxiv - Cancer Biology 2023Quote: ... (Clones #87 and #160) cultured in COMMA1D media were seeded in 22 wells of a Seahorse 96-well plate (Agilent #103792-100) at a density of 15,000 cells per well ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed in PBS 5 x 5 mins, then mounted with a cleaned glass coverslip (#1.5, 24 mm x 60 mm) in mounting media (Dako; Pathology Products, S3023).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genomics 2019Quote: ... 37,608 individual biotinylated RNAs targeting the ends of 22,076 promoter-containing human HindIII restriction fragments) were prepared according to the manufacturer’s instructions (SureSelect Target Enrichment, Agilent Technologies). The Hi-C library DNA was denatured for 5 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...