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Citations for Agilent :
1 - 50 of 6231 citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HA-RAMP3ΔPDZ and SNAP-RAMP3ΔPDZ constructs were generated by removing the DTLL PDZ recognition sequence through site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis and SNAP-RAMP and Nluc-GPCR generation was confirmed by Sanger sequencing (Department of Biochemistry ...
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bioRxiv - Biochemistry 2019Quote: ... The CFTR PDZ-binding motif (CFTR residues 1477-1480) was inserted downstream using Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... and programmed cell death ligand 1 (PD-L1; clone 22C3, pharmDx kit, Agilent, USA) were used ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding the ACR-16 TM3-4 loop or the SDN-1 intracellular domain full length or lacking the EYFA PDZ binding motif were cloned to pGEX-3X expression vector and then transfected into Arctic express E.coli strain (Agilent). Bacterial clones were first cultured overnight in 5 mL LB medium supplemented with 100 mg/mL ampicillin ...
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bioRxiv - Neuroscience 2023Quote: ... neuronal media was changed to XF DMEM (Agilent) containing 1mM pyruvate ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Cell Biology 2020Quote: Citrate Synthase Normalization Assay was performed on Seahorse XF96 Cell Culture Microplate (Agilent) right after seahorse assay or on plates frozen at −80°C with leftover medium aspirated ...
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bioRxiv - Immunology 2021Quote: ... cells were sequentially injected with 1.5μM oligomycin A (ATP synthase inhibitor; Agilent Technologies), 0.5μM FCCP (mitochondrial uncoupling agent ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... SureSelectXT Human All Exon Kits (Agilent) or Human Core Exome (Twist Bioscience ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Biophysics 2022Quote: ... ligand content was analyzed using an HPLC system (Agilent 1100 Series) and samples were separated using a Zorbax Eclipse XDB-C18 column (Methanol 70% isocratic condition ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Neuroscience 2023Quote: ... neuronal cells were plated in a XF96 cell culture microplate (Agilent Technologies) at a density of 70,000 cells/well ...
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bioRxiv - Genomics 2019Quote: ... Library construction (Agilent SureSelect Human All Exon kit), quality assessment ...
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bioRxiv - Genomics 2019Quote: ... SureSelectXT Human all exon V6 kit (Agilent Technologies) was used to capture exons ...
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bioRxiv - Cancer Biology 2021Quote: ... A SureSelectXT Human All Exon V6 kit (Agilent) was used for exome capture ...
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bioRxiv - Bioengineering 2023Quote: ... using a 1.5% w/v alginate solution in deuterium oxide (64 scans; Agilent 400 MHz Premium COMPACT equipped with Agilent OneNMR Probe ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Library preparation (Agilent SureSelect Human All Exon V6 kit) and high throughout sequencing (Illumina NovaSeq 6000 ...
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Cancer Biology 2024Quote: ... The Agilent SureSelect Human All Exon v6 kit (Agilent Technologies) was used for whole exome sequencing ...
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bioRxiv - Genetics 2024Quote: ... The exomes of the subjects were captured by Agilent SureSelect Human All Exon V6 Enrichment kits (Agilent, CA, USA) and then sequenced on a HiSeq X-TEN system (Illumina ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Cell Biology 2021Quote: ... The N-terminal region was removed by a Quikchange reaction (Agilent), and the resulting shortened fragment was inserted into the pDEST-HisMBP destination vector via a Gateway reaction.
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bioRxiv - Cancer Biology 2019Quote: Whole Human Genome Microarray Kit 4×44K (Agilent, Cat. No. G4112F) was used to detect mRNA expression levels in cells transfected with control and miR-101-3p transfected HCT116 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Exomes were sequenced using the AllExon Human SureSelect v7 Kit (Agilent).
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bioRxiv - Genomics 2021Quote: ... The Agilent SureSelect Human All ExonV5 Kit (5190-6209, Agilent Technologies) was used for exome capture according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... using a SureSelect Human All Exome V6 capture kit (Agilent, USA) or SeqCap EZ MedExome Enrichment Kit (Roche ...
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bioRxiv - Genetics 2023Quote: ... The Agilent SureSelect Human All Exon V6 Kit (Agilent Technologies, USA) was applied for exon capture ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in mito stress test media with or without ligand(s) (XF base media (Seahorse Bioscience) supplemented with 1mM sodium pyruvate (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin was determined with ELISA (Dako Denmark) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Exome capture was performed with the SureSelect Human All Exon v6 kit (Agilent) and sequenced on the Illumina HiSeq platform ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing were generated using SureSelect Human All Exon 50Mb Kit (Agilent Technologies) coupled with Illumina HiSeq sequencing system (Illumina) ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were generated using Agilent SureSelect Human All Exon kit (Agilent Technologies) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample (experimental details provided in the Supplementary Data).
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bioRxiv - Neuroscience 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... the neuronal maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 5 mM glucose and 1 mM pyruvate ...
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bioRxiv - Biophysics 2022Quote: TRPV4 N-terminal constructs were expressed in Escherichia coli BL21-Gold(DE3) (Agilent Technologies) grown in terrific broth (TB ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... A secondary quantification measurement with Bioanalyzer Protein Analysis Kits (Agilent) provided similar concentration values as SYPRO Red assay (data not shown).
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualised using DAB (Dako Real Envision kit) for 3-5 minutes ...
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bioRxiv - Neuroscience 2023Quote: Neuronal mitochondrial function following vehicle or cytokine treatment was characterized on the Seahorse XFe24 Extracellular Flux Analyzer platform (Agilent). The Seahorse Analyzer measures oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2020Quote: The one-color microarray Human miRNA Microarray Kit (V2) design ID 029297 from Agilent Technologies was used to measure miRNA expression for 425 tumors of the Oslo2 cohort using 100 ng total RNA as input ...
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bioRxiv - Bioengineering 2022Quote: ... The CGH assays were performed using the SurePrint G3 Human CGH Microarray Kit (Agilent) and the genomic DNA of WTC11 cells as a reference of the diploid cells ...