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Citations for Agilent :
301 - 350 of 6818 citations for HDM Fluorescent Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Developmental Biology 2023Quote: ... Sure 2 (Agilent) competent cells were transformed with the ligation product and cultured at reduced temperatures (27°C) ...
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bioRxiv - Physiology 2023Quote: ... The absorbance of the plate was read at 570 nm using a plate reader (Synergy H1, Agilent BioTek). Fat body TAG content was determined as described above ...
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bioRxiv - Immunology 2019Quote: ... Seahorse 96 well assay plates (Seahorse Bioscience) were coated with Cell-Tak suspension (Corning) ...
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bioRxiv - Physiology 2021Quote: Cells seeded in Seahorse plates (Agilent Technologies) were washed with assay medium (Seahorse XF DMEM supplemented with 6mM glucose ...
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bioRxiv - Immunology 2020Quote: ... Plates were developed using TMB (Dako, S1599) and neutralized with 2N H2SO4 ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded in XFp plates (Agilent) at either 1.5×103 cells/well the day before experiment when growing in CPT medium ...
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bioRxiv - Immunology 2022Quote: ... and a well-plate autosampler (#G1367A, Agilent). Peptides were first loaded onto a 4 cm trapping column (made in-house ...
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bioRxiv - Pathology 2023Quote: ... A BioTek Synergy H1 plate reader (Agilent) with Gen5 software (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... using a Synergy H1 plate reader (Agilent).
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bioRxiv - Microbiology 2024Quote: ... Plates were imaged on a Cytation7 (Agilent), and foci were counted using Gen5 software ...
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bioRxiv - Biophysics 2022Quote: ... as fluorescent probes to detect the exposed hydrophobic residues by an MX3005 qPCR instrument (Agilent, Santa Clara, USA). Here ...
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bioRxiv - Genomics 2021Quote: ... The fragment size (ca. 100 nucleotides) was verified by fluorescent capillary electrophoresis using an Agilent 2100 Bioanalyzer (Agilent RNA 6000 pico kit ...
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bioRxiv - Neuroscience 2021Quote: ... Hoechst 33342 was used to stain the nucleus before cells were mounted with fluorescent mounting medium (DAKO, #S3023). The following primary antibodies were used ...
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bioRxiv - Genetics 2023Quote: ... slides were carefully rinsed 3 × 5 min with PBS and slides were mounted with Fluorescent Mounting Medium (Dako) and examined under fluorescence using a Zeiss microscope equipped with an AxioCam HRm camera.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times with PBS and mounted using fluorescent mounting medium for imaging (Dako, #S3023).
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bioRxiv - Neuroscience 2024Quote: ... diluted in PBS for 10 min at RT and accordingly mounted using DAKO fluorescent mounting medium (DAKO; S3023). Zeiss Axio Imager Z1 was used to image the samples ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted onto positively charged glass slides and coversliped using DAKO fluorescent mounting medium (Agilent Technologies S3023). A negative control (i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... and 2-deoxy-d-glucose (50 mM 2-DG, Agilent Technologies) as a glycolysis inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’-diaminobenzidine (DAB) substrate (Agilent Cat#GV82511-2 or GV92511-2), Dako REAL Peroxidase-blocking reagent (Agilent S202386-2) ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
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bioRxiv - Microbiology 2020Quote: ... DNA removal was confirmed by PCR using primers OL398 and OL399 (Table 2) and RNA quality was assessed using an Agilent 2100 Bioanalyzer system with corresponding RNA 6000 Nano kit (Agilent) to confirm RNA integrity (RIN) ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Mutation of glycine at position 2 to alanine was accomplished using the Quick-Change Mutagenesis kit (Agilent, Santa Clara, CA) to generate ptubTgFBXO1HAG2A.
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: About 2 µg of obtained total RNA was immediately used for cDNA formation using AccuScript High Fidelity cDNA Synthesis Kit (Agilent). RT-qPCR was performed in a 96-well plate on a CFX96 qPCR system (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg DNase-treated RNA was reverse transcribed using a cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), following the respective manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 the XF Real-Time ATP Rate Assay Kit (Cat. No. 103592-100, Agilent Technologies, Cedar Creek, TX) was run following the manufacturer’s protocol using the Seahorse XF96 Analyzer as described above.
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bioRxiv - Biochemistry 2021Quote: DSF experiments were done in a 96-well plate in a plate reader combined with a thermocycler (Stratagene Mx3005P). All hBrr2 constructs were diluted to a final concentration of 3 μM in purification buffer (± 2 mM ATP/Mg+2 ...
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bioRxiv - Microbiology 2023Quote: Vero TMPRSS2 cells were plated in 96-well tissue culture treated E-Plate VIEW plates (#300-601-020, Agilent) to be subconfluent at time of assay ...
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bioRxiv - Developmental Biology 2019Quote: ... DAPI (1:500, Dojindo) was used for nuclear staining and the sections were mounted with fluorescent mounting medium (Dako). At least three embryos of each genotype were used for each analysis.
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bioRxiv - Cell Biology 2019Quote: ... was used to visualize nuclei and sections were mounted using an aqueous medium (Cytomation fluorescent mounting Medium, S3023, Dako). All images were visualized using an Olympus BX60 microscope (Olympus Optical ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were finally washed three times with PBS and mounted with fluorescent mounting media (Dako, Les Ulis, France). Slides were examined under fluorescence using a microscope (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with DAPI (1:500 in 0.1M PBS) and mounted on glass slides using fluorescent mounting media (DAKO) and examined in a fluorescence Observer Z1 microscope with ApoTome 2 under a Plan-Apochromat 40x/1.3 Oil DIC objective (Carl Zeiss) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with PBS and mounted on glass - slides using fluorescent-DAKO mounting media (Agilent Technologies). Images of tubulin morphology and costainings of tubulin and Par3 were captured at the Sanford Burnham Prebys Cell Imaging Core ...
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bioRxiv - Bioengineering 2020Quote: ... inserts were transferred to a new clear bottom plate where fluorescence was immediately measured using a bottom reading fluorescence plate reader (BioTek, Agilent) at 485/535 nm (Ex/Em) ...
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bioRxiv - Biophysics 2022Quote: ... or aged samples (T ~ 24 hours) were imaged directly in sealed 384-well microwell plates with a BioTek Cytation Gen 5 imaging plate reader (Agilent) using a 20 x objective ...
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bioRxiv - Immunology 2021Quote: ... the utility plate without cartridge was unloaded and the cell plate was loaded into the XF24 analyser (Seahorse, Agilent Technologies). After equilibration ...
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bioRxiv - Neuroscience 2022Quote: ... The lysis samples were transferred from four 96-well plates to each 384-square-well plate using the 96-well module-coupled VPrep liquid handler (Agilent) with 30 μL tips (twice without changing tips) ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed between all experimental step with PBS plus 0.1% Tween 20 (405 TS ELISA Plate Washer, Agilent Technologies). Blocking (1% Omniblok ...
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bioRxiv - Biochemistry 2023Quote: ... The purified peptide binders and target peptides were titrated in the presence of Nano-Glo substrate in 96-well plates and the luminescence was measured on a Synergy Neo2 plate reader (Agilent). To conduct the fluorescence polarization binding assays ...
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bioRxiv - Microbiology 2023Quote: Log phase cultures of cells grown without inducer were diluted to an OD600 of 0.05 and grown in 96-well plates for 24 h at 30°C with constant shaking on a BioTek Cytation 1 plate reader (Agilent), measuring the absorbance at 600 nm every 30 min for three technical replicates for each strain ...