Labshake search
Citations for Agilent :
1 - 50 of 8447 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
-
bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
-
bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
-
bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
-
bioRxiv - Bioengineering 2019Quote: ... S-100 (1:250, Dako), and/or (v ...
-
bioRxiv - Neuroscience 2020Quote: GST-NT and GST-NT-ABmut were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent, 230280), purified as described previously (Fujiwara et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... Immunostaining for S-100β (DAKO, Z0311, 1:500) was performed in 1X PBS containing 0.2% Tween 20 (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... Point mutations on GST-dyn1xA-PRR were generated using the QuickChange site-directed mutagenesis kit (Stratagene) and were cloned into pGEX-6P-1 vector (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
-
bioRxiv - Genetics 2020Quote: For pull-down experiments each recombinant version of His6-GST-tagged Sen1 Cter or the His6-GST control was overexpressed by growing BL21 (DE3) CodonPlus (Stratagene) cells harboring the appropriate plasmid (see table S13 ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-PALB2 full-length and fragments were purified from 1 L of ArcticExpress cells (Agilent Technologies), grown at 37°C in LB broth medium containing 50 µg/mL ampicillin and 25 µg/mL gentamycin ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were analyzed on a fluorescent plate reader (Biotek Synergy HTX, Agilent Technologies, Inc., Santa Clara, CA) at excitation of 485/20 nm and emission 528/20 nm ...
-
bioRxiv - Cell Biology 2022Quote: ... MD and mutated at AKAP12’s activation-responsive sites (S/T to A mutations) using the QuikChange II® site-directed mutagenesis Kit (Stratagene, CA) as we described previously (54) ...
-
bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
-
bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S ...
-
bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel ...
-
bioRxiv - Cancer Biology 2020Quote: ... GST-CD1 DENN V226K/I228D/L235D/Y238K and GST-CD1 DENN M243R/P244R/Q362A were generated using the QuickChange lightning site-directed mutagenesis kit from Agilent Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: Glutathione Sepharose 4B resin and Calmodulin Affinity Resin (Agilent Technologies, 214303) were washed twice with Blocking Buffer (20 mM Hepes pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... PLRP-S columns (Agilent) were used for protein conjugates with similar acetonitrile/water/0.05% TFA gradients ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was labelled with Cy3 (ULS™ Fluorescent Labeling Kit for Agilent Arrays (EA-023), Kreatech ...
-
bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
-
bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
-
bioRxiv - Cancer Biology 2021Quote: ... diluted 1:50 in antibody diluent with background reducing components (DAKO S 3022). A secondary antibody (HRP EnVision rabbit (DAKO K4003 ...
-
bioRxiv - Cancer Biology 2022Quote: ... EZH2’s SET domain deletion was generated by a site-directed mutagenesis kit (Agilent, 200521). All plasmids were verified by Sanger sequencing ...
-
bioRxiv - Physiology 2021Quote: ... in fluorescent mounting medium (Dako). The number of insulin+ β-cells were counted in a random manner by a single observer using a microscope (Olympus Corp.) ...
-
bioRxiv - Cell Biology 2020Quote: ... Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene). Details of the expression and purification protocol have been described previously [59 ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR-amplified DNA was cloned into the GST-pGEX-2TK plasmid (Stratagene). GST-FHA fragment was expressed into E.coli BL21PlysS at 30°C for 4h ...
-
bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Slides were mounted with fluorescent mounting medium and analysed with fluorescent mounting medium (Dako Agilent).
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Slides were mounted with fluorescent mounting medium and analysed with fluorescent mounting medium (Dako Agilent).
-
bioRxiv - Cancer Biology 2023Quote: ... All reagents for immunohistochemistry were from Dako (Dako, Glostrup, Denmark A/S). Appropriate positive and negative controls were included for each run of IHC.
-
bioRxiv - Developmental Biology 2022Quote: ... mounted with fluorescent Mounting Medium (DAKO) and stored at 4°C protected from light until image acquisition.
-
bioRxiv - Developmental Biology 2021Quote: ... mounted in Fluorescent Mounting Medium (Dako), and stored at 4°C in the dark.
-
bioRxiv - Neuroscience 2022Quote: ... Then fluorescent mounting medium (S3023, Dako) was applied to brain sections and covered by a cover glass ...
-
bioRxiv - Immunology 2021Quote: ... 1:2000 HRP-conjugated goat anti-rabbit polyclonal antibody (Dako, Denmark A/S, Cat#P0448) was added and the plate was incubated at 37°C for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Goat Anti-Rabbit Immunoglobulins/HRP (dilution 1:100,000, #P0448, Dako Denmark A/S, Glostrup, Denmark) for 1h in RT ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleus staining was performed with DAPI 1 µg/mL for 1 min and coverslips were mounted on slides with fluorescent mounting medium (Dako). Cells were observed under a SP8 confocal laser-scanning microscope equipped with an HCP PL APO 100x/1.44 Oil CORR CS immersion objective (Leica) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Citrate pH 6.1 (DAKO, S-1700) or Antigen Unmasking Solution ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 Å pore PLRP-S (Agilent) beads ...
-
bioRxiv - Cancer Biology 2022Quote: ... All sections were counterstained with nuclear DAPI (1:1000) and mounted with fluorescent mounting medium (Dako).
-
bioRxiv - Cell Biology 2022Quote: ... GST-TNY constructs were transformed into E.coli BL21 codon plus-RIL strain (Agilent Technologies). Cells were grown in LB media and induced for 5 hrs at 30°C with 0.5 mM isopropyl-β-d-thiogalactopyranoside (IPTG ...
-
bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
-
bioRxiv - Genomics 2023Quote: ... CD20, Ventana Roche L26, prediluted; CD3, Leica LN10, 1:500; CD68, DAKO A/S PG-M1, 1:50; CD138, DAKO A/S ...
-
bioRxiv - Microbiology 2020Quote: ... and Sialic acids from the purified S-Ectodomain was performed by using a Deglycosylation kit (Agilent, CA, USA) as per the manufacturer instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... and mounted with fluorescent mounting medium (Dako).
-
bioRxiv - Cell Biology 2022Quote: ... Dako fluorescent mounting medium from Agilent (S3023) was used for mounting coverslips onto glass slides.
-
bioRxiv - Developmental Biology 2022Quote: ... slides were mounted (DakoCytomation fluorescent mounting medium) and imaged using a Zeiss AxioObserver fluorescence microscope equipped with an Apotome 2 ...
-
bioRxiv - Neuroscience 2023Quote: ... using fluorescent mounting medium (Dako, Ref S3023). Immunofluorescence images were taken with an AxioImager Z1 microscope equipped with Apotome (Zeiss).
-
bioRxiv - Developmental Biology 2024Quote: ... slides were mounted (DakoCytomation fluorescent mounting medium) and stored at 4°C until imaging.