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Citations for Agilent :
1 - 50 of 693 citations for Galectin 9 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used were: mouse anti-human CD8 (Dako, clone C8/144B, 1:800, pH 9 retrieval), mouse anti-human CD68 (Dako ...
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bioRxiv - Molecular Biology 2022Quote: contains the canonical human HELZ2 long isoform cDNA (2649 bps) cloned into pCMV-3Tag-9 (Agilent Technologies), which allows the expression of a HELZ2-3xMYC fusion protein ...
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bioRxiv - Neuroscience 2020Quote: GST-NT and GST-NT-ABmut were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent, 230280), purified as described previously (Fujiwara et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... pH 9 (Dako) and endogenous peroxidase activity was blocked using 3% hydrogen peroxide ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Cell Biology 2022Quote: ... pH 9 (Dako Agilent) at 97°C from 10 min and cooled down to 65°C ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 9 (Dako, S2367) in a microwave for 25 min ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 9 (Agilent S2367). The chamber was heated to 125 °C ...
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bioRxiv - Cell Biology 2022Quote: ... pH 9 (Dako Agilent) at 97°C from 10 min and cooled down to 65°C ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 9 (both Dako), endogenous peroxidase activity was blocked using 3% hydrogen peroxide and sections were blocked in PBS with 10% FBS (blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 9 (Agilent, #S2375) were heated to 97 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 9 (Agilent, S2367) for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 9 (Agilent, S2367). 400µl/slide of primary antibody was incubated on slides overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 9 (Dako, S2367) at 115°C for 15 minutes in a pressure cooker for antigen retrieval ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 9 (Dako, S2367) with a pressure cooker for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
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bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
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bioRxiv - Genetics 2020Quote: For pull-down experiments each recombinant version of His6-GST-tagged Sen1 Cter or the His6-GST control was overexpressed by growing BL21 (DE3) CodonPlus (Stratagene) cells harboring the appropriate plasmid (see table S13 ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Systems Biology 2020Quote: ... pH 9 (Agilent, S236784-2) for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 9 (Agilent, #S236784-2) at 97 °C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 9 solution (Dako, S2367) in a pressure cooker at 125°C for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
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bioRxiv - Cancer Biology 2019Quote: ... pH 9 (S2367, Dako, Glostrup, Denmark) in a microwave oven for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 9 (Agilent Technologies, Santa Clara, CA). The sections were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
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bioRxiv - Genomics 2019Quote: ... Androgen Receptor (Dako, M3562, 1/200, pH 9). Slides were digitalized using a Hamamatsu NanoZoomer slide scanner (Japan ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 9 (S236784-2, DAKO, Carpinteria, CA, USA), for 20 min in a microwave oven ...
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bioRxiv - Cell Biology 2020Quote: ... Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene). Details of the expression and purification protocol have been described previously [59 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified DNA was cloned into the GST-pGEX-2TK plasmid (Stratagene). GST-FHA fragment was expressed into E.coli BL21PlysS at 30°C for 4h ...
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bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
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bioRxiv - Neuroscience 2019Quote: ... and with Target Retrieval Solution (pH 9, Agilent, Dako) for the poly(GP ...
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bioRxiv - Neuroscience 2019Quote: ... and with Target Retrieval Solution (pH 9, Agilent, Dako) for the poly(GP ...
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bioRxiv - Bioengineering 2019Quote: ... and Ki-67 (DakoCytomation clone 39-9, Glostrup, Denmark) (dilution 1/50 ...
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bioRxiv - Immunology 2021Quote: ... using D5000 screentape (Agilent, Cat no. 5067-5588/9).
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bioRxiv - Developmental Biology 2023Quote: ... RNAs were quality assessed (Agilent 2100 Bioanalyzer, RIN>9) and RNA libraries were prepared using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Pathology 2023Quote: ... Sections from the first level (main experiment) were stained for smooth muscle alpha-2 actin (ACTA2) and galectin 3 (LGALS3) using mouse monoclonal anti-ACTA2 (Dako, cat. no. M0851, 1:100) after blocking with Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2022Quote: ... and RNA integrity values of 9-10 (Bioanalyzer, Agilent Technologies). Gene-specific primers and dual-labelled probes (labelled with 6-carboxyfluorescein and BHQ-1 ...
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bioRxiv - Bioengineering 2022Quote: ... pAAV2/9 and a helper plasmid (Stratagene, La Jolla, CA) as previously described92 ...
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bioRxiv - Neuroscience 2019Quote: ... samples for RNA-seq had RIN values >9 (BioAnalyzer, Agilent). 500 ng of purified RNA was used to prepare libraries for sequencing using the Truseq m RNA l ibrary prep kit (I l lumina RS-122-2001/2) ...
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bioRxiv - Cell Biology 2022Quote: ... GST-TNY constructs were transformed into E.coli BL21 codon plus-RIL strain (Agilent Technologies). Cells were grown in LB media and induced for 5 hrs at 30°C with 0.5 mM isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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bioRxiv - Microbiology 2021Quote: ... and the AEC substrate 3-amino-9-ethylcarbazole (Dako, Carpinteria, CA). Moreover ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity number (≥9) was determined by electrophoresis (Tapestation, Agilent). Stranded libraries were prepared from 500 ng total RNA input with poly-A+ selection (TruSeq Stranded mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was done using pH 9 (Dako, S2367, CA, USA) solution in a pressure boiler (BioCare medical Model DC2008INTL ...
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bioRxiv - Cell Biology 2022Quote: ... The expression of the GST-GAP fusion protein in E.coli strain BL-21-RILP (Stratagene) and its purification using Glutathione Sepharose 4B were performed as described previously (Jung et al. ...