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Citations for Agilent :
1 - 50 of 52 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... amide HILIC column (Agilent Glycan Mapping ...
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bioRxiv - Molecular Biology 2022Quote: Glutathione Sepharose 4B resin and Calmodulin Affinity Resin (Agilent Technologies, 214303) were washed twice with Blocking Buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The amino acids were derivatized with OPA for primary amino acids and FMOC for secondary amino acids as per the protocol provided by Agilent. Derivatization was performed on the autosampler ...
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bioRxiv - Physiology 2023Quote: ... equipped with a 100 x 2.1 mm XBridge amide column (Waters) coupled to a 6490 Triple Quad MS (Agilent Technologies) in negative ionization mode via multiple reaction monitoring (MRM ...
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bioRxiv - Biochemistry 2020Quote: ... Calmodulin Affinity Resin (Agilent Technologies) was added to the soluble extract and the sample incubated for 90 minutes at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The C18 resin beads (Agilent) were first washed with 50% ACN solution and then equilibrated with 0.5%TFA in 5% ACN solution ...
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bioRxiv - Neuroscience 2023Quote: ... Strataclean resin (Agilent-400714-61), Bafilomycin A1 (Sigma-Aldrich-B1793) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was measured using an XBridge Amide 3.5 μm, 4.6 × 150 mm column (Waters, Milford, MA) on a 1220 Infinity II HPLC (Agilent, Santa Clara, CA). The mobile phase was water/acetonitrile 20:80 to 60:40 at a flow rate of 1.0 mL/min for 6 min ...
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bioRxiv - Biochemistry 2024Quote: ... using a 2.1 mm × 10 mm Acquity BEH Amide Column (1.7 μm particle size) (Waters, Elstree, UK) attached to an Agilent 1260 Infinity II (Agilent, Manchester, UK). Mobile phase was solvent A ...
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bioRxiv - Plant Biology 2020Quote: ... concentrated with StrataClean resin (400714, Agilent) and analyzed using Western blot method (α-GFP ...
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bioRxiv - Microbiology 2022Quote: ... 50 μl of StrataClean resin (Agilent) was added to each sample before vortexing for 1 minute ...
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bioRxiv - Microbiology 2021Quote: ... 0.001% (v/v) of StrataClean resin (Agilent) was added in order to bind protein ...
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bioRxiv - Biochemistry 2023Quote: ... BBP-Mud2 dimer and calmodulin resin (Agilent). After incubation at 4°C for 3 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The final eluate was concentrated with StrataClean resin (Agilent) before extracted using 2X SDS buffer and separated in a 10% SDS-PAGE gel (Bio-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: ... PLRP-S resin (~10-μm mean particle diameter; Agilent) was functionalized with a non-cleavable linker by ChemGenes ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was concentrated using the StrataClean resin (Agilent Technologies).45 CM volume for StrataClean binding was adjusted to obtain 100µg of total protein ...
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bioRxiv - Biochemistry 2024Quote: ... Each fraction was incubated with 10μL StrataClean resin (Agilent) for 30min at 4℃ to concentrate the diluted proteins in each fraction ...
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bioRxiv - Biochemistry 2022Quote: ... or a FLAG tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) was added on the N-terminus of MBP WT or R919* TRPA1 using Quikchange Lightning site-directed mutagenesis (Agilent).
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bioRxiv - Molecular Biology 2020Quote: ... and bound to 1.5 ml Calmodulin Affinity Resin (Agilent Technologies) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The elutions were incubated with calmodulin affinity resin (Agilent Technology) in buffer E plus 2 mM CaCl2 at 4 °C for 2 h and eluted in buffer E plus 10 mM EGTA.
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were further cleaned by C-18 resin columns (Agilent). (Cleanup and elution of peptides via C-18 column was performed as per the company protocols) ...
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bioRxiv - Plant Biology 2020Quote: ... protein concentrated from the liquid media with Strataclean resin (Agilent Technologies) after filtration was used as an extracellular fraction.
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bioRxiv - Genomics 2023Quote: PLRP-S resin (1000 A, 10-μm; Agilent Technologies, PL1412-4102) was used for the barcoded oligonucleotide synthesis ...
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bioRxiv - Genetics 2022Quote: ... 225 µL gradient fractions were collected and concentrated using StrataClean resin (Agilent). Concentrated fractions were eluted in 1.5X Laemmli buffer + 37.5 mM DTT and run on SDS-PAGE for immunoblotting.
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bioRxiv - Microbiology 2023Quote: ... Resin acid identification and quantification were performed by capillary GC (Agilent 7890A). Helium ...
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bioRxiv - Microbiology 2023Quote: ... tryptic peptides were bound to C18 resin (Bond Elut C18 cartridge, Agilent) in 0.1% TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... 75 μm inner diameter packed with 1.8 μm C18 resin (Agilent Zorbax SB-C18) at a constant temperature of 40°C ...
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bioRxiv - Biochemistry 2024Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
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bioRxiv - Immunology 2023Quote: ... proteins in the supernatant were precipitated with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... FACT was pulled down from the extract with 0.5 ml packed calmodulin affinity resin (Agilent) for 4 hours at 4°C with gentle rocking ...
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bioRxiv - Biochemistry 2020Quote: ... and the peptides desalted and concentrated upon C18 resin (OMIX C18 pipette tips; Agilent #A57003100) essentially as previously described (69) ...
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bioRxiv - Biochemistry 2021Quote: ... The eluates were pooled and applied to 1 ml of calmodulin affinity resin (Agilent Technologies). Beads were washed with 20 CV of buffer D / 1 mM DTT / 2 mM CaCl2 and bound protein eluted with 8 CV of buffer D / 1 mM DTT / 2 mM EGTA / 2 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to in solution-trypsin digest on 10µl of resin (StrataClean, Agilent, Cheshire, UK) followed by reduction and alkylation ...
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bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
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bioRxiv - Evolutionary Biology 2024Quote: ... peptides were purified and concentrated using OMIX Tips C18 reverse-phase resin (Agilent Technologies Inc.) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mL fractions were collected and protein was concentrated by incubation with 4μL StrataClean resin (Agilent Technologies) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Proteins were extracted from sucrose gradient fractions by incubation with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... proteins were extracted from the cytosol fraction by incubation with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... seawater was collected at 3m depth by a tow-fish and pumped at a flow rate of 250 ml min-1 through a 0.2 µm filter and a polytetrafluoroethylene column packed with 20 g of polystyrene resin (Bondesil ENV; Agilent). Each sample represents an integrated average of 400-600 L of water across a wide region ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was supplemented with 2 mM CaCl2 and applied to 2.4 mL bed volume (BV) Calmodulin-affinity resin (Agilent) pre-equilibrated in ORC lysis buffer containing 2 mM CaCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The enzyme was heat inactivated at 75°C for 2 minutes and then removed with 1μl of Strataclean resin (Agilent). Capped RNAs were precipitated using 2M ammonium acetate and isopropanol to remove any residual nucleotides ...
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bioRxiv - Physiology 2024Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described37 ...
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bioRxiv - Systems Biology 2021Quote: ... 30 μL sample were loaded to a ZORBAX Bonus-RP column (with 3.5 μm C18 resins, ID 2.1 mm, length 50 mm; Agilent Technologies, USA), and fractionated with a Agilent 1200 series HPLC System (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... CaCl2 was added to a final concentration of 2 mM and the supernatant was added to a disposable column containing 400 μL calmodulin resin (Agilent) pre-equilibrated with E buffer and 2 mM CaCl2 ...
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bioRxiv - Cancer Biology 2020Quote: EGFR-KD from Expi293 cell lysates was bound to streptavidin resin and eluted in biotin buffer according to manufacturer’s recommendations (InterPlay Mammalian TAP System; Agilent Technologies). Typical protein yield was between 100-400 ng/µl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptides were then purified and concentrated using OMIX Tips C18 reversed-phase resin (Agilent Technologies, Santa Clara, CA, USA). The peptides were dehydrated in a vacuum centrifuge and analysed by NanoLC-MS/MS ...
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bioRxiv - Microbiology 2023Quote: Pooled gradient fractions were diluted 1:2 with ice-cold PBS and tumbled overnight at 4°C with 50 µl Strataclean resin (Agilent). Beads were pelleted at 600 RCF for 5 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... dTRCs were extracted from the river and HZ water matrix using a solid phase extraction with Bondesil C18 resin (Agilent). Samples were passed over 8 ml of resin at the rate of 1 ml•min−1 ...