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Citations for Agilent :
1 - 50 of 573 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... IgA heavy chain (rabbit anti-human, Dako, A0262 1:1000) and secretory component/pIgR (goat anti-human ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain plasmids were mutated using the QuikChange Lightning (Agilent) kit ...
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bioRxiv - Pathology 2020Quote: ... smooth muscle myosin heavy chain (SMYOHC) were purchased from Agilent (Dako Corporation) for clinical in vitro diagnostics with appropriate dilutions and controls under catalogue numbers M730401-2 ...
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bioRxiv - Microbiology 2021Quote: ... Heavy chain Y58F or F58Y mutants were constructed using the QuikChange XL Mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Mutations in the wild type heavy chain plasmid were performed using site directed mutagenesis (Agilent, 200524) following the manufacturer’s protocol to generate EG ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The RM19R Fab was expressed in HEK293F cells (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Immunology 2022Quote: Specific amino acid changes on envelope clones and CAP206-CH12 heavy chain CDRH3 were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog #210519, Agilent Technologies, CA, USA). Mutations were confirmed by sequence analysis.
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bioRxiv - Immunology 2020Quote: ... followed by incubation with polyclonal antibodies against human kappa light chains (Dako, A0192) and by HRP-conjugated protein A (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Immunology 2021Quote: ... or IgA (α-chain specific, catalog no. P0216, Agilent) secondary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Polymerase chain reaction (PCR) reagents and kits were from Agilent and Himedia ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) was performed using Pfu Turbo (Stratagene).
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using SureCycler 8800 (Agilent) and AriaMx Real-Time PCR System (Agilent) ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reactions were performed using Pfu Ultra II polymerase (Agilent) or Clontech Hifi polymerase (Takara) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Nine heavy metal concentrations were determined using an air-acetylene FAAS (SpectrA 240B Agilent technologies, USA) equipped with deuterium background corrector ...
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bioRxiv - Biochemistry 2019Quote: ... a polymerase chain reaction using PfuUltra II Fusion DNA polymerase (Agilent Technologies), the template pIL-SVnisBTC and the appropriate pair of oligonucleotides (Supplementary File 4 ...
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bioRxiv - Immunology 2021Quote: ... The Long-Chain Fatty Acid Substrate Oxidation kit (Agilent cat #103672-100) was utilized to probe differences in OCR upon injection with either vehicle (media only ...
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bioRxiv - Molecular Biology 2023Quote: Site-directed mutagenesis used the QuikChange polymerase chain reaction (PCR) method (Stratagene). “Touchdown” PCR was typically performed over 18−20 cycles using the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Light chain mutants were generated using the QuikChange XL Mutagenesis kit (Stratagene) following the manufacturer’s instructions ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... TCR α and β chains were expressed in BL21 (DE3)-RIPL Escherichia coli cells (Agilent Technologies) following induction with 0.15 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: We used the Seahorse XF Long Chain Fatty Acid Oxidation Stress Test kit (Agilent, 102720-100). Cells were incubated in assay media supplemented with 150 µM BSA or oleic acid conjugated to BSA as fatty acid substrate in the following formulation ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Immunology 2022Quote: ... Vectors encoding the Fab light chains FenAB609 and FenAB709 were generated by QuikChange site-directed mutagenesis (Agilent QuikChange Lightning site-directed mutagenesis kit ...
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bioRxiv - Biochemistry 2021Quote: ... All mutant genes were constructed by polymerase chain reactions using a QuickChange Site-Directed Mutagenesis kit (Stratagene) and primers (Table S1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections of human tumors were stained with mouse anti-human CD68 (Dako) and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Plant Biology 2020Quote: ... Two DNA fragments were generated by polymerase chain reaction (PCR) using Herculase II Fusion DNA polymerase (Agilent Technologies), primers P11 + P12 and P13 + P14 and plasmids pUCAP-pGEL3::spmCherry-pATrpC-BAR (Samalova et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Immunology 2021Quote: Short chain fatty acid concentrations were determined using gas chromatography (3800 Varian GC, Agilent Technologies, Santa Clara, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... While the first polymerase chain reaction (PCR) was performed on a SureCycler 8800 (Agilent Technologies, Santa Clara, USA) using the following temperature profile ...
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bioRxiv - Systems Biology 2019Quote: ... and human TTR (Dako) with standard curves of purified human RBP4 (72 ...
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bioRxiv - Cancer Biology 2021Quote: ... Human universal RNA (Agilent) was used as a reference to standardise results between QPCR batches.
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bioRxiv - Biochemistry 2020Quote: The DNA template was amplified by the polymerase chain reaction (PCR) using Pfu high-fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of gDNA (∼27 ng ...
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bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... nucleotides: 907-1550) was amplified by the use of polymerase chain reaction (PCR) and cloned into pBluescript KS+ (Stratagene) via ClaI-EcoRI sites ...
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bioRxiv - Microbiology 2023Quote: ... Real-time Polymerase Chain Reaction (qPCR) was conducted in a Stragene Mx3005P real-time PCR system (Agilent Technologies®) to analyze the relative expression of the cytokine genes Interleukin 2 (IL-2) ...
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bioRxiv - Physiology 2023Quote: ... Hepatic portal vein bile acids and short chain fatty acids were acquired using an Agilent 1290 series HPLC (Agilent,) with an Acquity C18 BEH (2.1mm x 50mm ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human CD3 (Dako) and mouse anti-HIV-1 P24 (Dako) ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD44 (DAKO), Purified NA/LE mouse anti-human CD253 (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: Universal Human Reference RNA (Stratagene) was treated with RNase-Free DNase Set (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD8 (DAKO), and mouse anti-human TAG72 (AB16838 ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD3 (DAKO), mouse anti-human CD4 (DAKO) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD4 (DAKO), mouse anti-human CD8 (DAKO) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human universal total RNA (Agilent) was used as a positive control ...
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bioRxiv - Immunology 2023Quote: ... For human CD45 (Dako, M0701), CD19 (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...