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Citations for Agilent :
351 - 400 of 466 citations for EDG6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Extracted peptides were cleaned-up using automated C18 solid phase extraction on the Bravo AssayMAP platform (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: Immunohistochemical staining of formalin-fixed, paraffin-embedded xenografts was performed after antigen retrieval (120°C, 7 min at pH9) and peroxidase blocking (Dako) using the UltraVision LP detection system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were de-coverslipped with 1 min of agitation in TBST and subjected to heat-mediated antigen retrieval in 1x pH 6.0 citrate buffer (Biogenex Laboratories, HK0809K) for 20 min at 95°C followed by blocking of endogenous peroxidase activity (Dako, S2003 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2h at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: The cells were fixed with 4% paraformaldehyde at 4°C for 5 min and permeabilized with 0.1% Triton X-100 at room temperature for 20 min in the presence of a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hours at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... the blots were further incubated with a primary anti-total Tau antibody (rabbit Dako-A0024, 1:5000 in blocking buffer), then with the anti-rabbit HRP-conjugated secondary antibody ...
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bioRxiv - Physiology 2022Quote: ... Membranes were washed with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: swine anti-rabbit Ig HRP-conjugated (1:3000, catalog no. P0399, Dako) or goat anti-mouse Ig HRP-conjugated (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Cancer Biology 2021Quote: Biopsy sections were deparaffinized and antigens were retrieved by boiling in 10mM sodium citrate buffer pH6 for 40 minutes followed by endogenous biotin blocking (Agilent) and normal goat serum blocking (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with Dako biotin (cat. no. X0590) and peroxidase (cat. no. S200389) blocking reagents (both Agilent, Santa Clara, CA, USA), and incubated with primary antibodies (diluted in TBS-T-BSA ...
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bioRxiv - Neuroscience 2020Quote: ... the DAKO Autostainer Plus was used for a blocking step during 15 min at room temperature with a commercial wash buffer from DAKO supplemented with 3% normal goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... the neurons were rinsed three times with blocking buffer and then mounted onto slides using fluorescence mount medium (Agilent, USA). Fluorescence images were acquired as Z-sections at 1 μm × 10 sections with 488 nm (for NTR1 ...
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bioRxiv - Physiology 2022Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5% of goat normal serum (16210064 ...
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bioRxiv - Physiology 2022Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5% of goat normal serum (16210064 ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue was permeabolized with 0,5% Triton X-100 in PBS (PBS-T) and non-specific binding was blocked by incubating the tissue section with blocking reagent (DAKO). Antibody staining was performed in PBS-T with 1% blocking reagent (DAKO ...
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bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Pathology 2023Quote: ... the blocking solution was shaken off and the primary antibodies Arginase (Arg1 biocare cat#ACI3058) and CD68 (Dako cat#M0814) were applied ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hr at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were ionized by electrospray ionization and the ions were introduced into a 6510 Q-TOF mass spectrometer (Agilent). Spectra were analyzed with MassHunter Workstation Qualitative Analysis software version B.06.00 (Agilent).
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bioRxiv - Biochemistry 2021Quote: ... The peptide separation was performed on a C18 reverse phase column (Agilent, Poroshell 120, 0.3×35 mm, 2.7 μL) with a linear gradient of 8-48% B over 30 min (A ...
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bioRxiv - Microbiology 2019Quote: ... 1% acetonitrile/0.5% formic acid was used as eluent for 5 minutes to trap and desalt the peptides on the enrichment column (Zorbax SB C18, 0.3 × 5 mm, Agilent). An acetonitrile/0.1% formic acid gradient from 5% to 40% acetonitrile was then used within 120 minutes to separate the peptides on a Zorbax 300 SB C18 ...
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bioRxiv - Immunology 2022Quote: ... and the resulting peptides were captured and desalted by C-8 trap column (2.1 × 20 mm, Zorbax Eclipse XDB-C8 trap (Agilent) followed by loading on to C-18 column (2.1 × 50 mm in size ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide solutions were desalted using OMIX C18-micro solid phase extraction (SPE) columns (Agilent, Santa Clara, CA, USA). A Q-Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... alkylated eluents were digested with trypsin at 37°C and the extracted peptides were then cleaned with ZipTips (Agilent) and dried in a SpeedVac (VWR) ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μg of peptides were loaded unto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Synthetic Biology 2020Quote: Mass spectrometry analysis of tryptic peptides was conducted on a 6460 Triple Quad LC-MS system (Agilent Technologies, USA). From the samples prepared according to the protocol described above ...
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bioRxiv - Microbiology 2020Quote: ... Solid phase extraction of peptides was performed using C18 reversed phase sorbent containing 100 µL pipette tips according to the manufacturer’s instructions (Agilent, Santa Clara ...
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bioRxiv - Biophysics 2019Quote: ... samples were passed through an immobilized pepsin column (2mm × 2cm) at 50μl min-1 and the digested peptides were captured on a 2mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Biochemistry 2020Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 50 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were then desalted with Oasis HLB plates (Waters) and fractionated with an Agilent 1200 HPLC pump system (Agilent) coupled to a strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Digested peptides were separated on ZORBAX 300SB-C18 MicroBore column (1.0 x 50 mm, 3.5mm, Agilent Technologies, DE, USA) with a linear gradient of acetonitrile from 0 to 50% at 1% per min with a flow rate of 20 ml/min ...
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bioRxiv - Cell Biology 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 mL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were further separated using a Zorbax 300SB-C18 column (0.075×150 mm, 3.5 μm 300 Å) (Agilent Technologies) at a flow rate of 0.25 μL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was done in Primary Blocking Buffer (10% donkey serum, 0.1% saponin, 0.05 mg/mL sonicated salmon sperm DNA (Stratagene, La Jolla, California) in PBS for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... lyophilized samples were resuspended in Buffer A (5 mM NH4HCO2/ 2% ACN) and 5 mg peptide material (5 mg/ml) was loaded onto a reversed phase column (ZORBAX 300Extend-C18, Agilent). Peptides were separate at a flow rate of 2 ml/min and a constant column temperature of 40 °C using a binary buffer system ...
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bioRxiv - Cell Biology 2021Quote: Ap-proximately 1000 ng of peptides from each biological replicate before SCX fractionation were first injected onto an Agilent 1290 Infinity UHPLC system (Agilent) on a 50-cm analytical column packed with C18 beads (Dr Maisch Reprosil C18 ...
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bioRxiv - Genomics 2022Quote: ... The total peptide concentration was measured by UV absorbance at 210 nm using an LC system with UV detector (Agilent), with roughly 10 mg tissue yielding 500-750 μg peptide mixture.
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bioRxiv - Bioengineering 2021Quote: Peptides used for pMHC refolding were synthesized in housed using the Liberty Blue Peptide Synthesizer (CEM) and validated using LC-MS (Agilent). To generate pMHC molecules for bioconjugation ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 200 µg of dried peptides were subjected to phosphopeptide enrichment using IMAC cartridges and a BRAVO AssayMAP liquid handling platform (Agilent) as recently described 47.
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...